Abstract: A device for measuring an extracellular potential of a test cell includes a substrate having a well formed in a first surface thereof and a first trap hole formed therein. The well has a bottom. The first trap hole includes a first opening formed in the bottom of the well and extending toward a second face of the substrate, a first hollow section communicating with the first opening via a first connecting portion, and a second opening extending reaching the second surface and communicating with the first hollow section via a second connecting portion. The first connecting portion has a diameter smaller than a maximum diameter of the first hollow section, greater than a diameter of the second connecting portion, and smaller than a diameter of the test cell. The device can retain the test cell securely and accept chemicals and the test cell to be put into the device easily.

Abstract: A measurement device 10 includes a measurement cell 11. The measurement cell 11 includes a substrates 17 and a substrate 16 formed on the substrate 17. The substrates 16 and 17 are bonded to each other. Thus, a flow pass 9 is formed in the measurement cell 11. As shown in FIG. 3, on the substrate 17 of the measurement cell 11, provided are a concave well 19, a protruding microelectrode 1 connected to a terminal 4a and formed on a side wall surface of the well 19, communicating tubes 15a and 15b connected to both of side surfaces of the well 19 (i.e., the flow pass 9) to face to each other, resistance baths 6a and 6b connected to the communicating tubes 15a and 15b, thin film resistors 3a and 3b arranged in the resistance baths 6a and 6b, and a reference electrode 2 connected to a terminal 4b.

Abstract: A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided. A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

Abstract: A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

Abstract: A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

Abstract: A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

Abstract: A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of T or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of A, T or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of A, T or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of T or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: A device for detecting a physicochemical change in a biological sample comprising at least one measuring electrode provided by causing a conductive material to enter into a porous film, and a conductor connected to the measuring electrode.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be G or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is A, while a base adjacent with one base spaced apart is G. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be C; the third base from the 3? end to be any one of A, T or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of A, T or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be G or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is A, while a base adjacent with one base spaced apart is T. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be C; the third base from the 3? end to be any one of T, C or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is C, while a base adjacent with one base spaced apart is T. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T; the third base from the 3? end to be any one of T, G or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is C, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second and the third bases from the 3? end to be 5?-AT-3?, 5?-TA-3?, 5?-TT-3? or 5?-CT-3?; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.