Patents by Inventor Hideki Kambara
Hideki Kambara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12039508Abstract: Provided is an information processing system that, when a shopper purchases a product displayed in a shop, enables the automation of the purchase of the product and reduces the time required for purchasing the product. This information processing system comprises a moving object tracking means, a shelf product recognition means, and a purchase means. The moving object tracking means identifies moving objects, such as shoppers and baskets, that are moving through a shop, defines the region of the moving object, and picks up images while tracking the moving object. The shelf product recognition means constantly picks up images of the state of the shelves, compares images picked up before and after an object is taken from a shelf, defines the region from the picked up image of a recognized product, and identifies the product from the defined image region. The purchase means purchases the identified product.Type: GrantFiled: May 27, 2021Date of Patent: July 16, 2024Assignee: SIGNPOST CORPORATIONInventors: Yasushi Kambara, Hideki Kawabata, Toshiya Namikawa, Tomohiro Sasaki
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Patent number: 11680286Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: GrantFiled: May 18, 2021Date of Patent: June 20, 2023Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Patent number: 11214796Abstract: The present invention provides a method and/or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.Type: GrantFiled: June 13, 2017Date of Patent: January 4, 2022Assignee: HITACHI, LTD.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi
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Publication number: 20210388422Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: ApplicationFiled: May 18, 2021Publication date: December 16, 2021Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI, Maiko TANABE
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Patent number: 11053536Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: GrantFiled: July 30, 2012Date of Patent: July 6, 2021Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Publication number: 20180238775Abstract: The purpose of the present invention is to provide a sample collection system capable of repeatedly collecting a sample. The present invention discloses a system including: at least one or multiple hollow collection needle(s) each having a knife edge for collecting the sample from a biological specimen; and a syringe, a solenoid and a reservoir that serve as a liquid-inflow mechanism allowing a solution to flow into the collection needle, wherein the sample is ejected from the collection needle together with the solution so as to recover the sample.Type: ApplicationFiled: March 30, 2018Publication date: August 23, 2018Applicant: FRONTIER BIO SYSTEMS, INC.Inventors: Hideki KAMBARA, Masahito HOSOKAWA, Haruko TAKEYAMA, Takuya YODA
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Patent number: 10030240Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.Type: GrantFiled: March 12, 2013Date of Patent: July 24, 2018Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Patent number: 9862993Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a genetic analysis system, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.Type: GrantFiled: October 21, 2013Date of Patent: January 9, 2018Assignee: HITACHI, LTD.Inventors: Maiko Tanabe, Hideki Kambara, Masataka Shirai
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Publication number: 20170283796Abstract: The present invention provides a method and/or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.Type: ApplicationFiled: June 13, 2017Publication date: October 5, 2017Applicant: HITACHI, LTD.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi
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Patent number: 9708603Abstract: The problem to be solved by the present invention is to provide a method for preparing a sample for comprehensively and accurately analyzing gene expression in a single cell or a few cells, for example, by a large-scale DNA sequencer.Type: GrantFiled: November 21, 2012Date of Patent: July 18, 2017Assignee: HITACHI, LTD.Inventors: Hiroyuki Tsunoda, Huan Huang, Mari Ohta, Hideki Kambara
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Publication number: 20170016814Abstract: An object is to provide a biomolecule analyzer capable of collecting and analyzing a biomolecule in a single cell without damaging neighboring cells. In order to achieve this object, a biomolecule analyzer according to the present invention is characterized by including a unit which obtains an optical image of a plurality of cells, a unit which disrupts a part or the whole of at least one cell of the plurality of cells, an array device in which regions for capturing a biomolecule in the cell released by the disrupting unit are arranged, and a unit which associates the region in which the biomolecule is captured in the array device with a portion corresponding to the cell disrupted by the disrupting unit in the optical image.Type: ApplicationFiled: December 27, 2013Publication date: January 19, 2017Applicant: HITACHI, LTD.Inventors: Masataka Shirai, Hideki Kambara, Tomoharu Kajiyama
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Publication number: 20160333397Abstract: A reaction liquid after a nucleic acid amplification reaction is made suitable for various processes. A step of measuring the amount of a target product and the amount of a byproduct after performing a nucleic acid amplification reaction, and a step of determining that a process for removing the byproduct is needed when the abundance ratio of the target product to the byproduct is lower than a prescribed value, and determining the dilution ratio of a reaction liquid after the nucleic acid amplification reaction when the abundance ratio is higher than the prescribed value are included.Type: ApplicationFiled: January 27, 2014Publication date: November 17, 2016Applicant: HITACHI, LTD.Inventors: Hiroko Matsunaga, Tomoharu Kajiyama, Mari Ohta, Hideki Kambara
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Publication number: 20160251705Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a means, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.Type: ApplicationFiled: October 21, 2013Publication date: September 1, 2016Applicant: HITACHI, LTD.Inventors: Maiko Tanabe, Hideki Kambara, Masataka Shirai
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Patent number: 9428795Abstract: To provide a means and a method of quickly sampling a tissue fragment from a plant tissue, quickly preserving a gene expression state within the tissue fragment, and comprehensively analyzing the gene expression state. The present invention relates to a method of sampling a plant tissue section, comprising: inserting a first gel layer into a needle; arranging a plant tissue on a second gel layer; and passing the needle through the plant tissue together with the second gel layer, and sampling a section of the plant tissue in the needle.Type: GrantFiled: December 18, 2012Date of Patent: August 30, 2016Assignee: Hitachi, Ltd.Inventors: Tomoharu Kajiyama, Hideki Kambara, Toru Habu
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Patent number: 9278355Abstract: This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.Type: GrantFiled: November 13, 2008Date of Patent: March 8, 2016Assignee: Hitachi, Ltd.Inventors: Tomoharu Kajiyama, Masataka Shirai, Hideki Kambara
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Publication number: 20160010078Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.Type: ApplicationFiled: March 12, 2013Publication date: January 14, 2016Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI, Maiko TANABE
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Publication number: 20150302042Abstract: The present invention aims to provide a data analysis apparatus capable of clustering appropriately even when there is an exceptional datum resulted from an experimental error and the like. In the data analysis apparatus according to the invention, a cluster range parameter for stretching a cluster boundary is determined in advance according to the range of an experimental error which an experimental error datum describes. In the process of clustering, an exceptional datum which does not belong to any cluster is determined to belong to a cluster when an area at a distance determined by the cluster range parameter from the exceptional datum is contained in the cluster, and the exceptional datum is determined to form an independent cluster when even the area at the distance is not contained in any cluster (see FIG. 7).Type: ApplicationFiled: November 20, 2012Publication date: October 22, 2015Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI
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Publication number: 20150167063Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: ApplicationFiled: July 30, 2012Publication date: June 18, 2015Applicant: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Publication number: 20150159202Abstract: A method of synthesizing cDNA, in sequence, includes picking up a whole single-cell from a sample containing at least a single-cell having a cell membrane, lysing the cell membrane and extracting nucleic acids from the cell, degrading DNA of the extracted nucleic acids with DNase, hybridizing mRNA of the total RNA contained in the whole single-cell with oligo (dT) fixed onto a carrier, performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and amplifying cDNA fixed onto the carrier.Type: ApplicationFiled: August 1, 2014Publication date: June 11, 2015Inventors: Kiyomi TANIGUCHI, Hideki Kambara, Tomoharu Kajiyama
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Patent number: 8975066Abstract: Accurate and sensitive sequencing in pyrosequencing is achieved by allowing complementary strand synthesis reaction to proceed homogeneously and completely in a short time while performing luminescence reaction for a sufficiently long time. DNA as a sequencing target is immobilized on the surface of a solid support. Nucleic acid substrates are injected from a dispenser to the support site where complementary strand synthesis is in turn performed rapidly and completely in a short time under a small reaction volume. Next, the support together with the product thereon is moved into a luminescence reaction solution where luminescence reaction is in turn performed. Thus, a DNA complementary strand synthesis reaction site and a luminescence reaction site are completely separated. The support surface is also washed by dipping the support in the luminescence reaction solution that contains a luminescence reagent and an enzyme that degrades redundant nucleic acid substrates.Type: GrantFiled: April 7, 2009Date of Patent: March 10, 2015Assignee: Hitachi, Ltd.Inventors: Hideki Kambara, Akihiko Kishimoto