Abstract: It is intended to provide a kit or a device for the detection of esophageal cancer and a method for detecting esophageal cancer. The present invention provides a kit or a device for the detection of esophageal cancer, comprising nucleic acid(s) capable of specifically binding to miRNA(s) in a sample f a subject, and a method for detecting esophageal cancer, comprising measuring the miRNA in vitro.

Abstract: It is intended to provide a kit or a device for the detection of breast cancer and a method for detecting breast cancer. The present invention provides a kit or a device for the detection of breast cancer, comprising nucleic acid(s) capable of specifically binding to a miRNA in a sample of a subject, and a method for detecting breast cancer, comprising measuring the miRNA in vitro.

Abstract: This invention relates to a kit or a device for the detection of stomach cancer and a method for detecting stomach cancer, and provides a kit or a device for the detection of stomach cancer, comprising a nucleic acid(s) capable of specifically binding to a miRNA(s) in a sample from a subject, and a method for detecting stomach cancer, comprising measuring the miRNA(s) in vitro.

Abstract: An object of the present invention is to provide a kit or a device for the detection of prostate cancer and a method for detecting prostate cancer. The present invention provides a kit or a device for the detection of prostate cancer, comprising a nucleic acid capable of specifically binding to a miRNA in a sample of a subject, and a method for detecting prostate cancer, comprising measuring the miRNA in vitro.

Abstract: The present invention provides a kit or device for the detection of biliary tract cancer, and a method for detecting biliary tract cancer. The present invention relates to a kit or device for the detection of biliary tract cancer, comprising a nucleic acid capable of specifically binding to miRNA in a sample of a subject, and a method for detecting biliary tract cancer, comprising measuring the miRNA in vitro.

Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.

Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.

Abstract: A method of analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, said method comprising a determining step of whether said RNA satisfies: B/A?1 wherein A represents the weight ratio (%) of RNA within the range of 1000 to 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis; and B represents the weight ratio (%) of RNA within the range of more than 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis.

Abstract: A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip includes a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method including injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and rotating said analysis chip to which said test substance-containing solution is injected applying a centrifugal acceleration of not less than 1×g.

Abstract: This invention relates to a composition, kit, or DNA chip for use in diagnosis of esophageal cancer, which comprises a plurality of polynucleotides selected from the group consisting of polynucleotides whose expression levels are varied in esophageal cancer tissues obtained from esophageal cancer patients when compared with cancer-free esophageal tissues obtained from esophageal cancer patients, mutants thereof, and fragments thereof, and to a method for detecting esophageal cancer using the composition, kit, or DNA chip.

Abstract: A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises: (a) phenol in an amount of more than 50% by volume based on the total amount of the solution; (b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution; (c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution; (d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and (e) a buffer for maintaining the pH of the solution at 4 to 6.

Abstract: The method of stirring a solution according to the present invention is a method of stirring a solution comprising contacting a selective binding substance immobilized on the surface of a carrier with a solution containing an analyte substance reactive with the selective binding substance, and mixing the fine particles or air bubbles into the solution containing an analyte substance, and moving the fine particles or air bubbles without allowing contact thereof with the selective binding substance-immobilized surface. The present invention provides a stirring method that accelerates the reaction of a carrier-immobilized selective binding substance with an analyte substance and detects a trace amount of analyte at high signal intensity and high S/N ratio. The present invention enables diagnosis and examination in the clinical setting by using a selective binding substance-immobilized carrier such as DNA chip.

Abstract: A method of analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, said method comprising a determining step of whether said RNA satisfies: B/A?1 wherein A represents the weight ratio (%) of RNA within the range of 1000 to 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis; and B represents the weight ratio (%) of RNA within the range of more than 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis.

Abstract: A method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative includes an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5?-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol % and less than 25 mol % with respect to the total of uridine 5?-triphosphate (UTP) and AA-UTP.

Abstract: This invention relates to a composition, kit, or DNA chip comprising polynucleotides and antibodies as probes for detecting, determining, or predicting the presence or metastasis of esophageal cancer, and to a method for detecting, determining, or predicting the presence or metastasis of esophageal cancer using the same.

Abstract: This invention relates to a composition, kit, or DNA chip comprising polynucleotides and antibodies as probes for detecting, determining, or predicting the presence or metastasis of esophageal cancer, and to a method for detecting, determining, or predicting the presence or metastasis of esophageal cancer using the same.

Abstract: This invention relates to a composition, kit, or DNA chip for use in diagnosis of esophageal cancer, which comprises a plurality of polynucleotides selected from the group consisting of polynucleotides whose expression levels are varied in esophageal cancer tissues obtained from esophageal cancer patients when compared with cancer-free esophageal tissues obtained from esophageal cancer patients, mutants thereof, and fragments thereof, and to a method for detecting esophageal cancer using the composition, kit, or DNA chip.

Abstract: This invention relates to a composition, kit, or DNA chip comprising polynucleotides and antibodies as probes for detecting, determining, or predicting the presence or metastasis of esophageal cancer, and to a method for detecting, determining, or predicting the presence or metastasis of esophageal cancer using the same.

Abstract: This invention relates to a method for detecting in vitro a urothelial cancer, comprising measuring CXCL1 protein, or expression of a gene encoding the protein, in a biological sample from a subject, and to a kit for diagnosing a urothelial cancer comprising an antibody or nucleic acid probe, which is capable of binding specifically to the CXCL1 protein or a gene encoding the protein, respectively.

Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.