Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, as well as a method for secretory production of a heterologous protein. A coryneform bacterium is described that has been modified to have a specific mutation so as to harbor a phoS gene, and when cultured, is able to to produce a heterologous protein by secretory production.

Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, as well as a method for secretory production of a heterologous protein. A coryneform bacterium is described that has been modified to have a specific mutation so as to harbor a phoS gene, and when cultured, is able to to produce a heterologous protein by secretory production.

Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.

Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.

Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.

Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.

Abstract: The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5?-end to 3?-end, and secretory producing the heterologous protein by coryneform bacteria.

Abstract: By culturing a methanol-assimilating bacterium which harbors a DNA construct which contains a promoter sequence that functions in the methanol-assimilating bacterium and a nucleotide sequence that encodes a polypeptide containing a signal sequence and an objective protein which is functionally connected to the promoter sequence, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.

Abstract: In order to attain excellent electrical conductivity and molding processability, a fuel cell separator to be produced by performing press molding on a preform in which expanded graphite is used as the main raw material is improved so that the preform is produced by a papermaking method, whereby the characteristics of the mechanical strength, the flexibility, and the gas impermeability are improved, and the moldability is made excellent so that a light and compact configuration that is preferred in the automobile use or the like can be realized.

Abstract: By culturing a methanol-assimilating bacterium which harbors a DNA construct which contains a promoter sequence that functions in the methanol-assimilating bacterium and a nucleotide sequence that encodes a polypeptide containing a signal sequence and an objective protein which is functionally connected to the promoter sequence, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.

Abstract: The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5?-end to 3?-end, and secretory producing the heterologous protein by coryneform bacteria.

Abstract: A method for manufacturing sponge iron and an apparatus for charging in the method are disclosed. Iron oxide powder and reducing-agent powder are charged such that alternating layers of the iron oxide powder and the reducing-agent powder are formed and such that each of the layers is in the form of a helix, and then a reduction treatment is performed. The method has not only high reaction efficiency of a gas, high quality, and high productivity, but also the advantage for a production adjustment because the amount of charge can be adjusted without the limitation of a reduction time. The molar ratio of the carbon content in the reducing agent to the oxygen content in the iron oxide in the reaction container is preferably 1.1 or more.

Abstract: An instruction code conversion unit, an information processing system provided with the instruction code conversion unit and an instruction code generation method for generating instruction codes which are converted by the instruction code conversion unit are described. The efficiency of coding of the program is improved by making use of an existing processor as selected is used without modification. An instruction code conversion unit performs conversion of the address of a native instruction code to the address of the corresponding compressed instruction code in a program memory by shifting the address of the native instruction code as outputted from the processor to the right by one bit.

Abstract: An instruction code conversion unit, an information processing system provided with the instruction code conversion unit and an instruction code generation method for generating instruction codes which are converted by the instruction code conversion unit are described. The efficiency of coding of the program is improved by making use of an existing processor as selected is used without modification. An instruction code conversion unit performs conversion of the address of a native instruction code to the address of the corresponding compressed instruction code in a program memory by shifting the address of the native instruction code as outputted from the processor to the right by one bit.

Abstract: A traveling hearth for producing reduced metal by charging and stacking a raw material containing a metal-containing material and a solid-reducing material on a horizontally moving hearth, arranged for disposing a solid-reducing material layer on the hearth, forming concave portions at the solid-reducing material surface, stacking the raw material on the surface of the solid-reducing material layer, reducing the raw material by at least once heating and melting the material on the hearth to separate metal and gangue and ash ingredients, and discharging metal from the hearth.

Abstract: Producing reduced metal by charging and stacking a raw material containing a metal-containing material and a solid reducing material on a horizontally moving hearth of a traveling hearth furnace, by disposing a solid reducing material layer on the hearth, forming concave portions at the solid reducing material surface, stacking the raw material on the surface of the solid reducing material layer, reducing raw material by at least once heating and melting the material on the hearth to separate metal and gangue and ash ingredients, and discharging metal from the hearth.

Abstract: Method and apparatus for collecting valuable products, particularly zinc, from a zinc-containing substance such as electric furnace dust. A shaft type smelting reduction furnace has a packed bed of a carbonaceous solid reducing agent and has upper and lower tuyeres, and a zinc-containing substance is injected through tuyeres to reduce and vaporize zinc, which is directly contacted by a liquid coolant thereby forming a slurry, and the zinc product is collected from the slurry.

Abstract: Disclosed herein is a gas dispersing device to be employed in a fluidized bed preliminary reducing furnace. The gas dispersing device is constructed of a plurality of rotatable round bars arranged in multiple stages so as to form a grate. The gas dispersing device includes a rotating mechanism for rotating each bar.According to the present invention, deformation of the bars due to a temperature change thereof can be avoided to thereby extend a life of the dispersing device. Furthermore, blinding of the dispersing device can be prevented to thereby effectuate a stable operation of a fluidized bed. Accordingly, productivity in the fluidized bed reducing furnace is improved, and labor saving is realized.

Abstract: A method and apparatus for producing molten metal from powder state ore through smelting process to be performed in a shaft furnace, utilized the reducing material having grain size greater than that n-times of the gas flow velocity corresponding grain size to charge from the top of a shaft furnace for forming fluidized bed at the upper section of the furnace and a reducing material filled section below the fluidized bed. The method and apparatus for smelting the powder state ore also takes the reducing material having smaller grain size to be blown into the furnace through tuyeres.

Abstract: A RAM the capacity of which is to be detected is constituted by 2.sup.N memory blocks each having a predetermined memory capacity. An address signal representing an existing memory capacity can be fed to the RAM. An address controller designates a predetermined address for each block of the RAM. A code write section sequentially writes a detection code at the predetermined address in each block of the RAM. A code readout section reads out the detection code from the predetermined address of the first block of the RAM designated by the address controller. A code comparator sequentially compares the detection code read out by the code readout section with a detection code last written by the code write section, so as to detect whether there is a coincidence therebetween, and, when a coincidence is obtained, the comparator is able to detect the capacity of the entire RAM based on the coincident detection code.