Abstract: The present invention pertains to methods for characterizing proteins in a sample using native capillary electrophoresis-mass spectrometry. The present invention pertains to methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample, detecting and/or discriminating between antibodies in an antibody mixture, and characterizing monospecific antibody side products in a bispecific antibody sample.

Abstract: Methods for identifying viral protein constituents and quantifying the relative abundance of such viral protein constituents in a sample of viral particles are disclosed. In embodiments, the methods include first-dimension chromatography to separate intact viral capsid components of the sample, online denaturation of the viral capsid components to produce intact viral proteins, second-dimension chromatography to separate the viral proteins, and mass spectrometry to determine the masses of the viral proteins and identify the viral protein constituents of the sample.

Abstract: Disclosed is a lycopene food colorant having a fresh-keeping function. The food colorant is prepared from the following raw materials in parts by weight: 1-3 parts of a lycopene crystal, 5-20 parts of an emulsifier, 0.5-5 parts of an antioxidant, 60-70 parts of glycerol, and 10-20 parts of water. The food colorant is prepared by mixing the raw materials and then emulsifying and grinding same. The granularity of the obtained lycopene colorant is moderate; the tone is suitable for coloring a minced fillet product; the lycopene colorant has high tinting strength, achieves a bright color, and has the effect of triggering myoglobin, so that the minced fillet product has a bright color and appears fresher; the lycopene colorant further has a fresh-keeping function and can kill some food microorganisms, so as to keep food fresh and prolong the shelf life.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: Disclosed is a lycopene food colorant having a fresh-keeping function. The food colorant is prepared from the following raw materials in parts by weight: 1-3 parts of a lycopene crystal, 5-20 parts of an emulsifier, 0.5-5 parts of an antioxidant, 60-70 parts of glycerol, and 10-20 parts of water. The food colorant is prepared by mixing the raw materials and then emulsifying and grinding same. The granularity of the obtained lycopene colorant is moderate; the tone is suitable for coloring a minced fillet product; the lycopene colorant has high tinting strength, achieves a bright color, and has the effect of triggering myoglobin, so that the minced fillet product has a bright color and appears fresher; the lycopene colorant further has a fresh-keeping function and can kill some food microorganisms, so as to keep food fresh and prolong the shelf life.

Abstract: The present invention provides a substrate having a unidirectional water transport property, the substrate comprised of a fluid permeable structure and including: an inner side surface; and an outer side surface having a higher absorbent capacity than the inner side surface, wherein the inner side surface has a hydrophobic surface layer extending continuously over at least one section thereof, the hydrophobic surface layer having a predetermined thickness which, in use, produces a substantial hydrophobic property to contacting water, whilst allowing for water contacting the inner side surface of the substrate to wick through the hydrophobic surface layer into the substrate; and wherein the substrate is respectively comprised of hydrophobic channels and hydrophilic channels which respectively extend between the inner side surface and the outer side surface.

Abstract: Methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample. The methods including: contacting a sample comprising one or more antibodies of interest with a protease to digest the sample into antibody fragments; separating antibody fragments by molecular weight and/or charge in one or more capillaries using capillary electrophoresis; eluting separated antibody fragments from the one or more capillaries; and determining the mass of the eluted antibody fragments by mass spec analysis, thereby detecting and/or discriminating between post-translational modification variants of the antibody of interest.

Abstract: The present invention provides methods to quickly and efficiently assess the effect of antibody variants, including PTMs, e.g., glycosylation, oxidation, and deamidation variants, on the binding between the antibody and FcRn. In particular, the present invention is based, at least in part, on the development of an online, two dimension liquid chromatography (2D-LC) method for assessing binding between antibody variants and FcRn. In one aspect, the online 2D-LC is coupled with mass spectrometry (MS). The present invention allows for differentiation of antibody variants by peak pattern and retention time profile by affinity chromatography, and identification of these variants by mass spectrometry analysis.

Abstract: The present invention provides a substrate having a unidirectional water transport property, the substrate comprised of a fluid permeable structure and including: an inner side surface; and an outer side surface having a higher absorbent capacity than the inner side surface, wherein the inner side surface has a hydrophobic surface layer extending continuously over at least one section thereof, the hydrophobic surface layer having a predetermined thickness which, in use, produces a substantial hydrophobic property to contacting water, whilst allowing for water contacting the inner side surface of the substrate to wick through the hydrophobic surface layer into the substrate; and wherein the substrate is respectively comprised of hydrophobic channels and hydrophilic channels which respectively extend between the inner side surface and the outer side surface.

Abstract: A method and apparatus for detecting a stale number. A stale number detection device obtains a call number, a call number contact creation date, and a call number issue date for the call number. The stale number detection device determines that a stale number state exists when the call number issue date is later than the call number contact creation date. The stale number detection device generates a stale number notification based on the stale number state.

Abstract: A method and apparatus for detecting a stale number. A stale number detection device obtains a call number, a call number contact creation date, and a call number issue date for the call number. The stale number detection device determines that a stale number state exists when the call number issue date is later than the call number contact creation date. The stale number detection device generates a stale number notification based on the stale number state.

Abstract: Disclosed herein is an energy efficient vertical cryogenic tank, which comprises a tank body with a vacuum insulation interlayer, wherein the tank body comprises a transmission means including an air return pipe and a liquid outlet pipe, and positioning means including supporting legs provided at the bottom of the tank body, and a built-in saturation adjustment mechanism formed by a heat exchanger connected to an let of the air return pipe and a return air dispersing device. By using a saturation adjustment mechanism at the inlet of the air return pipe, the cryogenic storage tank can not only fully leverage the gasification gas produced at the pump, but also achieve the saturation function of the LNG in the tank, with such benefits as reduced energy loss, simplified tank interface settings, improved efficiency of saturation adjustment, and avoided pump cavitation.

Abstract: Disclosed herein is a tank-pump integrated structure for an LNG filling station, which comprises a tank body having heat insulation layer space, a siphon package at the bottom of the tank body and a pump sump arranged in the siphon package. Moreover, a first heat insulation pipeline in the siphon package is connected to an LNG inlet on the pump sump, and a gas return port of the pump sump is coupled with the tank body by means of a second heat insulation pipeline. An on-off valve is arranged on the first heat insulation pipeline between the tank body and the pump sump, a shut-off valve is additionally arranged on the second heat insulation pipeline between the gas return inlet and the tank body, and heat insulation devices are provided external to the on-off valve and the shut-off valve respectively. The integrated structure can reduce investment costs, shorten the construction period, and facilitate maintenance of the submersible pump via the valves.

Abstract: Disclosed herein is an energy efficient vertical cryogenic tank, which comprises a tank body with a vacuum insulation interlayer, wherein the tank body comprises a transmission means including an air return pipe and a liquid outlet pipe, and positioning means including supporting legs provided at the bottom of the tank body, and a built-in saturation adjustment mechanism formed by a heat exchanger connected to an let of the air return pipe and a return air dispersing device. By using a saturation adjustment mechanism at the inlet of the air return pipe, the cryogenic storage tank can not only fully leverage the gasification gas produced at the pump, but also achieve the saturation function of the LNG in the tank, with such benefits as reduced energy loss, simplified tank interface settings, improved efficiency of saturation adjustment, and avoided pump cavitation.

Abstract: The present invention relates to the method and equipment for inspecting nuclear experimental locales, and particularly relates to the method and system for separating and measuring 37Ar quickly. The method of the invention comprises the steps of sampling, eliminating impurities, separating, purifying, measuring the sum of Ar, collecting Ar, measuring the activity of 37Ar, etc. The control unit in the system of the invention connects respectively to a sampling unit, a separating-purifying unit, and a radioactivity measuring unit which are connected in turn, and a computer and the software of the control unit are in charge of the automatic operation, measurement and data-collection of the whole system. The method and system for quickly separating and measuring 37Ar of the patent can meet the requirements of locales inspection for Comprehensive Test Ban Treaty (CTBT), the sensitivity of measuring 37Ar is high, so do the production and purity of Ar.

Abstract: The present invention relates to the method and equipment for inspecting nuclear experimental locales, and particularly relates to the method and system for separating and measuring 37Ar quickly. The method of the invention comprises the steps of sampling, eliminating impurities, separating, purifying, measuring the sum of Ar, collecting Ar, measuring the activity of 37Ar, etc. The control unit in the system of the invention connects respectively to a sampling unit, a separating-purifying unit, and a radioactivity measuring unit which are connected in turn, and a computer and the software of the control unit are in charge of the automatic operation, measurement and data-collection of the whole system. The method and system for quickly separating and measuring 37Ar of the patent can meet the requirements of locales inspection for Comprehensive Test Ban Treaty (CTBT), the sensitivity of measuring 37Ar is high, so do the production and purity of Ar.

Abstract: This invention relates to substituted 6-membered N-Herterocyclic neurotrophic compounds of formula (I) or pharmaceutically acceptable salts or hydrates thereof, wherein R1, R2, X, Y, and Z are as defined in the description; their preparation methods, compositions comprising the same, and their use as inhibitors of FK560 binding proteases activity for treating and preventing neurodegenerative diseases and other nerve disorders associated with nerve injuries or other related diseases