Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5?-end extension primer, wherein the single-stranded 5?-end extension primer comprises a sequencing platform adaptor sequence of a 5? end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3?-end adaptor sequence to the 3? end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.

Abstract: The invention provides pharmaceutical compositions and methods of use thereof for treating Prader-Willi syndrome. More specifically, the invention provides pharmaceutical compositions that when administered inhibit the G9a driven methylation of histone H3 lysine 9.

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3?-terminal and the 5?-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3?-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Abstract: Provided are techniques for barcode processing. A barcode is detected. Then the barcode is classified as any of a first class code that needs to be preserved and a second class code that does not need to be preserved. For the barcode classified as the first class code, a content referred to by the barcode is saved.

Abstract: A method for indexing with redundant information. The method may identify unknown code points for a document in response to an indexing request for the document. The method may further convert the identified unknown code points into a plurality of converted code points. Each set of converted code points of the plurality uses a different codepage. The method may further identify sets of same code points and sets of redundant code points from the plurality of converted code points. The method may build an index based on the sets of same code points and the sets of redundant code points.

Abstract: In one embodiment, in response to a route from a source location to a target location, the route is analyzed to identify a list of one or more driving scenarios along the route that match one or more predetermined driving scenarios. The route is segmented into a list of route segments based on the driving scenarios. At least one of the route segments corresponds to one of the identified driving scenarios. A path is generated based on the route segments for driving an autonomous driving vehicle from the source location to the target location. The path includes a number of path segments corresponding to the route segments. At least one of the path segments of the path is determined based on a preconfigured path segment of a predetermined driving scenario associated with the path segment, without having to calculating the same at real time.

Abstract: An equilibrium-conductance-compensated globally-linear symmetric method for obtaining power flows in a DC power network, including: establishing an equilibrium-conductance-compensated globally-linear function that relates all bus translation voltages to a bus injection power according to given bus load parameters and given bus source parameters of the DC power network; establishing an equilibrium-conductance-compensated globally-linear symmetric matrix-equation model for power flows in the DC power network according to the equilibrium-conductance-compensated globally-linear function and a given slack bus serial number; establishing an equilibrium-conductance-compensated globally-linear symmetric matrix relation between non-slack bus injection powers and all the bus translation voltages by using M-P inversion of matrices according to the equilibrium-conductance-compensated globally-linear symmetric matrix-equation model; and calculating each bus per-unit voltage and each branch-transferred power in the DC powe

Abstract: An equivalent-conductance-compensated eccentric method for obtaining power transfer coefficients of a direct current (DC) power network, including: establishing an equivalent-conductance-compensated globally-linear function that relates all bus translation voltages to a bus injection power according to given bus load parameters and given bus source parameters of the DC power network; establishing an equivalent-conductance-compensated globally-linear eccentric matrix-equation model for steady state of the DC power network; establishing an equivalent-conductance-compensated globally-linear eccentric matrix relation between non-reference bus injection powers and non-reference bus translation voltages by using ordinary inversion of matrices; establishing an equivalent-conductance-compensated globally-linear eccentric expression of a branch-transferred power in terms of the non-reference bus injection powers; and obtaining power transfer coefficients of the DC power network according to the equivalent-conductance-

Abstract: An asynchronous capacitance-to-digital converter (CDC) is described that allows for very low-power operation when during inactive periods (when no conductive object is in contact or proximity to the sensing electrodes). Asynchronous operation of the CDC provides for capacitance-to-digital conversion without the use of system resources and more power intensive circuit elements.

Abstract: A method for distributed file transfers with high performance and reliability includes creating, on a first Trivial File Transfer Protocol (TFTP) server, a global cache, where the global cache is used to store up to a first portion of a data file. The method further includes storing, on the first TFTP server, in the global cache, one or more continuous data blocks that have exceeded a defined first request rate threshold, where the one or more continuous data blocks make up a subset of the data blocks of the first portion of the data file. The method further includes predicting, on the first TFTP server, a next data block in the data file to be stored in the global cache and in response to predicting the next data block, storing, on the first TFTP server, the next data block in the global cache.

Abstract: A method for distributed file transfers with high performance and reliability includes creating, on a first Trivial File Transfer Protocol (TFTP) server, a global cache, where the global cache is used to store up to a first portion of a data file. The method further includes storing, on the first TFTP server, in the global cache, one or more continuous data blocks that have exceeded a defined first request rate threshold, where the one or more continuous data blocks make up a subset of the data blocks of the first portion of the data file. The method further includes predicting, on the first TFTP server, a next data block in the data file to be stored in the global cache and in response to predicting the next data block, storing, on the first TFTP server, the next data block in the global cache.

Abstract: A computing system performs file conversion upon format expiration. A computing system evaluates a risk of expiration of a first format associated with a first format object. The computing system determines if the risk of expiration is above a threshold. The computing system identifies in response to determining the risk of expiration is above the threshold, one or more files stored in the first format for conversion. The computing system converts one or more files stored in the first format to a second format. The computing system saves the one or more files in the second format.

Abstract: A method for indexing with redundant information. The method may identify unknown code points for a document in response to an indexing request for the document. The method may further convert the identified unknown code points into a plurality of converted code points. Each set of converted code points of the plurality uses a different codepage. The method may further identify sets of same code points and sets of redundant code points from the plurality of converted code points. The method may build an index based on the sets of same code points and the sets of redundant code points.

Abstract: A system and method to screen for malignant gliomas, other brain tumors, and brain injuries use disturbance coefficient, differential impedances, and artificial neural networks. The system uses prescribed excitation signals with several system configurations to measure the differential impedances, calculate harmonic responses and nonlinearity of brain tissue, and estimate the disturbance coefficient that indicates the likelihood of malignant gliomas, other brain tumors, and brain injuries. The disturbance coefficient is a weighted sum of many parameters such as receiving differential impedances, transmission differential impedances, harmonic responses, frequency dispersion, and nonlinear responses using different system configurations and different excitation signals. The method includes arranging the transmitters, receivers, and transmission lines to maximize the sensitivity of detecting brain tissue condition.

Abstract: Disclosed are a method of detecting a pre-determined event in a nucleic acid sample and a system thereof. The method of detecting the pre-determined event in the nucleic acid sample comprises the following steps: constructing a sequencing-library for the nucleic acid sample; sequencing the sequencing-library to obtain a sequencing result consisting of a plurality of sequencing data; determining the sequencing data from a pre-determined region; and determining an occurrence of the pre-determined event in the nucleic acid sample based on a composition of the sequencing data from the pre-determined region.

Abstract: An equivalent-conductance-compensated globally-linear symmetric method for obtaining power flows in a DC power network, including: establishing an equivalent-conductance-compensated globally-linear function that relates all bus translation voltages to a bus injection power according to given bus load parameters and given bus source parameters of the DC power network; establishing an equivalent-conductance-compensated globally-linear symmetric matrix-equation model for power flows in the DC power network according to the equivalent-conductance-compensated globally-linear function and a given slack bus serial number; establishing an equivalent-conductance-compensated globally-linear symmetric matrix relation between non-slack bus injection powers and all the bus translation voltages by using M-P inversion of matrices according to the equivalent-conductance-compensated globally-linear symmetric matrix-equation model; and calculating each bus per-unit voltage and each branch-transferred power in the DC power netw

Abstract: An equilibrium-conductance-compensated eccentric method for obtaining power transfer coefficients of a direct current (DC) power network, including: establishing an equilibrium-conductance-compensated globally-linear function that relates all bus translation voltages to a bus injection power according to given bus load parameters and given bus source parameters of the DC power network; establishing an equilibrium-conductance-compensated globally-linear eccentric matrix-equation model for steady state of the DC power network; establishing an equilibrium-conductance-compensated globally-linear eccentric matrix relation between non-reference bus injection powers and non-reference bus translation voltages by using ordinary inversion of matrices; establishing an equilibrium-conductance-compensated globally-linear eccentric expression of a branch-transferred power in terms of the non-reference bus injection powers; and obtaining power transfer coefficients of the DC power network according to the equilibrium-conduc

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.