Patents by Inventor Helmut Merk
Helmut Merk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210071220Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: April 3, 2020Publication date: March 11, 2021Inventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20190382818Abstract: The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3? and 5? ends with an adapter primer; b) the product from step a) is hybridized on the 3? and 5? ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3? and 5? ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.Type: ApplicationFiled: January 18, 2019Publication date: December 19, 2019Inventors: Helmut Merk, Volker Erdmann, Wolfgang Stiege
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Publication number: 20190300924Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: May 16, 2019Publication date: October 3, 2019Inventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20170327859Abstract: The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3? and 5? ends with an adapter primer; b) the product from step a) is hybridized on the 3? and 5? ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3? and 5? ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.Type: ApplicationFiled: December 22, 2016Publication date: November 16, 2017Inventors: Helmut Merk, Volker Erdmann, Wolfgang Stiege
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Publication number: 20170198327Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: March 27, 2017Publication date: July 13, 2017Inventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20170029828Abstract: The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optionally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.Type: ApplicationFiled: October 13, 2016Publication date: February 2, 2017Inventors: Helmut Merk, Wolfgang Stiege
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Publication number: 20160177320Abstract: The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optionally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.Type: ApplicationFiled: July 21, 2015Publication date: June 23, 2016Inventors: Helmut Merk, Wolfgang Stiege
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Publication number: 20160115514Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: June 2, 2015Publication date: April 28, 2016Inventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20150368686Abstract: The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3? and 5? ends with an adapter primer; b) the product from step a) is hybridized on the 3? and 5? ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3? and 5? ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.Type: ApplicationFiled: January 26, 2015Publication date: December 24, 2015Inventors: Helmut Merk, Volker Erdmann, Wolfgang Stiege
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Publication number: 20150079629Abstract: The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optionally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.Type: ApplicationFiled: November 24, 2014Publication date: March 19, 2015Inventors: Helmut Merk, Wolfgang Stiege
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Patent number: 8828670Abstract: The invention relates to a method for producing monomeric or dimeric proteins or peptides containing internal or external disulfide bonds, comprising the following steps: a) a cell-free lysate, obtainable from eukaryotic cells, is provided, which contains functional microsomal vesicles, b) a nucleic acid coding the protein or peptide and additionally containing a signal sequence is added to the lysate, c) the lysate with the nucleic acid is held for a given time at a temperature in the range from 20 to 35° C., proteins or peptides formed with the nucleic acid being translocated into the microsomal vesicles, d) the microsomal vesicles are then dissolved, and the proteins or peptides obtained thereby are optionally separated from the lysate.Type: GrantFiled: July 10, 2012Date of Patent: September 9, 2014Assignee: RiNA Netzwerk RNA Technologien GmbHInventors: Helmut Merk, Wolfgang Stiege, Christine Gless, Michael Gerrits
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Publication number: 20140162313Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: January 10, 2014Publication date: June 12, 2014Inventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20140120576Abstract: The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optionally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.Type: ApplicationFiled: June 20, 2013Publication date: May 1, 2014Applicant: SIEMENS BUILDING TECHNOLOGIES AGInventors: Helmut Merk, Wolfgang Stiege
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Publication number: 20130261290Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: April 19, 2013Publication date: October 3, 2013Inventors: JAN STREY, HELMUT MERK, WOLFGANG STIEGE
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Publication number: 20130017576Abstract: The invention relates to a method for producing monomeric or dimeric proteins or peptides containing internal or external disulfide bonds, comprising the following steps: a) a cell-free lysate, obtainable from eukaryotic cells, is provided, which contains functional microsomal vesicles, b) a nucleic acid coding the protein or peptide and additionally containing a signal sequence is added to the lysate, c) the lysate with the nucleic acid is held for a given time at a temperature in the range from 20 to 35° C., proteins or peptides formed with the nucleic acid being translocated into the microsomal vesicles, d) the microsomal vesicles are then dissolved, and the proteins or peptides obtained thereby are optionally separated from the lysate.Type: ApplicationFiled: July 10, 2012Publication date: January 17, 2013Applicant: RINA NETZWERK RNA TECHNLOGIEN GMBHInventors: Helmut Merk, Wolfgang Stiege, Christine Gless, Michael GERRITS
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Publication number: 20120315669Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).Type: ApplicationFiled: April 10, 2012Publication date: December 13, 2012Applicant: RINA-NETZWERK RNA TECHNOLOGIEN GMBHInventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20120142048Abstract: The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optimally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.Type: ApplicationFiled: September 1, 2011Publication date: June 7, 2012Applicant: SIEMENS BUILDING TECHNOLOGIES AGInventors: Helmut Merk, Wolfgang Stiege
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Publication number: 20110212452Abstract: The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3? and 5? ends with an adapter primer; b) the product from step a) is hybridized on the 3? and 5? ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3? and 5? ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.Type: ApplicationFiled: February 4, 2009Publication date: September 1, 2011Applicant: RiNA-NETZWRK RNA-Technologien GmbHInventors: Helmut Merk, Vlker Erdmann, Wolfgang Stiege
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Publication number: 20100196952Abstract: The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, without separating generated substances and without adding consumed synthesis substances within the defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products and/or reaction inhibitors are separated from the solution (and extracted), d) immediately before, after or at the same time as step c) consumed synthesis substancesType: ApplicationFiled: January 8, 2010Publication date: August 5, 2010Applicant: RINA-NETZWERK RNA TECHNOLOGIEN GMBHInventors: Jan Strey, Helmut Merk, Wolfgang Stiege
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Publication number: 20080096256Abstract: The invention relates to a method for gene expression in a cell-free translation system, wherein the reaction solution comprises an RNA matrix with a gene sequence, which codes for an expression product to be expressed, and a translation system from eukaryotic cells, wherein the reaction solution is incubated, and wherein the expression product is optionally separated from the reaction solution, characterized by that the RNA matrix, viewed in the 5?-3? direction, comprises a Shine Dalgarno sequence, connected to a first spacer sequence and connected to the gene sequence.Type: ApplicationFiled: March 30, 2007Publication date: April 24, 2008Inventors: Helmut Merk, Wolfgang Stiege, Stefan Kubick