Patents by Inventor Hideki Kambara

Hideki Kambara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11214796
    Abstract: The present invention provides a method and/or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: January 4, 2022
    Assignee: HITACHI, LTD.
    Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi
  • Publication number: 20210388422
    Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.
    Type: Application
    Filed: May 18, 2021
    Publication date: December 16, 2021
    Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI, Maiko TANABE
  • Patent number: 11053536
    Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.
    Type: Grant
    Filed: July 30, 2012
    Date of Patent: July 6, 2021
    Assignee: Hitachi, Ltd.
    Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
  • Publication number: 20180238775
    Abstract: The purpose of the present invention is to provide a sample collection system capable of repeatedly collecting a sample. The present invention discloses a system including: at least one or multiple hollow collection needle(s) each having a knife edge for collecting the sample from a biological specimen; and a syringe, a solenoid and a reservoir that serve as a liquid-inflow mechanism allowing a solution to flow into the collection needle, wherein the sample is ejected from the collection needle together with the solution so as to recover the sample.
    Type: Application
    Filed: March 30, 2018
    Publication date: August 23, 2018
    Applicant: FRONTIER BIO SYSTEMS, INC.
    Inventors: Hideki KAMBARA, Masahito HOSOKAWA, Haruko TAKEYAMA, Takuya YODA
  • Patent number: 10030240
    Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: July 24, 2018
    Assignee: Hitachi, Ltd.
    Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
  • Patent number: 9862993
    Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a genetic analysis system, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: January 9, 2018
    Assignee: HITACHI, LTD.
    Inventors: Maiko Tanabe, Hideki Kambara, Masataka Shirai
  • Publication number: 20170283796
    Abstract: The present invention provides a method and/or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.
    Type: Application
    Filed: June 13, 2017
    Publication date: October 5, 2017
    Applicant: HITACHI, LTD.
    Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi
  • Patent number: 9708603
    Abstract: The problem to be solved by the present invention is to provide a method for preparing a sample for comprehensively and accurately analyzing gene expression in a single cell or a few cells, for example, by a large-scale DNA sequencer.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: July 18, 2017
    Assignee: HITACHI, LTD.
    Inventors: Hiroyuki Tsunoda, Huan Huang, Mari Ohta, Hideki Kambara
  • Publication number: 20170016814
    Abstract: An object is to provide a biomolecule analyzer capable of collecting and analyzing a biomolecule in a single cell without damaging neighboring cells. In order to achieve this object, a biomolecule analyzer according to the present invention is characterized by including a unit which obtains an optical image of a plurality of cells, a unit which disrupts a part or the whole of at least one cell of the plurality of cells, an array device in which regions for capturing a biomolecule in the cell released by the disrupting unit are arranged, and a unit which associates the region in which the biomolecule is captured in the array device with a portion corresponding to the cell disrupted by the disrupting unit in the optical image.
    Type: Application
    Filed: December 27, 2013
    Publication date: January 19, 2017
    Applicant: HITACHI, LTD.
    Inventors: Masataka Shirai, Hideki Kambara, Tomoharu Kajiyama
  • Publication number: 20160333397
    Abstract: A reaction liquid after a nucleic acid amplification reaction is made suitable for various processes. A step of measuring the amount of a target product and the amount of a byproduct after performing a nucleic acid amplification reaction, and a step of determining that a process for removing the byproduct is needed when the abundance ratio of the target product to the byproduct is lower than a prescribed value, and determining the dilution ratio of a reaction liquid after the nucleic acid amplification reaction when the abundance ratio is higher than the prescribed value are included.
    Type: Application
    Filed: January 27, 2014
    Publication date: November 17, 2016
    Applicant: HITACHI, LTD.
    Inventors: Hiroko Matsunaga, Tomoharu Kajiyama, Mari Ohta, Hideki Kambara
  • Publication number: 20160251705
    Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a means, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.
    Type: Application
    Filed: October 21, 2013
    Publication date: September 1, 2016
    Applicant: HITACHI, LTD.
    Inventors: Maiko Tanabe, Hideki Kambara, Masataka Shirai
  • Patent number: 9428795
    Abstract: To provide a means and a method of quickly sampling a tissue fragment from a plant tissue, quickly preserving a gene expression state within the tissue fragment, and comprehensively analyzing the gene expression state. The present invention relates to a method of sampling a plant tissue section, comprising: inserting a first gel layer into a needle; arranging a plant tissue on a second gel layer; and passing the needle through the plant tissue together with the second gel layer, and sampling a section of the plant tissue in the needle.
    Type: Grant
    Filed: December 18, 2012
    Date of Patent: August 30, 2016
    Assignee: Hitachi, Ltd.
    Inventors: Tomoharu Kajiyama, Hideki Kambara, Toru Habu
  • Patent number: 9278355
    Abstract: This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.
    Type: Grant
    Filed: November 13, 2008
    Date of Patent: March 8, 2016
    Assignee: Hitachi, Ltd.
    Inventors: Tomoharu Kajiyama, Masataka Shirai, Hideki Kambara
  • Publication number: 20160010078
    Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.
    Type: Application
    Filed: March 12, 2013
    Publication date: January 14, 2016
    Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI, Maiko TANABE
  • Publication number: 20150302042
    Abstract: The present invention aims to provide a data analysis apparatus capable of clustering appropriately even when there is an exceptional datum resulted from an experimental error and the like. In the data analysis apparatus according to the invention, a cluster range parameter for stretching a cluster boundary is determined in advance according to the range of an experimental error which an experimental error datum describes. In the process of clustering, an exceptional datum which does not belong to any cluster is determined to belong to a cluster when an area at a distance determined by the cluster range parameter from the exceptional datum is contained in the cluster, and the exceptional datum is determined to form an independent cluster when even the area at the distance is not contained in any cluster (see FIG. 7).
    Type: Application
    Filed: November 20, 2012
    Publication date: October 22, 2015
    Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI
  • Publication number: 20150167063
    Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.
    Type: Application
    Filed: July 30, 2012
    Publication date: June 18, 2015
    Applicant: Hitachi, Ltd.
    Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
  • Publication number: 20150159202
    Abstract: A method of synthesizing cDNA, in sequence, includes picking up a whole single-cell from a sample containing at least a single-cell having a cell membrane, lysing the cell membrane and extracting nucleic acids from the cell, degrading DNA of the extracted nucleic acids with DNase, hybridizing mRNA of the total RNA contained in the whole single-cell with oligo (dT) fixed onto a carrier, performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and amplifying cDNA fixed onto the carrier.
    Type: Application
    Filed: August 1, 2014
    Publication date: June 11, 2015
    Inventors: Kiyomi TANIGUCHI, Hideki Kambara, Tomoharu Kajiyama
  • Patent number: 8975066
    Abstract: Accurate and sensitive sequencing in pyrosequencing is achieved by allowing complementary strand synthesis reaction to proceed homogeneously and completely in a short time while performing luminescence reaction for a sufficiently long time. DNA as a sequencing target is immobilized on the surface of a solid support. Nucleic acid substrates are injected from a dispenser to the support site where complementary strand synthesis is in turn performed rapidly and completely in a short time under a small reaction volume. Next, the support together with the product thereon is moved into a luminescence reaction solution where luminescence reaction is in turn performed. Thus, a DNA complementary strand synthesis reaction site and a luminescence reaction site are completely separated. The support surface is also washed by dipping the support in the luminescence reaction solution that contains a luminescence reagent and an enzyme that degrades redundant nucleic acid substrates.
    Type: Grant
    Filed: April 7, 2009
    Date of Patent: March 10, 2015
    Assignee: Hitachi, Ltd.
    Inventors: Hideki Kambara, Akihiko Kishimoto
  • Publication number: 20150050644
    Abstract: An object of the present invention is to provide a method capable of easily and directly performing DNA sequencing by directly detecting pyrophosphoric acid released by an enzymatic reaction, particularly with a DNA polymerase or a DNA ligase. The present invention provides a method and a device for detecting pyrophosphoric acid by measuring an electrical change occurring when a boronic acid group immobilized on an electrically conductive support reversibly binds to pyrophosphoric acid.
    Type: Application
    Filed: December 5, 2012
    Publication date: February 19, 2015
    Applicant: Hitachi, Ltd.
    Inventors: Hirokazu Nishida, Hideki Kambara
  • Publication number: 20150024959
    Abstract: The problem to be solved by the present invention is to provide a method for preparing a sample for comprehensively and accurately analyzing gene expression in a single cell or a few cells, for example, by a large-scale DNA sequencer.
    Type: Application
    Filed: November 21, 2012
    Publication date: January 22, 2015
    Inventors: Hiroyuki Tsunoda, Huan Huang, Mari Ohta, Hideki Kambara