Abstract: The object of the present invention is to utilize, as a sensor protein, a molecular recognizing ability of a protein that scarcely undergoes any structural change by the binding of a target substance. According to the present invention, there is provided a sensor protein comprising an insert-type fusion protein composed of a reporter protein and a biding protein wherein said binding protein is inserted into the amino acid sequence of said reporter protein.

Abstract: The object of the present invention is to utilize, as a sensor protein, a molecular recognizing ability of a protein that scarcely undergoes any structural change by the binding of a target substance. According to the present invention, there is provided a sensor protein comprising an insert-type fusion protein composed of a reporter protein and a biding protein wherein said binding protein is inserted into the amino acid sequence of said reporter protein.

Abstract: A translation template comprising an ORF region coding for a protein, a 5? untranslated region comprising a transcription promoter and a translation enhancer and locating on the 5? side of the ORF region, and a 3? end region comprising a poly-A sequence and locating on the 3? side of the ORF region, is expressed in a translation system in the presence of an agent for modifying a C-terminal of a protein, which comprises an acceptor portion having a group capable of binding to a protein through a transpeptidation reaction in a protein translation system and a modifying portion comprising a nonradioactive modifying substance linked to the acceptor portion via a nucleotide linker, to cause protein synthesis and the synthesized protein is purified. Thus, the yield of modified protein in a method of modifying C-terminal of protein is improved and detection of protein interaction based on various intermolecular interaction detection methods is realized at an improved level.

Abstract: The present invention provides a method of constructing a DNA library comprising: (a) ligating building block DNAs in any combination; (b) selecting and mixing the obtained ligated building block DNAs such that all ligated building block DNAs to be used have an end sequence that overlaps with an end sequence of at least one other type of ligated building block DNA; and (c) performing Polymerase Chain Reaction using the mixture of ligated building block DNAs as a template to obtain a DNA library comprising 2 or more clones. The present invention provides a method of constructing a group of genes formed by ligation of a plurality of DNA fragments encoding any amino acid sequence in various orders and lengths.

Abstract: An assigning molecule obtained by ligating a genotype molecule to a phenotype molecule which is a protein encoded by the ORF region in the genotype molecule, by transpeptidation, the genotype molecule being constructed by bonding (a) a spacer molecule comprising a donor region which can be bonded to a 3′-terminal end of nucleic acid, a PEG region that is bonded to the donor region and comprises polyethylene glycol as a main component and a peptide acceptor region which is bonded to the PEG region and comprises a group which can be bonded to a peptide by transpeptidation, to (b) a 3′-terminal end of a coding molecule which is a nucleic acid comprising a 5′ untranslated region comrising a transcription promoter and a translation enhancer; an ORF region which is bonded to the 3′-terminal side of the 5′ untranslated region and encodes a protein; and a 3′-terminal region that is bonded to the 3′-terminal side of the ORF region and comprises a polyA sequence.

Abstract: A molecule assigning a genotype to a phenotype, which comprises a nucleic acid portion having a nucleotide sequence reflecting the genotype, and a protein portion comprising a protein involved in exhibition of the phenotype, a 3′-terminal end of the nucleic acid portion and a C-terminal end of the protein portion being covalently bound, and a method for constructing the molecule for assigning the genotype to the phenotype, which comprises (a) preparing a DNA containing a gene which has no termination codon, (b) transcribing the prepared DNA into RNA, (c) bonding a chimeric spacer composed of DNA and RNA to a 3′-terminal end of the obtained RNA, (d) bonding, to a 3′-terminal end of the obtained bonded product, a nucleoside or a substance having a chemical structure analogous to that of a nucleoside, which can be covalently bound to an amino acid or a substance having a chemical structure analogous to that of an amino acid, and (e) performing protein synthesis in a cell-free protein synthesis

Abstract: A molecule assigning a genotype to a phenotype, which comprises a nucleic acid portion having a nucleotide sequence reflecting the genotype, and a protein portion comprising a protein involved in exhibition of the phenotype, a 3′-terminal end of the nucleic acid portion and a C-terminal end of the protein portion being covalently bound, and a method for constructing the molecule for assigning the genotype to the phenotype, which comprises (a) preparing a DNA containing a gene which has no termination codon, (b) transcribing the prepared DNA into RNA, (c) bonding a chimeric spacer composed of DNA and RNA to a 3′-terminal end of the obtained RNA, (d) bonding, to a 3′-terminal end of the obtained bonded product, a nucleoside or a substance having a chemical structure analogous to that of a nucleoside, which can be covalently bound to an amino acid or a substance having a chemical structure analogous to that of an amino acid, and (e) performing protein synthesis in a cell-free protein synthesis

Abstract: A molecule comprising a nucleic acid portion and a protein portion directly bound to said nucleic acid portion with a covalent bond, wherein said nucleic acid portion comprises a polymer of nucleoside, and said protein portion is encoded by said nucleic acid portion, and a method for constructing the molecule, which comprises (a) preparing a DNA containing a gene which has no termination codon, (b) transcribing the prepared DNA into RNA, (c) bonding a chimeric spacer composed of DNA and RNA to a 3′-terminal end of the obtained RNA, (d) bonding to a 3′-terminal end of the obtained bonded product, a nucleoside or a substance having a chemical structure analogous to that of a nucleoside, which can be covalently bound to an amino acid or substance having a chemical structure analogous to that of an amino acid, and (e) performing protein synthesis in a cell-free protein synthesis system using the obtained bonded product as mRNA to bond a nucleic acid portion containing the gene to a translation product

Abstract: A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided.

Abstract: A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided.

Abstract: A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided.

Abstract: A method for manufacturing a cam shaft including the steps of preparing cam pieces provided with shaft holes and a tubular shaft member having a hollow portion, forming a plurality of grooves extending in the axial direction of the shaft hole at predetermined positions on the inner circumferential surfaces of the shaft holes of the cam pieces, fitting the shaft member onto the shaft holes of the cam pieces after arranging the cam pieces at the predetermined portions of a support tool and thereafter inserting under pressure a tube expanding tool provided with bulged portions corresponding to the grooves into the hollow portion of the shaft member to bulge and deform the shaft member in the radial direction so that the grooves and bulged portions are confronted with each other, thus to bond the cam pieces and the shaft member together.

Abstract: The invention resides in an improved time error measuring device or test bench for watches. The device includes a sensing microphone for sensing the stepping movement of the watch under test and in the form of a series of electric signal pulses. It further includes a range selector adapted for selecting a proper range depending upon the thus sensed stepping signal pulse series. Time error measuring means is utilized for detecting occasional time error of the watch by comparing the sensed stepping pulses with a standard clock pulse series and at the precision attributed to a selected measuring range. All these operations may be carried out in a fully automatic manner.