Abstract: The present invention provides a transformant having a gene expression inducing system having better responsiveness. The transformant of the invention has a galactose induction system and retains a coding region having a galactose induction system and encoding an assisting protein assisting transcription activation by a protein having the Gal4 activity under control of a constitutive promoter, and a coding region encoding an endogenous or exogenous desired protein under control of a galactose-inducible promoter.

Abstract: To include a focused ion beam apparatus fabricating a sliced specimen by processing a specimen as well as observing the sliced specimen, a scanning electron microscope observing the slice specimen, a gas-ion beam irradiation apparatus performing finishing processing by irradiating a gas-ion beam onto a surface of the sliced specimen, a specimen stage on which the sliced specimen is fixed and having at least one or more rotation axis, a specimen posture recognition means recognizing positional relation of the sliced specimen with respect to the specimen stage and a specimen stage control means controlling the specimen stage based on a specimen posture recognized by the posture recognition means and an installation angle of the gas-ion beam irradiation apparatus in order to allow an incident angle of the gas-ion beam with respect to the obverse or the reverse of the sliced specimen to be a desired value.

Abstract: A sample to be observed or processed with a focused charged particle beam is formed small, and only the micro sample is locally cooled. Alternatively, a sample mount part is used which has the structure that can relax a thermal drift.

Abstract: A composition containing an extremely poorly water-soluble drug and obtained by treating, with a supercritical fluid or subcritical fluid of carbon dioxide, a mixture comprising a porous silica material and the extremely poorly water-soluble drug; and its production process. The porous silica material has an average pore diameter in a range of from 1 to 20 nm, pores having diameters within ±40% of the average pore size account for at least 60% of a total pore volume of the porous silica material, and in X-ray diffractometry, the porous silica material has at least one peak at a position of diffraction angle (2?) corresponding to a d value of at least 1 nm. The composition according to the present invention, which contains the extremely poorly water-soluble drug, is excellent in the dissolution of the extremely poorly water-soluble drug.

Abstract: A method for optically immobilizing very small objects, where a material with the capability of photoinduced deformation to possibly immobilize very small objects is used at least as a surface layer of a carrier and where the very small objects are immobilized via irradiation light while they are arranged on the surface of the carrier. Very small objects particularly preferably include proteins, nucleic acids and the like. A very small object-immobilized carrier having immobilized very small objects in such manner, particularly a biosensor. A method for observing a very small object immobilized on the surface of a carrier by an appropriate approach giving displacement force to the very small object. The present invention provides a method for strongly immobilizing very small objects on the surface of a carrier with a simple tool.

Abstract: To provide a method of controlling film thickness of dielectric multilayer film, such as optical thin film, with high precision, an optical film thickness controlling apparatus and a dielectric multilayer film manufacturing apparatus that can control the film thickness based on the same method, and dielectric multilayer film manufactured using the controlling apparatus or manufacturing apparatus. An optical film thickness controlling apparatus includes a film formation device 15 having a rotatable substrate 23 and a sputtering target 28, a photodiode 16 that detects each of a plurality of monochromatic light beams applied to the rotatable substrate along a radius thereof at predetermined intervals, and an A/D converter 17, in which a movable shutter 29 that moves along the direction of the radius of the rotatable substrate 23 to shut off film formation on the substrate 23 is provided between the substrate 23 and the target 28.

Abstract: The surface of oxidase is stabilized by stabilizing the inner structure responsible for the enzyme function of the oxidase via the alteration of unstable amino acids therein into stable amino acids, and/or immobilizing the oxidase in a structure unit with structural stability and of a given inner diameter. The inner structure of oxidase is stabilized by altering specific unstable amino acids positioned within a 12-angstrom radius range from the active center of the oxidase or altering specific unstable amino acids facing the hydrogen peroxide-binding pocket of the oxidase. The process enhances the stability of the oxidase, particularly the stability of oxidase with lignin degradation activity against hydrogen peroxide.

Abstract: A method for protein synthesis for efficiently preparing a protein with intermolecular and/or intramolecular disulfide bonds synthetically, using a cell-free extract solution to which a template gene is added. The protein with intermolecular and/or intramolecular disulfide bonds is preferably an antibody or a metalloprotein. The method more preferably includes adding a protein with the chaperone function to the cell-free extract solution and/or eliminating reducing agents from the cell-free extract solution. A method for protein screening and a retrieval method for protein function, using this method for protein synthesis. The method provides a method for protein synthesis capable of efficiently preparing a protein with intermolecular and/or intramolecular disulfide bonds synthetically by cell-free systems (in vitro transcription/translations systems).

Abstract: In order provide an energy dispersive X-ray analyzer that can perform efficient measurement by optimizing signal processing time of an X-ray counting section, an energy dispersive X-ray analyzer is provided with an energy dispersive X-ray detector, an X-ray counting section for analyzing the signal from the energy dispersive X-ray detector and generating a frequency distribution for each energy level, namely an energy spectrum, and a data control section for performing data processing, user interface etc. The X-ray counting section of the energy dispersive X-ray analyzer has signal processing methods with different processing times for a single X-ray input, and a function for selecting which method result to make use of using input X-ray energy.

Abstract: The invention features an immobilized enzyme wherein an enzyme or an active unit of the enzyme is immobilized in a structural unit having structural stability; namely, the present invention provides an immobilized enzyme comprising: a structural unit having structural stability; and an enzyme having an active unit, the enzyme, or the active unit being immobilized in the structural unit; in the preferred aspects, the structural unit is a porous substance having homogeneous pores, a pore size (diameter) of the porous substance of the structural unit is almost the same as that of the enzyme to be immobilized or the active unit of the enzyme, and the structural unit comprises a mesoporous silica porous material to be formed via a layered silicate.

Abstract: In order provide an energy dispersive X-ray analyzer that can perform efficient measurement by optimizing signal processing time of an X-ray counting section, an energy dispersive X-ray analyzer is provided with an energy dispersive X-ray detector, an X-ray counting section for analyzing the signal from the energy dispersive X-ray detector and generating a frequency distribution for each energy level, namely an energy spectrum, and a data control section for performing data processing, user interface etc. The X-ray counting section of the energy dispersive X-ray analyzer has signal processing methods with different processing times for a single X-ray input, and a function for selecting which method result to make use of using input X-ray energy.

Abstract: A deflection current/high voltage integration type power supply has a flyback transformer having a primary winding and a secondary winding; a series circuit of a deflection coil and a first capacitor, connected in series to the primary winding of the flyback transformer; a resonance capacitor connected in parallel to the series circuit of the deflection coil and the first capacitor; a first switching element, connected in parallel with the series circuit of the deflection coil and the first capacitor, to be turned on/turned off by a drive signal so that a high voltage is generated at the secondary winding of the flyback transformer and a deflection current flows in the deflection coil; a parallel connection circuit of a second switching element and a second capacitor, connected in series to the primary winding of the flyback transformer; and a switching control means to control the deflection current flowing in the deflection coil and the high voltage generated at the secondary winding of the flyback transfor

Abstract: Display is made of the spectrums of the fluorescent X-rays by using the ordinate axis as representing the square root of a fluorescent X-ray intensity and using the abscissa axis as representing the energy of the fluorescent X-rays.

Abstract: A high-voltage generating circuit includes a high-voltage production circuit, a high-voltage detecting circuit, and a control circuit. The high-voltage detecting circuit has a high-voltage circuit section to which a voltage of 1 kV to several tens of kilovolts is applied and a low-voltage circuit section to which a voltage of several tens of volts is applied. In the high-voltage circuit section, a parallel circuit formed of a first voltage-dividing resistor and a part of a second voltage-dividing resistor and a speed-up capacitor, and another parallel circuit formed of the remaining part of the second voltage-dividing resistor and a third voltage-dividing resistor and another speed-up capacitor are connected in series to form a two-stage parallel circuit.

Abstract: A high-voltage generating circuit includes a high-voltage production circuit, a high-voltage detecting circuit and a control circuit. The high-voltage production circuit includes clamping diodes to provide a good response for stabilizing a high voltage. The control circuit applies pulse-width control to a power source voltage. The high-voltage detecting circuit has a high-voltage circuit section to which a voltage of 1 kV to several tens of kilovolts is applied and a low-voltage circuit section to which a voltage of several tens of volts is applied. The high-voltage circuit section comprises a parallel circuit including voltage-dividing resistors and a speed-up capacitor. The speed-up capacitor is set to 1000 pF or less.

Abstract: An optical filter having a transmission wavelength to be tuned dependent on light incident angle is rotatively provided in a collimated light path. The optical filter is rotated by the displacement of a piezoelectric actuator by a structure in which the displacement is converted to the rotation of the optical filter. The converting structure comprises a displacement magnifying mechanism for magnifying the displacement to provide the magnified displacement and a motion converting mechanism for converting the magnified displacement to the rotation of the optical filter.

Abstract: Process for the preparation of motilin-like polypeptide, a recombinant DNA therefor, and a plasmid, in which the recombinant DNA is inserted.

Abstract: A readily absorbable type of motilin preparation containing a motilin-like active substance and a surface active agent is provided, in which the motilin-like active substance is mainly L-leucine-13-motilin-homoserine, and the surface active agent is selected from the group consisting of a bile salt, saponin and a polyethylene glycol higher-alcohol ether for nasal administration purposes and is a peptide lytic enzyme inhibitor for oral administration purposes.

Abstract: A process for the preparation of a polypeptide of the formula ##STR1## wherein A' is Pro, Gly, Asp or Ser; B' is Gly, Asn or Ser; C is Gln, Glu or Asp; D is Asn, Glu or Asp; X' is a residue of amino acid other than Met; E is Gln, Lys or Arg, and Y' is a residue of homoserine or homoserine-lactone, as well as a recombinant DNA and expression plasmid for preparing the polypeptide.

Abstract: A pharmaceutical composition for stimulating peristaltic contraction in the intestinal canal which contains a pharmaceutically acceptable carrier or excipient and an effective amount of a polypeptide, or a salt thereof, having a pharmacological activity similar to motilin. The polypeptide has the formula B.sub.1 -Z.sub.1 -A.sub.1 -Z.sub.2 -B.sub.2 -Thr-B.sub.3 -A.sub.2 -Glu-Z.sub.3 -C-Arg-X'-Gln-Glu-Lys-Glu-Arg-D-Lys-Gly-E-Y, with Z.sub.1,Z.sub.2 and Z.sub.3 each being an amino acid residue having a hydrophobic side-chain, A.sub.1 and A.sub.2 each being an amino acid residue such as Pro, Gly, Asn and Ser, B.sub.1, B.sub.2 and B.sub.