Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

Abstract: The present disclosure provides a method and systems for processing or analyzing a nucleic acid molecule. A method for processing or analyzing a double-stranded nucleic molecule may comprise providing the double-stranded nucleic acid molecule and a double-stranded adapter. The double-stranded adapter may comprise a nicking site within a sense strand or an anti-sense strand of the double-stranded adapter. The double-stranded adapter may then be coupled to the double-stranded nucleic acid molecule, and the double-stranded nucleic acid molecule coupled to the double-stranded adapter may be circularized to generate a circularized double-stranded nucleic acid molecule.

Abstract: A computational device detects an emotion of a reader while reading a printed version of a book via a virtual reality device. A determination is made of notes made by other readers of the book with a similar emotion to the reader, where the notes have been stored in association with an electronic version of the book. The notes are displayed on a virtual reality interface of the virtual reality device to the reader.

Abstract: The present invention relates to a process for producing succinic anhydride by maleic anhydride hydrogenation, comprising (1) feeding a maleic anhydride solution and a hydrogen raw material respectively to a first-stage hydrogenation reactor from an upper liquid-phase feed port and a top gas-phase feed port of the first-stage hydrogenation reactor for a first-stage hydrogenation reaction to obtain a first-stage hydrogenation product; (2) feeding the first-stage hydrogenation product to a second-stage hydrogenation reactor for a second-stage hydrogenation reaction to obtain a second-stage hydrogenation product, optionally, before entering the second-stage hydrogenation reactor, subjecting the first-stage hydrogenation product to a first-stage gas-liquid separation to obtain a first-stage gas phase and a first-stage liquid phase, and then feeding the first-stage gas phase and the first-stage liquid phase respectively from a top gas-phase feed port and an upper liquid-phase feed port of the second-stage hydrogen

Abstract: A nanopore measurement circuit includes a first analog memory configured to store a first electrical value corresponding to a first measurement sample of a nanopore and a second analog memory configured to store a second electrical value corresponding to a second measurement sample of the nanopore. The nanopore measurement circuit also includes a measurement circuitry configured to provide an output indicating a difference between the first electrical value of the first analog memory and the second electrical value of the second analog memory.

Abstract: Disclosed herein are coronavirus Spike (S) proteins and nanoparticles comprising the same, which are suitable for use in vaccines. The nanoparticles present antigens from pathogens surrounded to and associated with a detergent core resulting in enhanced stability and good immunogenicity. Dosages, formulations, and methods for preparing the vaccines and nanoparticles are also disclosed.

Abstract: Provided is a display panel. The display panel includes: a cutout region, a display region disposed on a periphery of the cutout region, and a winding region disposed between the cutout region and the display region. The display panel further includes: a substrate; a plurality of light-emitting devices disposed on the substrate, and a package layer and a light-shielding layer that are successively stacked and disposed on a side, distal from the substrate, of the plurality of light-emitting devices. The plurality of light-emitting devices are within the display region. The package layer is closer to the substrate relative to the light-shielding layer, and at least a portion of the light-shielding layer is within the winding region.

Abstract: Disclosed herein are methods and nanoparticles suitable for use in reducing exacerbations in COPD patients. The methods and compositions advantageously reduce the incidence of hospitalization in COPD patients that occurs in response to environmental insults such as exposure to or infection by RSV. Dosages, formulations, and methods for preparing the vaccines and nanoparticles are also disclosed.

Abstract: A system includes a circuit configured to detect a voltage corresponding to an electrical measurement of a nanopore. The system also includes a component configured to compare the voltage to another voltage. Based at least in part on the comparison, a one bit indicator is determined. The one bit indicator indicates whether the voltage indicates a change in a state of the nanopore. In the event it is determined that the voltage indicates the change in the state of the nanopore, a multiple bit signal is provided for output.

Abstract: The present disclosure provides systems and methods for analyzing or identifying a target nucleic acid molecule. For example, a system of the present disclosure may comprise (a) a complex comprising a target nucleic acid molecule and a primer nucleic acid molecule, wherein the primer nucleic acid molecule comprises (i) a region that is complementary to a portion of the target nucleic acid molecule and (ii) an additional region that is non-complementary the target nucleic acid molecule, and (b) with said additional region having flown through a pore of a sensor, using said sensor to identify said additional region, thereby analyzing said target nucleic acid molecule. The sensor may be configured to detect one or more signals indicative of an impedance or impedance change in the sensor when the at least the portion of the target molecule is bound by the binding unit. The one or more signals may be usable to analyze or identify the target molecule.

Abstract: The present disclosure provides methods for processing a nucleic acid sample. For example, a method of the present disclosure may simultaneously process two populations of nucleic acid (NA) templates for two different sequencing modalities: (i) a first population comprising circularized NA templates having a size smaller than a size threshold (e.g., smaller than 2 kilobase (kb)), and (ii) a second population comprising uncircularized NA templates having a size greater than or equal to the size threshold.

Abstract: Disclosed herein are methods and nanoparticles suitable for use in reducing exacerbations in COPD patients. The methods and compositions advantageously reduce the incidence of hospitalization in COPD patients that occurs in response to environmental insults such as exposure to or infection by RSV. Dosages, formulations, and methods for preparing the vaccines and nanoparticles are also disclosed.

Abstract: A wireless federated learning (FL) framework and a resource optimization method are provided to resolve a problem that FL is not suitable for many hardware-constrained Internet of Things (IoT) devices with a small amount of computing resources. In the framework, users with sufficient computing resources upload locally trained model parameters to a base station, and users with limited computing resources only need to send training data to the base station. The base station performs data training and model aggregation to obtain a global model. In this way, the users with limited computing resources and the users with sufficient computing resources cooperatively train the global model. To improve a data transmission rate and reduce an aggregation error of FL, a non-convex optimization problem is constructed to jointly design user transmit power and a reception strategy of the base station, and solves the problem through a successive convex approximation (SCA) method.

Abstract: A multi-functional reconfigurable intelligence surface (MF-RIS) integrating signal reflection, refraction and amplification and energy harvesting and an application thereof are provided. The MF-RIS can support wireless signal reflection, refraction and amplification and energy harvesting on one surface, to amplify, reflect, or refract a signal through harvested energy, and further enhance effective coverage of wireless signals. When a signal model of the MF-RIS constructed in the present disclosure is applied to a multi-user wireless network, a non-convex optimization problem of jointly designing operation modes and parameters that include BS transmit beamforming, and different components and a deployment position of the MF-RIS is constructed with an objective of maximizing a sum rate (SR) of a plurality of users in an MF-RIS-assisted non-orthogonal multiple access network.

Abstract: Disclosed herein are multivalent nanoparticle vaccine compositions suitable for use in influenza vaccines. The nanoparticles include effective amounts of influenza glycoproteins that provide increased immune responses compared to a commercially available influenza vaccine composition. The present disclosure also provides vaccine formulation strategies that are cost effective and are convenient for clinical use. Methods of administering the nanoparticle vaccine compositions to a subject are also disclosed.

Abstract: Provided are a chassis ear apparatus and a mounting method thereof. The chassis ear apparatus includes a shell, wherein a chassis ear seat is detachably mounted on a back surface of the shell, and the chassis ear seat is configured to be fixed to a rack after a server is installed into the rack; a printed circuit board is arranged at a front surface of the shell, the printed circuit board is installed from the front surface of the shell and is detachably mounted on the chassis ear seat, and the printed circuit board is connected to a mainboard inside the server via a signal cable; and the signal cable penetrates through a gap between the shell and the chassis ear seat and is inserted into a cable socket on a front surface of the printed circuit board from the front surface of the shell.

Abstract: A semi-federated learning (semiFL) method based on a next-generation multiple access (NGMA) technology is provided. Centralized learning (CL) and FL are integrated such that devices with weak computing capabilities can also participate in training of a global model. A simultaneously transmitting and reflecting reconfigurable intelligent surface (STAR-RIS) is deployed to dynamically change a channel environment such that a system can meet different task requirements of heterogeneous users. Communication-centric CL users and computing-centric FL users can transmit data in parallel on a same time-frequency resource. This avoids a waste of data resources, enriches data obtaining of a base station (BS), and improves accuracy of the global model. The semiFL method also integrates a strategy for jointly optimizing user power allocation and a configuration of the STAR-RIS to reduce total uplink transmit power consumption of the system and prolong a life cycle of an intelligent Internet of Things (IoT) network.

Abstract: Disclosed herein are coronavirus Spike (S) proteins and nanoparticles comprising the same, which are suitable for use in vaccines. The nanoparticles present antigens from pathogens surrounded to and associated with a detergent core resulting in enhanced stability and good immunogenicity. Dosages, formulations, and methods for preparing the vaccines and nanoparticles are also disclosed.

Abstract: A high-power pulsed surface processing system includes insulated-gate bipolar transistors (IGBT) to replicate desirable pulse structures with high precision, at low cost, and with high reliability within a single system. The pulsed surface processing system includes a power supply, an anode and a cathode, a dual gate driver supplying power to one or more IGBT gates, and one or more capacitor banks. Pulse formation software controls the timing and duration of electrical pulses to the electrodes. A freewheeling diode protects the system from an abrupt reduction of current in the circuit. The high-power pulsed surface processing system may be used to control versatile and complex pulse structures while with precise control of instantaneous pulse powers, pulse timing, and process control. The inclusion of dual gate drivers also offers the ability for multiple pulsers to be created and “slaved” together for a wide variety of custom processes.

Abstract: Disclosed herein are methods and compositions for treating or preventing bacterial infection. In particular, the methods and compositions are directed towards C. difficile infection. In particular aspects, the compositions are vaccines containing multimeric polypeptides containing portions of multiple toxins from bacteria. The polypeptides induce effective immune responses thus treating or preventing infection.