Abstract: The present invention relates to a process for the preparation of full-length complementary DNA (cDNA).

Abstract: The present invention relates to an automatic DNA isolation apparatus which comprises liquid containers; numerous liquid flow passages; control valves; numerous syringes; numerous injection needles; transportation means; vacuum block; plate rack (31); numerous multi-well plate blocks; and process control unit which regulates the operation of said control valves for selection and transfer of said various liquids, the operation of said syringe for quantitative intake and discharge of said liquids, the transfer of said injection needle connected with said syringes, the sequential transportation of said multi-well plates arranged in said multi-well plate rack, the sequential operation of shaking block and said vacuum block.

Abstract: The present invention relates to a process for the preparation of full-length complementary DNA (cDNA).

Abstract: The present invention relates a process of quality examining for microarray of biological material. More particularly, the present invention is directed to a process of quality examining for microarray of biological material, which comprises 1) a step for mixing probe and a compound which emits light or heat and does not react with said probe, 2) a step for microarraying the mixture obtained in step 1) on a substrate, and 3) a step for measuring light or heat emitted by the scanning of each spots of the microarray mixture.

Abstract: The present invention relates to a process for amplifying DNA of an organism. More particularly, the present invention is directed to a process for amplifying DNA of an organism through Polymerase Chain Reaction(PCR) without any information regarding a primer needed for amplifying DNA of an organism.

Abstract: The present invention relates to a bio-molecular microchip and manufacturing process thereof. More particularly, the present invention is directed to a bio-molecular microchip comprising a substrate; and an amine group-containing bio-molecule immobilized onto a glycidyl moiety-containing polyacrylamide gel via covalent bonds formed between the glycidyl moiety of the polyacryl amide gel and the amine group of the bio-molecule by epoxy ring-opening reaction.

Abstract: The present invention relates to an apparatus for automatic measuring of water toxicity. More specifically, the present invention provides an apparatus for automatic and continuous measuring of water toxicity and/or contamination using luminescent microorganisms living in freshwater.

Abstract: A method for obtaining DNA from fish spermatogonia comprises (i) disrupting fish spermatogonium to obtain a milky-white colloid, (ii) adding an alkaline solution of pH 8 to pH 12, containing more than 1 mol of salts, such as monovalent salts, to the milky-white colloid, to separate DNA from protamines, and then (iii) adding ethanol solution to the resultant mixture, effecting a precipitation of DNA.

Abstract: The present invention relates to microorganisms for the treatment or the prevention of obesity or diabetes mellitus, which reduce the amount of monosaccharide or disaccharide which may be absorbed into human body by converting monosaccharides such as glucose, fructose, galactose et al. and disaccharides into polymeric materials which cannot be absorbed by the intestine, and relates to a pharmaceutical composition containing the said microorganisms.

Abstract: The present invention relates to a multi-channel quantitative control valve apparatus which controls transfer of various kinds of solutions, selectively and quantitatively. More particularly, the present invention relates to a multi-channel quantitative control valve apparatus which comprises multichannel membrane valves which control selective transfer of solutions, numerous syringes which imbibe or discharge quantitatively the selected solution, and numerous liquid flow passages.

Abstract: The present invention relates to an apparatus for automatic measuring of water toxicity. More specifically, the present invention provides an apparatus for automatic and continuous measuring of water toxicity and/or contamination using luminescent microorganisms living in freshwater.

Abstract: The system opens and closes an n×m array of liquid pathways using n+m valves. The pathways (2) run from top to bottom of a body (1). Control bars (4), also with liquid pathways (2), are inserted in channels (3). These are movable by a power source. The liquid pathways are opened to pass liquid when the pathways in the body and the control bars are aligned with each other.

Abstract: The present invention provides a lyophilized reagent for PCR which is prepared by adding a stabilizing and sedimenting agent to an aqueous reaction mixture and lyophilizing thereof. The lyophilized PCR reagent of the present invention leads to a simplification of multi-step PCR manipulation, an increase of heat stability of the reaction mixture, prevention of carry-over contamination, and improved credibility of experiments. The lyophilized PCR reagent can be applied as a kit for analysis of DNA sequence or for diagnosis of diseases, which guarantee the results of high credibility in a short period of time.

Abstract: The present invention provides an improved method for silver staining of DNA which comprises the steps of linking DNA to a polymer which has a high affinity for the DNA and binding of a plenty of silver ions to the DNA-polymer complex. The silver staining method of the present invention can be applied for the detection and sequencing of trace amount of DNA.

Abstract: The present invention provides a process for preparing a ready-to-use reagent for PCR, which comprises the steps of adding the stabilizer, glucitol or glucose, to an aqueous polymerase chain reaction(PCR) mixture containing a reaction buffer, MgCl.sub.2, dNTPs, a DNA polymerase, the water soluble dye, bromophenol blue, xylene cyanole, bromocresol red or cresol red, a primer, and optionally, ddATP, ddCTP, ddGTP, or ddTTP, and then lyophilizing thereof. The ready-to-use PCR reagent of the present invention leads to a simplification of multi-step PCR manipulation, an increase of heat stability of the reaction mixture, prevention of carry-over contamination, and improved credibility of experiments. The ready-to-use PCR reagent can be applied as a kit for analysis of DNA sequence or for diagnosis of diseases.