Patents by Inventor Hisahiro Tabuchi

Hisahiro Tabuchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210246193
    Abstract: Provided is a method capable of producing a protein at a high level using a cultured animal cell, comprising culturing a cell that expresses APES (Antibody Production Enhancing Sequence) and into which a DNA encoding a desired polypeptide has been introduced, thereby producing the desired polypeptide. APES contains a nucleotide sequence related to nuclear factor ?B inhibitor ? (NfkBia) and has a function of decreasing the intracellular expression of NfkBia.
    Type: Application
    Filed: April 23, 2021
    Publication date: August 12, 2021
    Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventors: Hisahiro TABUCHI, Tomoya SUGIYAMA
  • Patent number: 11028149
    Abstract: Provided is a method capable of producing a protein at a high level using a cultured animal cell, comprising culturing a cell that expresses APES (Antibody Production Enhancing Sequence) and into which a DNA encoding a desired polypeptide has been introduced, thereby producing the desired polypeptide. APES contains a nucleotide sequence related to nuclear factor ?B inhibitor ? (NfkBia) and has a function of decreasing the intracellular expression of NfkBia.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: June 8, 2021
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 9802993
    Abstract: The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly taurine transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.
    Type: Grant
    Filed: October 14, 2008
    Date of Patent: October 31, 2017
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 9499618
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
    Type: Grant
    Filed: July 16, 2015
    Date of Patent: November 22, 2016
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 9284374
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: March 15, 2016
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 9238687
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein at low cost. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses a taurine transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster taurine transporter, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: January 19, 2016
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama, Saeko Tanaka, Satoshi Tainaka
  • Patent number: 9181540
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: November 10, 2015
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
  • Publication number: 20150315624
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
    Type: Application
    Filed: July 16, 2015
    Publication date: November 5, 2015
    Applicant: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Publication number: 20150275230
    Abstract: An object of the present invention is to establish a cell line that is useful as a host cell for use in recombinant protein production, highly expresses transgenes stably, and grows stably. The present invention provides a method for establishing a cell line for recombinant protein production capable of stably expressing two or more foreign genes, comprising transferring a gene of a non-coding RNA suppressing the expression of NfkBia to a cell.
    Type: Application
    Filed: October 10, 2013
    Publication date: October 1, 2015
    Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventor: Hisahiro Tabuchi
  • Patent number: 9068212
    Abstract: The present invention provides a method capable of producing a protein efficiently. A method of producing a polypeptide, comprising culturing a cell which strongly expresses a bicarbonate transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce said polypeptide.
    Type: Grant
    Filed: October 23, 2008
    Date of Patent: June 30, 2015
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
  • Publication number: 20140335565
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.
    Type: Application
    Filed: August 1, 2014
    Publication date: November 13, 2014
    Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
  • Publication number: 20140309405
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
    Type: Application
    Filed: March 26, 2014
    Publication date: October 16, 2014
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 8796007
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.
    Type: Grant
    Filed: February 8, 2012
    Date of Patent: August 5, 2014
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
  • Patent number: 8741601
    Abstract: The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly bicarbonate transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.
    Type: Grant
    Filed: April 21, 2010
    Date of Patent: June 3, 2014
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 8697397
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
    Type: Grant
    Filed: August 6, 2008
    Date of Patent: April 15, 2014
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Publication number: 20140030758
    Abstract: Provided is a method capable of producing a protein at a high level using a cultured animal cell, comprising culturing a cell that expresses APES (Antibody Production Enhancing Sequence) and into which a DNA encoding a desired polypeptide has been introduced, thereby producing the desired polypeptide. APES contains a nucleotide sequence related to NfkBia and has a function of decreasing the intracellular expression of NfkBia.
    Type: Application
    Filed: March 30, 2012
    Publication date: January 30, 2014
    Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Publication number: 20120142057
    Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.
    Type: Application
    Filed: February 8, 2012
    Publication date: June 7, 2012
    Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
  • Publication number: 20120045795
    Abstract: The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly bicarbonate transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.
    Type: Application
    Filed: April 21, 2010
    Publication date: February 23, 2012
    Inventors: Hisahiro Tabuchi, Tomoya Sugiyama
  • Patent number: 8062863
    Abstract: A polypeptide selected from the group consisting of: (1) a polypeptide having the amino acid sequence of SEQ ID NO: 1, and (2) a polypeptide having an amino acid sequence in which the third amino acid of the amino acid sequence of SEQ ID NO: 1 is substituted by another amino acid and having insulin-like growth factor 1 (IGF-1) activity; a DNA encoding said polypeptide; a vector containing said DNA; a host cell containing said vector; and a method for preparing a recombinant protein using said polypeptide.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: November 22, 2011
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hisahiro Tabuchi, Saeko Tanaka
  • Publication number: 20110014654
    Abstract: The present invention provides a method capable of producing a protein efficiently. A method of producing a polypeptide, comprising culturing a cell which strongly expresses a bicarbonate transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce said polypeptide.
    Type: Application
    Filed: October 23, 2008
    Publication date: January 20, 2011
    Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama