Abstract: The present invention relates to a CRISPR-Cas13 system and use thereof, and also relates to a Cas13 protein, a fusion protein, and a guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared to SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. The guide polynucleotide comprises a same-direction repetition sequence and a guide sequence that has been engineered to hybridize with the target RNA. The same-direction repetition sequence has at least 70% sequence identity to any of SEQ ID NOs: 3 and 80-87. The CRISPR-Cas13 system comprises the Cas13 protein that has at least 90% sequence identity to SEQ ID NO: 1, or a coding nucleic acid therefor, and the guide polynucleotide or a coding nucleic acid therefor.

Abstract: Compositions and methods provide a health benefit to a growing animal by administering a food composition to the animal, and the food composition includes a brain development blend. The brain development blend generally contains DHA, EPA, taurine, choline, thiamine, riboflavin, pantothenic acid, niacin, pyridoxine, vitamin B12, and folic acid.

Abstract: Provided are an information receiving method and apparatus, an information transmitting method and apparatus, and a storage medium. The information receiving method includes: A UE in a RRC_UNCONNECTED state receives sub-grouping configuration information and PEI configuration information by means of a system broadcast message, wherein a UE that has joined a MBS session does not detect a PEI, and detects a paging message at a paging occasion; and a UE that has not joined the MBS session detects a PEI at a PEI occasion according to the sub-grouping configuration information and the PEI configuration information; and if the PEI indicates that a paging message of sub-grouping to which the UE belongs exists, the UE detects the paging message at the paging occasion.

Abstract: Provided are a cell reselection method, apparatus, and system for sidelink communication. The cell reselection method for sidelink communication includes the following. A UE in an inactive state and participating in the sidelink communication receives cell reselection information, where the cell reselection information includes cell reselection information for implementing service continuity of the sidelink communication. The UE performs cell reselection according to the cell reselection information and capability information of the UE.

Abstract: The disclosed concepts relate to employing agent behavior models to control agent behavior in an application, such as a video game or a simulation. For instance, in some implementations, agent behavior models with relatively greater resource utilization, such as generative language models, are assigned to agents that are at higher levels of an agent hierarchy. Agent behavior models with relatively less resource utilization, such as reinforcement learning or hard-coded models, are assigned to agents that are at lower levels of the agent hierarchy.

Abstract: The disclosure discloses an AI-based condition classification system for patients with novel coronavirus, which includes: a classification model acquisition module for training one or more binary classification models that classify patient conditions according to patient data, and obtain the most accurate binary classification model as the target model, and determine the interpretable features in the patient data; a preprocessing module is configured to extract the interpretable features in the patient data to be classified, and then perform preprocessing on the extracted features to fill in the missing values and replace the abnormal values in the data, so as to obtain the features to be classified after the preprocessing is completed; a condition classification module is configured to use the features to be classified as the input for the target model, and the target model is used to complete condition classification for the patients to be classified.

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

Abstract: Provided are a novel Cas effector protein, a gene editing system, and uses thereof. The Cas effector protein is CasRfg.3 and similar proteins. The CasRfg.3 (also referred to as Ca2) protein of the present disclosure has a relatively shorter amino acid sequence compared to the commonly used SpCas9 protein, allowing it to be easily packaged into small-capacity gene therapy vectors. The CasRfg.3 corresponds to a unique PAM sequence. Additionally, the CasRfg.3 exhibits excellent specificity in targeting and editing nucleic acid sequences, shows good adaptation to temperature variations, and has a wide temperature tolerance range, functioning effectively under high-temperature conditions, such as being capable of cleaving or modifying target nucleic acids at temperatures ranging from 25° C. to 55° C. The novel Cas effector protein of the present disclosure has significant application value in fields like gene therapy.

Abstract: A method of mimicking health benefits of physical exercise in a cat can include administering a high protein diet to a cat, the high protein diet including from about 110% to about 350% of a Recommended Daily Allowance (RDA) of protein for the cat on a daily basis. The high protein diet in this example is sufficient to increase a 12,13-diHOME blood level for an 18-24 hour period per day compared to a baseline blood level without the need for physical exercise.

Abstract: A method of mimicking health benefits of physical exercise or voluntary cold exposure, e.g., cold plunge, in an animal can include administering to the animal a high protein diet including from about 150% to about 400% of a Recommended Daily Allowance (RDA) of protein for the animal on a daily basis. The high protein diet can be sufficient to increase a 12,13-diHOME blood level for an 18-24 hour period per day compared to a baseline blood level without the need for the physical exercise or voluntary cold exposure.

Abstract: A polyacrylamide-based symmetric branched polymer surfactant and a process for preparing the same and use thereof are provided. The surfactant has an acrylamide structure unit, an anionic monomer structure unit, an optional non-ionic monomer structure unit, a branched structure unit, and an optional cationic monomer structure unit. The branched structure unit has at least one of structures represented by formula (1) or formula (2): The polymer surfactant can realize both the high viscosity of the aqueous solution and the low oil water interfacial tension, and can be used as the oil displacement agent in the tertiary oil recovery.

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

Abstract: Tissue product compositions and methods for treating a patient are provided. The tissue product composition may include a flowable carrier including a hyaluronic acid based material and acellular tissue matrix particles mixed within the carrier. Methods of producing the tissue product composition and an injection device filled with the tissue product composition are also provided.

Abstract: A system, a method, and a composition for targeted gene manipulation and use thereof are provided. The system specifically targets an RNA molecule encoding an ApoE4 protein, which may be used to treat diseases such as Alzheimer's disease.

Abstract: The present invention relates to a CRISPR-Cas13 system and use thereof, and also relates to a Cas13 protein, a fusion protein, and a guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared to SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. The guide polynucleotide comprises a same-direction repetition sequence and a guide sequence that has been engineered to hybridize with the target RNA. The same-direction repetition sequence has at least 70% sequence identity to any of SEQ ID NOs: 3 and 80-87. The CRISPR-Cas13 system comprises the Cas13 protein that has at least 90% sequence identity to SEQ ID NO: 1, or a coding nucleic acid therefor, and the guide polynucleotide or a coding nucleic acid therefor.

Abstract: Provided is a subject matter relates to a process for producing fermentation products from starch-containing material comprising the steps of: i) saccharifying using a carbohydrate-source generating enzyme; ii) fermenting using a fermenting organism; wherein at least one polypeptide having cellobiohydrolase activity, endoglucanase activity, or beta-glucosidase activity are present or added during fermentation or simultaneous saccharification and fermentation. The subject matter also relates to an enzyme blend or composition comprising the polypeptides.

Abstract: A user terminal positioning method is used in an edge cloud server, and includes: receiving satellite positioning information sent by a user terminal; determining a location service area in which the user terminal is located; on the basis of the location service area, acquiring a differential correction model corresponding to the location service area from a public cloud server; using the satellite positioning information and the differential correction model to implement location calculation to obtain location information of the user terminal; and sending the location information to the user terminal.

Abstract: The present invention relates to isolated polypeptides having beta-glucanase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules. The present invention further relates to processes for producing fermentation products from starch-containing or cellulosic-containing material, as well as an enzyme blend or composition, or a recombinant host cell or fermenting organism suitable for use in processes of the invention.

Abstract: Disclosed are a doped colloidal metal oxide quantum wire and a preparation method thereof, a gas sensor, a gas sensor array, and use thereof, which relate to the technical field of gas sensors. In the preparation method, a main metal precursor, a doped metal precursor, an alcohol solvent, and a fatty acid surfactant are mixed, and a resulting mixture is subjected to a solvothermal reaction, to obtain the doped colloidal metal oxide quantum wire, wherein a main metal element in the main metal precursor is selected from Sn, Zn, W, In, Mo, Fe, Co, Ni, and Cu; a doped metal element in the doped metal precursor is selected from V, Nb, Ta, Cr, Mo, W, Mn, Fe, Co, Ni and Cu; the doped metal element is different from the main metal element; and the solvothermal reaction is performed at a temperature of 150-300° C.

Abstract: A fuel ejector assembly includes a nozzle structured to receive fuel from a fuel conduit and eject the fuel therethrough, and an exhaust gas recirculation (“EGR”) conduit structured to communicate a recirculated exhaust gas therethrough. A mixing portion is disposed downstream of the nozzle and the EGR conduit, the nozzle and the EGR conduit fluidly coupled to the mixing portion such that the mixing portion receives each of the fuel and the recirculated exhaust gas. A diffuser is disposed downstream of the mixing portion and is configured to be fluidly coupled to an engine to communicate a mixture of the fuel and the recirculated exhaust gas to the engine.