Abstract: Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co-aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co-precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods.

Abstract: The present invention relates to a real-time PCR monitoring apparatus for real-time monitoring production of reaction product produced during the reaction while performing nucleic acid amplification such as PCR for various kinds of trace samples. Specifically, the present invention relates to an apparatus for real-time monitoring biochemical reaction for efficiently dividing interference between an excitation light and a fluorescence, which includes a polarizer, a polarizing beam splitter, a polarization converter and so on.

Abstract: A method of preparing siRNAs for selective inhibition of target mRNA isotypes comprises: dividing target mRNA isotypes intended to inhibit the expression thereof and non-target mRNA isotypes from the mRNA isotypes of a gene; allotting a common location information region (A) of exons on genome DNA corresponding to the target mRNA isotypes; allotting a location information region (B) present specifically in exons of genome DNA corresponding to target mRNAs by excluding the location information region of exons on genome DNA corresponding to non-target mRNA from the location information region (A); determining base sequences in the target mRNAs corresponding to the location information region (B); and obtaining siRNA sequences for inhibiting the determined base sequences specifically.

Abstract: The present invention relates to equipment for measuring water quality using a fish or a water flea, an more particularly, to equipment for measuring water quality in which small organisms such as killifish (or medaka) or water flea are put into test chamber having a plurality of divided sections and behavior of the fishes or the water fleas accommodated in the test chamber is observed while continuously flowing water of a river or a water zone to be examined through the test chamber, thereby finding out and measuring existence of toxic element or harmful substance included in the water to be examined.

Abstract: A inhibition method of target mRNA expression includes: (a) obtaining binding energy of a double combination section on a dsRNA sequence of all combination comprising complementary nucleotides to a random target mRNA; (b) dividing the binding energy into four sections on the dsRNA sequence of each combination to obtain a difference of the mean binding energy between each section and convert into a score of a relative combination energy pattern; (c) selecting siRNA whose inhibition efficiency to target mRNA is expected to be high by applying the converted score to the dsRNA sequence with other factors that affect the efficiency of siRNA; and (d) inhibiting target mRNA expression using the selected siRNA.

Abstract: The present invention relates to a dried oligonucleotide composition and a method for producing the same. More specifically, it relates to a dried oligonucleotide composition produced by the steps comprising adding a substance for preventing the oligonucleotide from being separated and lost, which is adhesive to a storage container containing the oligonucleotide composition, in order to prevent the oligonucleotide from being separated and lost during manufacturing and distributing the dried oligonucleotide composition, optionally adding a non-reactive dye substance, and drying the resulting solution. The dried oligonucleotide composition of the present invention can be prevented from being separated and lost during manufacturing step, or transporting step after packaging, and the presence or absence of the oligonucleotide in the storage container can be easily confirmed with naked eyes.

Abstract: The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four (4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931 C to 96 C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C.

Abstract: The present invention relates a process of quality examining for microarray of biological material. More particularly, the present invention is directed to a process of quality examining for microarray of biological material, which comprises 1) a step for mixing probe and a compound which emits light or heat and does not react with said probe, 2) a step for microarraying the mixture obtained in step 1) on a substrate, and 3) a step for measuring light or heat emitted by the scanning of each spots of the microarray mixture.

Abstract: The present invention relates to an apparatus for real-time monitoring chemical reaction between various biomaterials. More particularly, the present invention directed to a real-time monitoring apparatus for biochemical reaction, which comprises parabolic mirror and/or an optical waveguide tube for effective irradiation of light over the whole plate with uniform intensity.

Abstract: The present invention relates to a method for identifying vehicle and oligonucleotide marker used therefor. More particularly, the present invention is directed to a method for identifying vehicle by using oligonucleotide to which phase transfer agent is bound, and oligonucleotide marker used therefor.

Abstract: The present invention relates to microorganisms for preventing or treating obesity or diabetes mellitus which have excellent viability and produce polysaccharides efficiently in the intestines. More particularly, is directed to microorganisms for preventing or treating obesity or diabetes mellitus, which are capable of reducing the amount of monosaccharides or disaccharides that can be absorbed into the intestine by converting those mono or disaccharides into polymeric materials that cannot be absorbed in the intestines. The present invention is also directed to a pharmaceutical composition containing pharmaceutically effective amount of the microorganisms and a food composition containing the microorganisms as an active ingredient. Preferred microorganisms are Lactobacillus reuteri KCTC-10301BP and Lactobacillus fermentum KCTC-10458BP.

Abstract: The present invention relates to a process for amplifying DNA of an organism. More particularly, the present invention is directed to a process for amplifying DNA of an organism through Polymerase Chain Reaction(PCR) without any information regarding a primer needed for amplifying DNA of an organism.

Abstract: The present invention relates to microorganisms for the treatment or the prevention of obesity or diabetes mellitus, which reduce the amount of monosaccharide or disaccharide which may be absorbed into human body by converting monosaccharides such as glucose, fructose, galactose et al. and disaccharides into polymeric materials which cannot be absorbed by the intestine, and relates to a pharmaceutical composition containing the said microorganisms. Preferred microorganisms are Lactobacillus sp. BC-Y009 and Acetobacter sp. BC-Y058.

Abstract: A method for obtaining DNA from fish spermatogonia comprises (i) disrupting fish spermatogonium to obtain a milky-white colloid, (ii) adding an alkaline solution of pH 8 to pH 12, containing more than 1 mol of salts, such as monovalent salts, to the milky-white colloid, to separate DNA from protamines, and then (iii) adding ethanol solution to the resultant mixture, effecting a precipitation of DNA.

Abstract: The present invention relates to a bio-molecular microchip and manufacturing process thereof. More particularly, the present invention is directed to a bio-molecular microchip comprising a substrate; and an amine group-containing bio-molecule immobilized onto a glycidyl moiety-containing polyacrylamide gel via covalent bonds formed between the glycidyl moiety of the polyacryl amide gel and the amine group of the bio-molecule by epoxy ring-opening reaction.

Abstract: The present invention relates to microorganisms for preventing or treating obesity or diabetes mellitus which have excellent viability and produce polysaccharides efficiently in the intestines, more particularly, is directed to microorganisms for preventing or treating obesity or diabetes mellitus, which are capable of reducing the amount of monosaccharides or disaccharides that can be absorbed into the intestine by converting those mono or disaccharides into polymeric materials that cannot be absorbed in the intestines. The present invention is also directed to a pharmaceutical composition containing pharmaceutically effective amount of the microorganisms and a food composition containing the microorganisms as an active ingredient.

Abstract: The present invention relates to a process for the preparation of full-length complementary DNA (cDNA).

Abstract: The present invention relates to microorganisms for the treatment or the prevention of obesity or diabetes mellitus, which reduce the amount of monosaccharide or disaccharide which may be absorbed into human body by converting monosaccharides such as glucose, fructose, galactose et al. and disaccharides into polymeric materials which cannot be absorbed by the intestine, and relates to a pharmaceutical composition containing the said microorganisms.

Abstract: The present invention relates to microorganisms for the treatment or the prevention of obesity or diabetes mellitus, which reduce the amount of monosaccharide or disaccharide which may be absorbed into human body by converting monosaccharides such as glucose, fructose, galactose et al. and disaccharides into polymeric materials which cannot be absorbed by the intestine, and relates to a pharmaceutical composition containing the said microorganisms.

Abstract: A method and apparatus for sterilizing and disinfecting bedclothes. The method includes preparing a sterilizing room defined by a cover; placing bedclothes into the sterilizing room; and exposing the bedclothes to ultraviolet rays and ozone generated by a generator in communication with the sterilizing room. The apparatus includes a generator for generating ultraviolet rays and ozone and a cover for defining a sterilizing room to accommodate the bedclothes.