Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. In some aspects, the disclosure provides compositions comprising luminescently labeled nucleotides.

Abstract: Aspects of the application provide compounds of formula (I): or a salt thereof, which may be useful as chromophores and/or fluorophores for labeling highly water-soluble biomolecules (e.g., proteins, polypeptides, nucleotides, or oligonucleotides).

Abstract: Aspects of this disclosure relate to luminescently labeled nucleic acid nanostructures and methods and compositions for the detection and/or sequencing of one or more target molecules derived from a sample.

Abstract: Techniques for multi-dimensional signal analysis are described herein. The techniques may be used in one or more sequencing applications. For example, according to some aspects, there is provided a method comprising: determining information about a sample that emits emission light in response to excitation light based on at least one of pulse duration and interpulse duration and at least two of wavelength, intensity, and lifetime of the emission light, wherein the sample comprises a reagent configured to be coupled to a luminescent label, and wherein a shielding element is disposed between the reagent and the luminescent label.

Abstract: The present disclosure relates to compounds and methods for selective C-terminal functionalization of peptides. In certain embodiments, the compounds have improved water-solubility, and are suitable for use in connection with peptide sequencing methodologies.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: The present disclosure is related to peptides comprising modified aspartic acid and glutamic acid moieties, methods of making such peptides, and methods of using such modified peptides to selectively direct cleavage of peptide bonds. Selective peptide bond cleavage is advantageous in peptide sequencing applications, such as automated peptide sequencing applications.

Abstract: A method and device for image processing, a method for training an object detection model are provided in the present disclosure. In the method for image processing, a visible light image is acquired. A central weight map corresponding to the visible light image is generated. Weight values represented by the central weight map gradually decrease from a center to an edge of a visible light image. The visible light image and the central weight map are inputted into an object detection model to obtain an object region confidence map. The object detection model is a model obtained by training according to multiple set of training data, each set of which includes a visible light image, a central weight map and a corresponding labeled object mask pattern for a same scenario. A target object in the visible light image is determined according to the object region confidence map.

Abstract: The present disclosure provides a virtual object grouping method and device, a storage medium, and an electronic device. The method includes: in response to a grouping instruction, an operation state for grouping the at least one virtual object in a predetermined display area is activated; in response to a first selection instruction, at least one target virtual object to be grouped is selected from the at least one virtual object; in response to a second selection instruction, a target group is selected from at least one pre-created group; and the at least one target virtual object to be grouped is grouped into the target group. By means of embodiments of the present disclosure, the problems of small player operation space and low game operability in the related art are solved.

Abstract: Methods and devices for ultrasensitive detection of target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices enable ultrasensitive determination of the concentration of target molecules.

Abstract: Aspects of the disclosure relate to methods and systems for regenerating a sensor chip surface, including techniques for reuse of a single sensor chip in multiple sampling cycles by regenerating a surface of the sensor chip between successive sampling cycles. A method is provided for reusing an integrated device to process a sample, the sample being divided into a plurality of aliquots, the method comprising: loading a first aliquot of the plurality of aliquots into at least some of a plurality of chambers of the integrated device; sampling analytes of the first aliquot while the analytes are present in the at least some of the plurality of chambers; removing the first aliquot from the at least some of the plurality of chambers of the integrated device; and loading a second aliquot of the plurality of aliquots into the at least some of the plurality of chambers of the integrated device.

Abstract: The present disclosure relates to compounds and methods for selective C-terminal functionalization of peptides. In certain embodiments, the compounds have improved water-solubility, and are suitable for use in connection with peptide sequencing methodologies.

Abstract: Aspects of the disclosure relate to techniques for determining a measure of quantitative loading of a sample in an integrated device. According to some embodiments, there is provided a method for determining a measure of quantitative loading of a sample in an integrated device, the method comprising exciting, with excitation light from at least one excitation source, one or more reference dye molecules that, during the exciting with the excitation light, are attached to respective biomolecules of the sample bound to a surface of a chamber of one or more chambers of the integrated device, obtaining a signal emitted by the one or more reference dye molecules in response to the excitation light, and determining, based on the signal emitted by the one or more reference dye molecules, the measure of quantitative loading of the sample.

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

Abstract: The present disclosure relates to the field of genetic engineering, in particular to a recombinant human 2Ig-B7-H3 protein encoding gene, a protein, a recombinant vector, and a pharmaceutical composition including the gene or protein. The recombinant human 2Ig-B7-H3 protein encoding gene comprises a nucleotide sequence shown in SEQ ID NO:1.

Abstract: The present disclosure relates to the field of bio-pharmacy, in particular, to an expression and/or overexpression vector, a pharmaceutical composition and a method for preparing the same. The expression and/or overexpression vector of the present disclosure includes a human glucokinase mutant-encoding gene and an adeno-associated virus vector. The pharmaceutical composition of the present disclosure includes virus particles obtained by packaging the expression and/or overexpression vector. After the pharmaceutical composition of the present disclosure is injected into an animal, an obvious expression and/or overexpression of a glucokinase mutant may occur, which has a good blood glucose level lowering effect, and provides a powerful new approach for controlling/stabilizing blood glucose level and/or preventing and treating glucose metabolism disorders, especially for preventing and treating diabetes.