Abstract: The present invention provides for a liver tissue model construct composed of biomaterials and cells, to be used for scientific research within in the 3D liver tissue modelling field. The applications of said tissue model construct can be specific for pharmaceutical evaluations and/or discoveries, regenerative medicine investigations, tissue engineering developments, and liver physiology and/or pathology.

Abstract: The present disclosure relates to the field of liquid handling and dispensing systems in combination with additive manufacturing. In particular, it relates to temperature-controlled units, i.e. dispensing heads and source well holders, for receiving, holding and releasing liquid and semi-liquid material, liquid-handling and dispensing systems, apparatuses and methods for applying temperature-sensitive liquids. A temperature-controlled unit (1) comprises at least one Peltier element (3), each Peltier element having opposite first and second surfaces (4a, 4b). The unit (1) further comprises at least one cooling element (5). The at least one Peltier element (3) is arranged to have each respective first surface (4a) facing a reservoir block (2) of the unit (1). The at least one cooling element (5) is thermally connected to the Peltier element (3) and arranged to transfer heat generated by the at least one Peltier element (3) and dissipate the transferred heat away from the at least one Peltier element (3).

Abstract: A three-dimensional (3D) bioprinting method and system are disclosed. The method includes disposing/immersing a printing platform or surface into a first bioink, such as a bioink resin, curing one or more layer of the first bioink resin onto the printing platform or surface, and removing the printing platform or surface from the first bioink resin. The process is repeated with a second bioink resin such that the second bioink resin is cured on top of the one or more layer of first bioink resin, and can be further repeated with a third or even fourth bioink resin. By varying constituents of one or more or each bioink resin (such as living cell type or polymer), complex, multilayered tissues can be engineered. A system capable of performing the method is also disclosed.

Abstract: The present invention relates to a cell monitoring system for automatic cell monitoring and to a compact fluorescence microscope for high resolution live-cell imaging. The microscope comprises light sources (12), light source lenses (11), excitation filters (10), and beam combining means (9). The detector unit comprises emission filter (4), tube lens (5), and a detector (7) and optionally beam folding elements (6) for compactness. A dielectric mirror (3) combines and divides the excitation and the emission light.

Abstract: A material cartridge arrangement (100, 101) for a dispensing system, comprising a material cartridge (2) having a first end (2a), an opposing second end (2b) and an ink material channel (5) extending between the first and second ends in the material cartridge (2), the ink material channel (5) being bounded by an ink material channel wall/walls (9) extending between the first (2a) and second ends (2b). An ink material pressurising device (3) is arranged to cause ink material hold in the ink material channel (5) to flow in a direction from said first end (2a) towards said second end (2b) and through an ink material outlet. At least one temperature sensing element (16) is arranged at a position along the direction of extension of the ink material channel (5) and arranged to measure a temperature at the ink material channel wall (9).

Abstract: A perfusion device for printing a 3D construct with internal channels is disclosed. The device comprises a housing with an internal chamber, the chamber configured to hold the construct, and having a bottom plate configured to support the construct, the chamber comprising at least one inlet or outlet configured to introduce a liquid from outside the chamber into the chamber or vice versa. The at least one inlet or outlet comprises an connector adapted to connect or align the one or more internal channels within the 3D construct to the inlet or outlet when the 3D construct is present in the chamber. Further a method for printing a 3D construct with at least one internal channel is disclosed.

Abstract: The present invention relates to a double network bioink that is customizable and tailorable in terms of mechanical characteristics, diffusivity, and biological functionality that is printable at room temperature and cytocompatible. Double network bioinks of the present invention can be utilized as a standard for comparison and evaluation of bioinks and during calibration and/or are suited for the education field as a “blank slate” bioink that can be utilized to convey different scientific concepts. Specific bioinks included comprise: one or more biocompatible or non-biocompatible thickener; one or more polyethylene glycol based crosslinkable network; one or more photoinitiator; and/or optionally, one or more additives to impart desired and/or different characteristics to the bioink. In embodiments the thickener and polyethylene glycol based crosslinkable network form a structure comprising two interpenetrating networks.

Abstract: A dispensing system (1) comprising a material cartridge (2) provided with a nozzle (3), a temperature-regulating unit (4) provided with a nozzle temperature-regulating zone (5) arranged to regulate the temperature of the material cartridge nozzle (3) when the material cartridge nozzle (3) is arranged within the nozzle temperature-regulating zone (5), and a positioning unit (6) arranged for movement of the material cartridge (2) and the nozzle temperature-regulating zone (5) relative to each other between a first position (A) and a second position (B). In the first position (A) the material cartridge nozzle (3) is arranged within the nozzle temperature-regulating zone (5), and in the second position (B) at least a leading end portion (3a) of the material cartridge nozzle (3) is arranged outside the nozzle temperature-regulating zone (5).

Abstract: The present disclosure relates to a print bed (1) for regulating a temperature of the print bed (1). The print bed (1) comprises at least one Peltier element (2), each Peltier element having opposite first and a second surfaces (3a, 3b). The print bed (1) further comprises at least one heatsink (4). The at least one Peltier element (2) is arranged to have each respective first surface (3a) facing a print surface (5) of the print bed (1). The at least one heatsink (4) is thermally connected to the Peltier element (2) and arranged to transfer heat generated by the at least one Peltier element (2) and dissipate the transferred heat away from the at least one Peltier element (2). The present disclosure further relates to corresponding 3D-printers, methods, computer programs and modules.

Abstract: The present invention relates to a double network bioink that is customizable and tailorable in terms of mechanical characteristics, diffusivity, and biological functionality that is printable at room temperature and cytocompatible. Double network bioinks of the present invention can be utilized as a standard for comparison and evaluation of bioinks and during calibration and/or are suited for the education field as a “blank slate” bioink that can be utilized to convey different scientific concepts. Specific bioinks included comprise: one or more biocompatible or non-biocompatible thickener; one or more polyethylene glycol based crosslinkable network; one or more photoinitiator; and/or optionally, one or more additives to impart desired and/or different characteristics to the bioink. In embodiments the thickener and polyethylene glycol based crosslinkable network form a structure comprising two interpenetrating networks.

Abstract: The present disclosure relates to a a computer implemented method for determining a substance dispensing plan. The method comprises obtaining (S10) a digital representation of a set of a person's current bodily features. The method further comprises determining (S20) a substance dispensing plan based on a comparison between the digital representation of the set of the person's current bodily features and a set of desired bodily features. The present disclosure also relates to corresponding computer programs, control systems and robotic systems.

Abstract: A three-dimensional (3D) bioprinting method and system are disclosed. The method includes disposing/immersing a printing platform or surface into a first bioink, such as a bioink resin, curing one or more layer of the first bioink resin onto the printing platform or surface, and removing the printing platform or surface from the first bioink resin. The process is repeated with a second bioink resin such that the second bioink resin is cured on top of the one or more layer of first bioink resin, and can be further repeated with a third or even fourth bioink resin. By varying constituents of one or more or each bioink resin (such as living cell type or polymer), complex, multilayered tissues can be engineered. A system capable of performing the method is also disclosed.

Abstract: Systems and methods for optical assessments of bioink printability are described. The systems and methods include the use of hardware, software and optical targets to aid in the evaluation of bioink printability. The optical targets are developed and fabricated from 3D printable materials determined to be “nozzle fidelic” and/or materials that possess well characterized thermosensitivity. The optical targets make it possible to rapidly compare and evaluate bioink printability and can be easily customized and tailored for specific applications.

Abstract: The present disclosure relates to a method for arranging cellular material in a bioink, gel or hydrogel material used in 3D-bioprinting. The method comprises bioprinting or dispensing at least one layer of bioink/gel/hydrogel, dispensing or patterning cellular material in the form of single cells, spheroids or cell suspension on or in the bioink/gel/hydrogel layer using a microfluidic device, and repeating previous steps in order to create a 3D tissue model with multiple cell layers. The present disclosure also relates to corresponding microfluidic devices, computer programs and 3D bioprinters.

Abstract: Bioink compositions comprising a biomaterial (mammalian, plant based, synthetically derived, or microbially derived) such as a hydrogel and a microbial-, fungal-, or plant-produced polysaccharide, with or without cells, for use in the 3D bioprinting of human tissues and scaffolds are described. The bioink compositions have excellent printability and improved cell function, viability and engraftment. Furthermore, the bioink compositions can be supplemented through the additional of auxiliary proteins and other molecules such as growth factors including extracellular matrix components, Laminins, super affinity growth factors and morphogens. The bioink compositions can be used under physiological conditions related to 3D bioprinting parameters which are cytocompatible (e.g. temperature, printing pressure, nozzle size, bioink gelation process).

Abstract: The present disclosure relates to a bioprinter system comprising a printbed, at least one of exchangeable and/or fixed toolhead, a cell culture monitor device, the device comprising a camera arranged to provide images of a cell culture or construct at the printbed, and a processing element arranged to monitor cell status at the printbed based on the provided images.

Abstract: The present disclosure relates to a 3D bioprinter (1) comprising a base unit (2). The base unit (2) has a support (3) adapted for mounting of at least one toolhead (4), a communication interface part (5) for communication of data with the at least one toolhead (4), when mounted, and a base unit processing element (7) adapted to communicate with a toolhead processing element (8) of the at least one toolhead over said communication interface part (5). The present disclosure relates further to a 3D bioprinter toolhead.

Abstract: The present disclosure relates to systems and methods for analyzing citationally related content and identifying, based on the analysis, a risk of impliedly overruled content. Embodiments provide for receiving case law data from a document source, for extracting a case triple that includes a first case overruling or abrogating a second case, and a third case citationally related to the second case. Features may be generated from case triple, such as natural processing language features comparing the language in the various cases of the triple, and feeding the generated features to a main classifier. In embodiments, the main classifier classifies the case triple into a class indicating the risk probability that the second case is impliedly overruled by the first case.

Abstract: The present invention relates to preparation of bioink composed of cellulose nanofibril hydrogel with native or synthetic Calcium containing particles. The concentration of the calcium containing particles can be between 1% and 40% w/v. Such bioink can be 3D Bioprinted with or without human or animal cells. Coaxial needle can be used where cellulose nanofibril hydrogel filled with Calcium particles can be used as shell and another hydrogel based bioink mixed with cells can be used as core or opposite. Such 3D Bioprinted constructs exhibit high porosity due to shear thinning properties of cellulose nanofibrils which provides excellent printing fidelity. They also have excellent mechanical properties and are easily handled as large constructs for patient-specific bone cavities which need to be repaired. The porosity promotes vascularization which is crucial for oxygen and nutrient supply. The porosity also makes it possible for further recruitment of cells which accelerate bone healing process.

Abstract: A method of working a gas turbine engine component, for example, a turbine airfoil platform having a heat crack, is disclosed. The presented method may allow turbine airfoils to be repaired, modified, manufactured or otherwise worked and subsequently assembled into a turbine section of a gas turbine engine. This method may help to reduce the costs of operating a gas turbine engine by allowing damaged turbine airfoils to be repaired rather than discarded.