Patents by Inventor Hatim T. Allawi

Hatim T. Allawi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR ANALYSIS OF NUCLEIC ACID MOLECULES DURING AMPLIFICATION REACTIONS
    Publication number: 20140057259
    Abstract: The present invention provides systems, methods and kits for performing a detection assay (e.g., invasive cleavage assay) in combination with an amplification assay (e.g., PCR), where the detection assay employs enzyme footprint probes with relatively short (e.g., 6-12 bases) analyte-specific regions configured to provide a preferred footprint length of duplex for use with a particular nucleic acid modifying enzyme. In some embodiments, such assays are used for target quantification, and in other embodiments, such assays are used for genotyping. In certain embodiments, the use of such short probes allows for assays with increased dynamic range.
    Type: Application
    Filed: July 12, 2013
    Publication date: February 27, 2014
    Applicant: Hologic, Inc.
    Inventors: Hatim T. Allawi, Victor I. Lyamichev
  • Detection of small nucleic acids
    Patent number: 8609336
    Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: December 17, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Hatim T Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn Olson-Munoz, LuAnne Chehak, Sarah M. Olson
  • DETECTION OF NUCLEIC ACIDS
    Publication number: 20120231461
    Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
    Type: Application
    Filed: May 24, 2012
    Publication date: September 13, 2012
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Hatim T. Allawi, Victor Lyamichev
  • Detection of nucleic acids
    Patent number: 8206904
    Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: June 26, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Victor Lyamichev
  • Detection of Small Nucleic Acids
    Publication number: 20120149018
    Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
    Type: Application
    Filed: February 7, 2012
    Publication date: June 14, 2012
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: James E. Dahlberg, Hatim T. Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn C. Olson-Munoz, LuAnne Chehak, Sarah M. Olson
  • Methods and applications for target quantification
    Patent number: 8190371
    Abstract: The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.
    Type: Grant
    Filed: September 8, 2008
    Date of Patent: May 29, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Victor Lyamichev, Rex Piper
  • RNA detection assays
    Patent number: 7935800
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: December 19, 2006
    Date of Patent: May 3, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Detection of small nucleic acids
    Patent number: 7851150
    Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
    Type: Grant
    Filed: December 18, 2003
    Date of Patent: December 14, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Hatim T. Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn C. Olson-Munoz, LuAnne Chehak, Sarah M. Olson
  • RNA detection assays
    Patent number: 7807806
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: October 5, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, Wupo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Charge tags and the separation of nucleic acid molecules
    Patent number: 7678542
    Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
    Type: Grant
    Filed: June 3, 2008
    Date of Patent: March 16, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Y. Takova, Bruce P. Neri, James R. Prudent, Jeff G. Hall
  • METHODS AND APPLICATIONS FOR TARGET QUANTIFICATION
    Publication number: 20090299641
    Abstract: The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.
    Type: Application
    Filed: September 8, 2008
    Publication date: December 3, 2009
    Applicant: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Victor Lyamichev, Rex Piper
  • COMPOSITIONS AND METHODS FOR ANALYSIS OF NUCLEIC ACID MOLECULES DURING AMPLIFICATION REACTIONS
    Publication number: 20090253142
    Abstract: The present invention provides systems, methods and kits for performing a detection assay (e.g., invasive cleavage assay) in combination with an amplification assay (e.g., PCR), where the detection assay employs enzyme footprint probes with relatively short (e.g., 6-12 bases) analyte-specific regions configured to provide a preferred footprint length of duplex for use with a particular nucleic acid modifying enzyme. In some embodiments, such assays are used for target quantification, and in other embodiments, such assays are used for genotyping. In certain embodiments, the use of such short probes allows for assays with increased dynamic range.
    Type: Application
    Filed: March 13, 2009
    Publication date: October 8, 2009
    Applicant: HOLOGIC, INC.
    Inventors: Hatim T. Allawi, Victor I. Lyamichev
  • RNA Detection Assays
    Publication number: 20090142754
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Application
    Filed: October 30, 2007
    Publication date: June 4, 2009
    Applicant: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • CHARGE TAGS AND THE SEPARATION OF NUCLEIC ACID MOLECULES
    Publication number: 20090078574
    Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
    Type: Application
    Filed: June 3, 2008
    Publication date: March 26, 2009
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Y. Takova, Bruce P. Neri, James R. Prudent, Jeff G. Hall
  • Endonuclease-substrate complexes
    Patent number: 7482118
    Abstract: The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: February 20, 2004
    Date of Patent: January 27, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Michael W. Kaiser, Wu-Po Ma, Bruce P. Neri, Victor Lyamichev
  • Detection of Small Nucleic Acids
    Publication number: 20080187926
    Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
    Type: Application
    Filed: October 30, 2007
    Publication date: August 7, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: James E. Dahlberg, Hatim T. Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn C. Olson-Munoz, LuAnne Chehak, Sarah M. Olson
  • Charge tags and the separation of nucleic acid molecules
    Patent number: 7384746
    Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
    Type: Grant
    Filed: June 23, 2004
    Date of Patent: June 10, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Y. Takova, Bruce P. Neri, James R. Prudent, Jeff G. Hall
  • Detection of nucleic acids
    Publication number: 20080131890
    Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
    Type: Application
    Filed: June 1, 2007
    Publication date: June 5, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Victor Lyamichev
  • RNA detection assays
    Patent number: 7150982
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: February 26, 2002
    Date of Patent: December 19, 2006
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Charge tags and the separation of nucleic acid molecules
    Patent number: 6780982
    Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
    Type: Grant
    Filed: February 6, 2001
    Date of Patent: August 24, 2004
    Assignee: Third Wave Technologies, Inc.
    Inventors: Victor Lyamichev, Zbigniew Skrzpczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Takova, Bruce P. Neri