Abstract: A method prepares a sugar solution from cellulose-containing biomass. The method includes step (1): a step of obtaining an endoxylanase hydrolysate by hydrolyzing the cellulose-containing biomass using endoxylanase derived from microorganisms of the Acremonium genus or the Aspergillus genus, step (2): a step of separating the endoxylanase hydrolysate into an endoxylanase hydrolysate solid and an endoxylanase hydrolysate liquid through solid-liquid separation, step (3): a step of obtaining a cellulase hydrolysate by hydrolyzing the endoxylanase hydrolysate solid using cellulase derived from a filamentous fungus, and step (4): a step of filtering the cellulase hydrolysate through an ultrafiltration membrane to recover a sugar solution from the filtrate side and collect an enzyme component from the non-filtrate side.

Abstract: A method of producing a sugar liquid from a cellulose-containing biomass includes (1) hydrolyzing a cellulose-containing biomass using a filamentous fungus-derived cellulose and carrying out solid-liquid separation into a sugar liquid and a hydrolysis residue; (2) washing the hydrolysis residue in (1) using an aqueous alkaline solution and an aqueous inorganic salt solution independently and recovering, as a washing liquid, the filamentous fungus-derived cellulase adsorbed to the hydrolysis residue; and (3) filtering the washing liquid in (2) through an ultrafiltration membrane to recover a sugar liquid as a permeate and the filamentous fungus-derived cellulase as a non-permeate.

Abstract: A method of producing a sugar liquid derived from a cellulose-containing biomass includes (a) saccharifying a pretreated product having alignin content of not more than 8.5% obtained by pretreatment of a cellulose-containing biomass, to obtain a saccharified liquid; (b) filtering the saccharified liquid obtained in Step (a) through a microfiltration membrane to allow formation of a cake on a membrane surface in a feed side while obtaining a sugar liquid from a permeate side; and (c) collecting the cake formed on the membrane surface in Step (b) by peeling from the membrane.

Abstract: A method of producing a sugar liquid includes filtering a cellulose-derived sugar liquid through one or more separation membranes selected from the group consisting of an ultrafiltration membrane, a nanofiltration membrane and a reverse osmosis membrane, and washing the separation membrane(s) after filtration with washing water containing an acidic substance and an aromatic compound.

Abstract: A method of producing a sugar liquid and a xylooligosaccharide includes Steps (1) to (3): Step (1): hydrolyzing a cellulose-containing biomass with a filamentous fungus-derived cellulase; Step (2): subjecting the hydrolysate of Step (1) to solid-liquid separation, and filtering the solution component through an ultrafiltration membrane to recover cellulase as a non-permeate, and to recover a sugar liquid as a permeate; and Step (3): reacting the recovered cellulase in Step (2) with a xylan-containing material, and recovering a xylooligosaccharide produced, Step (3) being independent from Step (1).

Abstract: A method prepares a sugar solution from cellulose-containing biomass. The method includes step (1): a step of obtaining an endoxylanase hydrolysate by hydrolyzing the cellulose-containing biomass using endoxylanase derived from microorganisms of the Acremonium genus or the Aspergillus genus, step (2): a step of separating the endoxylanase hydrolysate into an endoxylanase hydrolysate solid and an endoxylanase hydrolysate liquid through solid-liquid separation, step (3): a step of obtaining a cellulase hydrolysate by hydrolyzing the endoxylanase hydrolysate solid using cellulase derived from a filamentous fungus, and step (4): a step of filtering the cellulase hydrolysate through an ultrafiltration membrane to recover a sugar solution from the filtrate side and collect an enzyme component from the non-filtrate side.

Abstract: A method of producing a sugar liquid includes: (A) reacting mannanase with a liquid component obtained by hydrolysis treatment of woody biomass to obtain a saccharified liquid; and (B) filtering the saccharified liquid in Step (A) through a microfiltration membrane and/or ultrafiltration membrane to collect a sugar liquid from a permeate side.

Abstract: A method produces a sugar liquid from a cellulosic biomass material, which sugar liquid has improved fermentability. The method produces a sugar liquid by: adding an alkali(s) to a concentrated cellulosic biomass sugar liquid to adjust the pH to not less than 7 to precipitate an insoluble substance(s) containing at least magnesium; and performing filtration through a microfiltration membrane to remove the insoluble substance(s), to obtain a sugar liquid as a permeate.

Abstract: An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.

Abstract: An apparatus that produces a sugar liquid by a method including hydrolyzing cellulose includes a hydrolysis tank to which a recovered enzyme feed pipe and a fresh enzyme feed pipe are connected; a device for solid-liquid separation of a hydrolysate; a sugar liquid-retaining tank having a water supply pipe that washes an ultrafiltration membrane and/or removes recovered enzyme retained in a circulation pipe; and an ultrafiltration membrane device that separates enzyme and a sugar liquid.

Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).

Abstract: An apparatus for treating a biomass feedstock at a high temperature, includes a cooling device 90 for cooling a biomass treated liquid at a high temperature; an enzymatic saccharification tank 103 for saccharifying a cooled treated liquid 101B with an enzyme; a solid-liquid separation apparatus 112 for removing water-slightly soluble substances contained in a saccharide solution 104 taken out from the enzymatic saccharification tank 103 and a foreign substance removing unit 113 provided with a microfiltration (MF) membrane 113a; a dilution tank 132, disposed downstream of the foreign substance removing unit 113, for diluting the saccharide solution from which the water-slightly soluble substances are removed by adding water thereto; a water separation unit 116, provided with a reverse osmosis (RO) membrane 116a, for removing water 114 from the diluted saccharide solution so as to obtain concentrated saccharide solution 115.

Abstract: A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.

Abstract: A method of producing a sugar solution includes (1) to (3): (1) preparing a slurry of a cellulose-containing biomass pretreated material and an inactivated cellulose, (2) hydrolyzing the slurry in (1) by adding a filamentous fungus-derived cellulase to the slurry, and (3) separating a hydrolysate in (2) into a solution component and a hydrolysis residue through solid-liquid separation, and filtering the solution component through an ultrafiltration membrane, thereby recovering the filamentous fungus-derived cellulase as a non-filtrate and the sugar solution as a filtrate.

Abstract: A method of producing a sugar liquid, includes filtering a cellulose-derived sugar liquid through one or more separation membranes selected from the group consisting of ultrafiltration membranes, nanofiltration membranes and reverse osmosis membranes, and washing the separation membrane(s) after filtration with warm water at a temperature of not less than 50° C.

Abstract: An apparatus for treating a biomass feedstock at a high temperature, includes a cooling device 90 for cooling a biomass treated liquid at a high temperature; an enzymatic saccharification tank 103 for saccharifying a cooled treated liquid 101B with an enzyme; a solid-liquid separation apparatus 112 for removing water-slightly soluble substances contained in a saccharide solution 104 taken out from the enzymatic saccharification tank 103 and a foreign substance removing unit 113 provided with a microfiltration (MF) membrane 113a; a dilution tank 132, disposed downstream of the foreign substance removing unit 113, for diluting the saccharide solution from which the water-slightly soluble substances are removed by adding water thereto; a water separation unit 116, provided with a reverse osmosis (RO) membrane 116a, for removing water 114 from the diluted saccharide solution so as to obtain concentrated saccharide solution 115.

Abstract: A method of manufacturing coumaramide includes treating cellulose-based biomass with an ammonia-containing treatment agent to obtain ammonia-treated biomass, immersing the ammonia-treated biomass into a solvent containing at least water and eluting coumaramide in the ammonia-treated biomass into the solvent to obtain a coumaramide solution, and precipitating coumaramide in the coumaramide solution as a crystal to obtain a coumaramide crystal.

Abstract: A sugar solution production apparatus includes a saccharification tank that obtains a saccharified solution containing a solid substance from a cellulose-containing biomass, a solid-liquid separator that separates the solid substance from the saccharified solution to obtain a sugar solution, an enzyme recovery solution tank that stores an enzyme recovery solution, a sugar solution extraction line that extracts the sugar solution from the solid-liquid separator, a warm water supply unit that supplies warm water to the solid-liquid separator, an enzyme recovery solution supply line that supplies the enzyme recovery solution from the enzyme recovery solution tank to the solid-liquid separator, an enzyme recovery solution recovery line that feeds the enzyme recovery solution containing the saccharifying enzyme recovered from the solid substance to the enzyme recovery solution tank, and an enzyme recovery solution return line that returns the enzyme recovery solution to the saccharification tank.

Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes step (1) of hydrolyzing cellulose-containing biomass by a filamentous fungus-derived cellulase; and step (2) of filtering a hydrolysate obtained in step (1) through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate, wherein the cellulose-containing biomass is treated with one or more enzymes selected from the group consisting of pectinase, glucoamylase, and lipase at a stage previous to step (1) or in step (1).

Abstract: A method of producing a sugar liquid includes steps (1) providing a pretreatment product of a first cellulose-containing biomass; (2) adding a second cellulose-containing biomass or a pretreatment product thereof in an amount ranging from 1 to 50% based on the solid content weight of the first cellulose-containing biomass pretreatment product of step (1), and carrying out hydrolysis by a filamentous fungus-derived cellulase; and (3) subjecting a hydrolysate of step (2) to solid-liquid separation to obtain a solution component and a hydrolysis residue, and filtering the solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a retentate and recover a sugar liquid as a permeate.