Abstract: A method and system for removing from an aqueous system which is contaminated therewith: (1) mercury present as colloids, ions and/or organically bound compounds, and (2) hydrocarbons solubilized, dispersed, and/or emulsified in the said system. Pursuant to the invention the aqueous system to be treated (such as “produced water”) is passed successively through three filtration stages. The first filtration stage is provided with absorption media which effects reduction/removal of dispersed organically bound mercury species and of the dispersed and partially dissolved hydrocarbon phases, as well as of some colloidal mercury and other dissolved metallic species. The second filtration stage utilizes a salt modified reticulated granular filtration media for reduction/removal of slightly dissolved hydrocarbon phases, mercury in colloidal and ionic form and other dissolved metals.

Abstract: A method and apparatus for removing organic contaminants from an aqueous phase in which the contaminant is solubilized. In the method the aqueous phase is passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The absorbtion composition is cured in situ at the filter. The contaminant is immobilized at the media, and the purified filtrate having passed through the filtration media is collected as the product. The said oil component comprises at least 95% by weight of the combined oil and polymer components.

Abstract: A method and apparatus for removing organic contaminants from an aqueous phase in which the contaminant is solubilized. In the method the aqueous phase is passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The absorbtion composition is cured in situ at the filter. The contaminant is immobilized at the media, and the purified filtrate having passed through the filtration media is collected as the product. The media comprises a central core which is surrounded and wrapped by a plurality of overlying sheets of further fluid pervious media, the overlying sheets creating void spaces between sheets for trapping of at least some of the separated contaminants.

Abstract: The present invention relates to expression vectors, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of the wild-type promoter and cells comprising the same. Methods utilizing either the vectors or cells of this invention, in optimizing regulation of gene expression, protein expression, or optimized gene or protein delivery are described.

Abstract: The invention relates to global transcription machinery engineering to produce altered cells having improved phenotypes.

Abstract: The present invention relates to expression cassettes libraries of expression vectors comprising the same, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of another in the library and cells comprising the same. Methods utilizing either the libraries or cells of this invention, in optimizing gene expression, protein expression, or optimized gene or protein delivery are described.

Abstract: The present invention provides genetically manipulated cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD, hnr or yjfP gene, or further disrupted for a gdhA, gpmB aceE, ppc, talB or fdhF gene, or any combination thereof, or cells inhibited for their expression, activity or function are disclosed. Methods of enhanced carotenoid synthesis utilizing cells genetically engineered to overexpress dxs, idi, yjiD, rpoS, torC, appY, ydgK, yeiA, yedR, tort, arcB, yggT, purDH, yfjN or a combination thereof, or further disrupted for the above-referenced genes are disclosed. Methods for identifying genes involved in optimized production of a carotenoid, and cells disrupted for, or inhibited for the expression, activity or function of genes thus identified are described.

Abstract: A multistage filtration system filtration apparatus for separating organic contaminants from an aqueous phase in which the contaminant is solubilized or emulsified. Two or more filtration stages are connected in series, each stage comprising a canister or other container having an inlet and an outlet for passing said liquid phase therethrough, the aqueous phase thereby passing through the series connected stages. A fluid-pervious media is provided at the interior of the canister or container in the flow path of the liquid phase proceeding between said inlet and outlet, the media being infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, with a methacrylate or acrylate polymer component. The absorption composition is cured in situ at the media.

Abstract: The present invention provides engineered cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD, hnr or yjfP gene, or further disrupted for a gdhA, gpmB, aceE, ppc, talB or fdhF gene, or any combination thereof, or cells inhibited for their expression, activity or function are disclosed. Additionally, methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for gdhA, aceE, fdhF, yjiD, hnr or yjfP gene expression or any combination thereof and ackA, appY, aspC, clp, clpP, clpXP, crcB, csdA, cyaA, evgS, fdhA, fdhD, feoB, funA, glnE, glxR, gntK, hycI, lipB, lysU, modA, moeA, nadA, nuoC, nuoK, pflB, pitA, pst, pstC, pta, p-yjiD, sohA, sipA, yagR, yaiD, ybaS, ycfZ, ydeN, yebB, yedN, yfcC, ygjP, yibD, yjfP, yjhH, or yliE gene expression, or a combination thereof or cells inhibited for their expression, activity or function are disclosed.

Abstract: A method and apparatus for removing organic contaminants from an aqueous phase in which the contaminant is solubilized. In the method the aqueous phase is passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising (1) a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component; and (2) a photoinitiator system; said absorbtion composition being cured in situ at the filter by exposure to actinic radiation. The contaminant is immobilized at said media, and the purified filtrate having passed through the filtration media is collected as the product.

Abstract: A method and apparatus for removing organic contaminants from an aqueous phase in which the contaminant is solubilized. In the method the aqueous phase is passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The absorbtion composition is cured in situ at the filter. The contaminant is immobilized at the media, and the purified filtrate having passed through the filtration media is collected as the product. The media comprises a central core which is surrounded and wrapped by a plurality of overlying sheets of further fluid pervious media, the overlying sheets creating void spaces between sheets for trapping of at least some of the separated contaminants.

Abstract: A method and apparatus for removing organic contaminants from an aqueous phase in which the contaminant is solubilized. In the method the aqueous phase is passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The absorbtion composition is cured in situ at the filter. The contaminant is immobilized at the media, and the purified filtrate having passed through the filtration media is collected as the product. The said oil component comprises at least 95% by weight of the combined oil and polymer components.

Abstract: A method for separating undesired particles from a gaseous medium in which the particles are dispersed. The particles and gaseous medium are passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The particles are thereby immobilized at the media. The purified gaseous medium having passed through the filtration media is collected and flowed to a utilization or discharge point.

Abstract: A method for separating a more hydrophobic grease or oil contaminant from a less hydrophobic aqueous coolant/lubricant emulsion in which the contaminant has accumulated and in which it is itself emulsified. The contaminated coolant/lubricant is passed through a fluid pervious filtration media which has been infused with an absorption composition which is a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The contaminant is immobilized at the media and preferentially retained at the filter; and the purified filtrate having passed through the filtration media is collected for further use.

Abstract: A method for separating undesired particles from a gaseous medium in which the particles are dispersed. The particles and gaseous medium are passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The particles are thereby immobilized at the media. The purified gaseous medium having passed through the filtration media is collected and flowed to a utilization or discharge point.

Abstract: A method for improving the efficiency and usable life in a cross flow membrane filter used to remove contaminants from bilgewater. The bilgewater is passed upstream of the cross filter membrane through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The contaminants are thereby immobilized at the media. The purified output from the infused filtration media is then provided as input for the cross flow membrane filter.

Abstract: A method for improving the efficiency and usable life in a cross flow membrane filter used to remove contaminants from bilgewater. The bilgewater is passed upstream of the cross filter membrane through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The contaminants are thereby immobilized at the media. The purified output from the infused filtration media is then provided as input for the cross flow membrane filter.

Abstract: Filtration media for removing organic contaminants from aqueous systems contacted therewith. The media comprises granulated activated carbon which has been infused with an absorption composition which is a homogenous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component.

Abstract: A method and apparatus for removing organic contaminants from an aqueous phase in which the contaminant is solubilized. In the method the aqueous phase is passed through a fluid-pervious filtration media which has been infused with an absorbtion composition comprising a homogeneous thermal reaction product of an oil component selected from the group consisting of glycerides, fatty acids, alkenes, and alkynes, and a methacrylate or acrylate polymer component. The contaminant is thereby immobilized at said media, and the purified filtrate having passed through the filtration media is collected as the product.

Abstract: The invention discloses methods of making oil coagulant compositions, the oil coagulants made thereby and processes for using the coagulants to coagulate oil that has been spilled on water. The coagulant is made from a glyceride, such as, for example, the drying oil linseed oil, a polymer, such as, for example, isobutyl methacrylate polymer, and a solvent, such as, for example, 2,2,4-trimethyl-1,3-pentanediol monoisobutyrate. The composition of the present invention floats on the water surface and coagulates oil independent of both agitation and temperature, and can be used in both salt and fresh water. After the coagulant has coagulated the spilled oil, the floating coagulated oil can be readily mechanically removed from the water such that at least 99.9% of the oil is removed from the water and only a faint trace of oil remains in the water. An alternate way of using the composition is to impregnate it into a porous substrate, which substrate is then applied to the spilled oil.