Abstract: The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3? and 5? ends with an adapter primer; b) the product from step a) is hybridized on the 3? and 5? ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3? and 5? ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.

Abstract: The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, without separating generated substances and without adding consumed synthesis substances within the defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products and/or reaction inhibitors are separated from the solution (and extracted), d) immediately before, after or at the same time as step c) consumed synthesis substances

Abstract: The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optionally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B.

Abstract: A filter holder assembly is provided for the optional holding in a motor vehicle at a dust filter alone or a dust filter and an absorption filter which is optionally arranged directly downstream of the dust filter. A frame lower part is provided which either holds the dust filter or the absorption filter. When the frame lower part holds the absorption filter, a frame upper part which holds the dust filter is releasably arranged upstream of the frame lower filter.

Abstract: In a filter insert which is received exchangeably in a shaft along a channel and which can be fixed sealingly relative to frame shaped shaft walls via at least one gasket preferably continuous on a flow-off side of the filter, it becomes possible to change the filter easily, without the danger of damaging the gasket, if there is at least one pressing element which can be connected to a pressure reducing device. When the pressing element is in the contracted pressure reduced position, a continuous gap forms between the gasket and associated shaft wall during a shift of the filter, this gap being cancelled when the pressure reducing device is disconnected.

Abstract: A connecting element for connecting a fluid line to a component which can be acted upon fluidically, comprising a C-shaped retaining clamp and a bore which passes through at an angle to the retaining clamp is attached to a connection bore of the component in such a way that a push-in connection piece of the fluid line, which is pressed into the retaining clamp transversely to its longitudinal axis, is at the same time inserted through the bore and into the connection bore for forming a fluidic push-in connection.