Patents by Inventor Hakho Lee

Hakho Lee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210080390
    Abstract: The disclosure features systems and methods for measuring and diagnosing target constituents bound to labeling particles in a sample. The systems include a radiation source, a sample holder, a detector configured to obtain one or more diffraction patterns of the sample each including information corresponding to optical properties of sample constituents, and an electronic processor configured to, for each of the one or more diffraction patterns: (a) analyze the diffraction pattern to obtain amplitude information and phase information corresponding to the sample constituents; (b) identify one or more particle-bound target sample constituents based on at least one of the amplitude information and the phase information; and (c) determine an amount of at least one of the particle-bound target sample constituents in the sample based on at least one of the amplitude information and the phase information.
    Type: Application
    Filed: July 27, 2020
    Publication date: March 18, 2021
    Inventors: Ralph Weissleder, Hakho Lee, Hyungsoon Im, Cesar Castro
  • Publication number: 20200408687
    Abstract: In an exemplary method, a sample is provided. The sample contains a polymerase molecule, a receptor molecule containing a fluorophore, and a detector molecule. The detector molecule is configured to, in the presence of a target nucleic acid, inhibit the polymerase molecule from cleaving the fluorophore. A level of fluorescence anisotropy from the sample is detected to determine the absence or presence of the target nucleic acid in the sample.
    Type: Application
    Filed: March 27, 2017
    Publication date: December 31, 2020
    Inventors: Ralph Weissleder, Hakho Lee, Kisoo Park, Chen-han Huang
  • Patent number: 10724948
    Abstract: The disclosure features systems and methods for measuring and diagnosing target constituents bound to labeling particles in a sample. The systems include a radiation source, a sample holder, a detector configured to obtain one or more diffraction patterns of the sample each including information corresponding to optical properties of sample constituents, and an electronic processor configured to, for each of the one or more diffraction patterns: (a) analyze the diffraction pattern to obtain amplitude information and phase information corresponding to the sample constituents; (b) identify one or more particle-bound target sample constituents based on at least one of the amplitude information and the phase information; and (c) determine an amount of at least one of the particle-bound target sample constituents in the sample based on at least one of the amplitude information and the phase information.
    Type: Grant
    Filed: July 2, 2018
    Date of Patent: July 28, 2020
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Hyungsoon Im, Cesar Castro
  • Patent number: 10712343
    Abstract: This invention relates to methods for diagnosing cancer, e.g., cancer of epithelial origin, by detecting the presence of tumor cells in a sample, based (at least in some embodiments) on the quantification of levels of four biomarkers, MUC1, EGFR, EpCAM, and HER2. In some embodiments, the methods are performed using diagnostic magnetic resonance (DMR), e.g., with a portable relaxometer or MR imager.
    Type: Grant
    Filed: January 9, 2017
    Date of Patent: July 14, 2020
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Cesar Castro
  • Patent number: 10557847
    Abstract: Disclosed herein are compositions and methods for exosome detection with high sensitivity by using a nano-plasmonic sensor. The nano-plasmonic sensor comprises a plurality of nanoapertures suitable for transmission measurements. The detection sensitivity is on the order of 104-fold higher than western blotting and 102-fold higher than enzyme-linked immunosorbent assay (ELISA). A portable imaging system is also disclosed, enabling rapid and high-throughput detection of exosomes. The nano-plasmonic sensor and imaging system can be useful in diagnostics.
    Type: Grant
    Filed: December 2, 2014
    Date of Patent: February 11, 2020
    Assignee: THE GENERAL HOSPITAL CORPORATION
    Inventors: Ralph Weissleder, Hakho Lee, Hyungsoon Im, Cesar Castro
  • Publication number: 20190346434
    Abstract: Target analyte detection devices include a housing having a potentiostat and a microcontroller coupled to the potentiostat. The device also includes a substrate having a plurality of electrodes on a first surface of the substrate. A first set of electrodes of the plurality of electrodes defines a first sample detection region. The substrate is removably attachable to the housing such that the first set of electrodes is coupled to the potentiostat upon attaching the substrate to the housing. The device also includes a magnet assembly couplable to a second surface of the substrate. The magnet assembly includes a magnet positioned in the magnet assembly such that a magnetic field from the magnet extends through the substrate and the first set of electrodes into an area above the first sample detection region upon coupling the magnet assembly to the substrate.
    Type: Application
    Filed: January 27, 2017
    Publication date: November 14, 2019
    Inventors: Hakho Lee, Ralph Weissleder, Sangmoo Jeong, Jongmin Park, Caesar Castro, Hsing-Ying Lin, Jamil Azzi
  • Publication number: 20190137494
    Abstract: In one aspect, a system for isolating particles includes a first array of magnets, a second array of magnets arranged generally in parallel with and spaced apart from the first array of magnets, and a microfluidic device. The microfluidic device includes a substrate, an inlet arranged on the substrate and configured to receive a fluid sample, an outlet arranged on the substrate, a first region of the substrate including a channel connected to the inlet, where the first region of the substrate is arranged to sandwich the channel between the first and second arrays of magnets, and a second region of the substrate in fluid communication with the channel and including a particle capture zone containing a plurality of particle capture sites, where each particle capture site including a receptacle sized to confine a first type of particle and an opening in fluid communication with the receptacle, wherein a size of the receptacle is larger than a size of the opening.
    Type: Application
    Filed: December 10, 2018
    Publication date: May 9, 2019
    Inventors: Hakho Lee, Ralph Weissleder, Kyungheon Lee, Jun Song
  • Patent number: 10274490
    Abstract: The present invention provides novel methods of magnetically labeling a bacterial cell by contacting the call with an affinity ligand and subsequently contacting the cell with a magnetic agent, where the affinity ligand and magnetic agent include bioorthogonally reactive groups that can react with each other to form a covalent bond. Compounds, compositions, kits and applications of the method are also described.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: April 30, 2019
    Assignee: The General Hospital Corporation
    Inventors: Ghyslain Budin, Ralph Weissleder, Hakho Lee, Hyun Jung Chung
  • Publication number: 20190113451
    Abstract: The disclosure features systems and methods for measuring and diagnosing target constituents bound to labeling particles in a sample. The systems include a radiation source, a sample holder, a detector configured to obtain one or more diffraction patterns of the sample each including information corresponding to optical properties of sample constituents, and an electronic processor configured to, for each of the one or more diffraction patterns: (a) analyze the diffraction pattern to obtain amplitude information and phase information corresponding to the sample constituents; (b) identify one or more particle-bound target sample constituents based on at least one of the amplitude information and the phase information; and (c) determine an amount of at least one of the particle-bound target sample constituents in the sample based on at least one of the amplitude information and the phase information.
    Type: Application
    Filed: July 2, 2018
    Publication date: April 18, 2019
    Inventors: Ralph Weissleder, Hakho Lee, Hyungsoon Im, Cesar Castro
  • Patent number: 10151753
    Abstract: In one aspect, a system for isolating particles includes a first array of magnets, a second array of magnets arranged generally in parallel with and spaced apart from the first array of magnets, and a micro fluidic device. The micro fluidic device includes a substrate, an inlet arranged on the substrate and configured to receive a fluid sample, an outlet arranged on the substrate, a first region of the substrate including a channel connected to the inlet, where the first region of the substrate is arranged to sandwich the channel between the first and second arrays of magnets.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: December 11, 2018
    Assignee: The General Hospital Corporation
    Inventors: Hakho Lee, Ralph Weissleder, Kyungheon Lee, Jun Song
  • Patent number: 10111971
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: October 30, 2018
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Patent number: 10012589
    Abstract: The disclosure features systems and methods for measuring and diagnosing target constituents bound to labeling particles in a sample. The systems include a radiation source, a sample holder, a detector configured to obtain one or more diffraction patterns of the sample each including information corresponding to optical properties of sample constituents, and an electronic processor configured to, for each of the one or more diffraction patterns: (a) analyze the diffraction pattern to obtain amplitude information and phase information corresponding to the sample constituents; (b) identify one or more particle-bound target sample constituents based on at least one of the amplitude information and the phase information; and (c) determine an amount of at least one of the particle-bound target sample constituents in the sample based on at least one of the amplitude information and the phase information.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: July 3, 2018
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Hyungsoon Im, Cesar Castro
  • Patent number: 9958416
    Abstract: Determining a presence of a target analyte in a fluid sample includes mixing multiple magnetic particles with the fluid sample, in which the magnetic particles are each bound to one or more binding moieties that specifically bind to the target analyte, flowing the fluid sample containing the magnetic particles through a fluidic channel, exposing the fluid sample in the fluidic channel to a magnetic field, measuring a signal from a Hall effect sensor while the fluid sample flows through the fluidic channel, and determining whether the target analyte is present in the fluid sample when the measured signal is in a first range of values.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: May 1, 2018
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Patent number: 9874564
    Abstract: A portable magnetic resonance system includes a permanent magnet, a nuclear magnetic resonance probe, and control electronics. The control electronics are configured to transmit to the probe a magnetic resonance excitation signal having an excitation frequency f, receive from the probe a magnetic resonance measurement signal, detect in the magnetic resonance measurement signal a magnetic resonance frequency f0, and automatically adjust the excitation frequency f until the difference between the excitation frequency and the magnetic resonance frequency is approximately equal to a target offset.
    Type: Grant
    Filed: March 21, 2012
    Date of Patent: January 23, 2018
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Patent number: 9869619
    Abstract: A magnetic device includes a substrate and an array including a plurality of magnetic particles, in which a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles in the array alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle or directly adjacent magnetic particles in the array.
    Type: Grant
    Filed: December 29, 2016
    Date of Patent: January 16, 2018
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Publication number: 20170216463
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Application
    Filed: April 14, 2017
    Publication date: August 3, 2017
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Publication number: 20170212021
    Abstract: A magnetic device includes a substrate and an array including a plurality of magnetic particles, in which a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles in the array alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle or directly adjacent magnetic particles in the array.
    Type: Application
    Filed: December 29, 2016
    Publication date: July 27, 2017
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Publication number: 20170122951
    Abstract: This invention relates to methods for diagnosing cancer, e.g., cancer of epithelial origin, by detecting the presence of tumor cells in a sample, based (at least in some embodiments) on the quantification of levels of four biomarkers, MUC1, EGFR, EpCAM, and HER2. In some embodiments, the methods are performed using diagnostic magnetic resonance (DMR), e.g., with a portable relaxometer or MR imager.
    Type: Application
    Filed: January 9, 2017
    Publication date: May 4, 2017
    Inventors: Ralph Weissleder, Hakho Lee, Cesar Castro
  • Patent number: 9623126
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: April 18, 2017
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Patent number: 9532729
    Abstract: A magnetic device includes a substrate and an array including a plurality of magnetic particles, in which a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles in the array alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle or directly adjacent magnetic particles in the array.
    Type: Grant
    Filed: September 27, 2011
    Date of Patent: January 3, 2017
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore