Patents by Inventor Ichiro Sase

Ichiro Sase has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190137754
    Abstract: An analysis device for quantifying a state of a fluorescent image containing a plurality of bright spots comprises an area setting unit in which states of a plurality of bright spots contained in a plurality of areas set in the fluorescent image in accordance with positions of the plurality of bright spots are quantified as numerical values.
    Type: Application
    Filed: January 4, 2019
    Publication date: May 9, 2019
    Applicant: NIKON CORPORATION
    Inventor: Ichiro SASE
  • Patent number: 10268033
    Abstract: An analysis device for quantifying a state of a fluorescent image containing a plurality of bright spots comprises an area setting unit in which states of a plurality of bright spots contained in a plurality of areas set in the fluorescent image in accordance with positions of the plurality of bright spots are quantified as numerical values.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: April 23, 2019
    Assignee: NIKON CORPORATION
    Inventor: Ichiro Sase
  • Publication number: 20180356321
    Abstract: A sample preparation method includes: irradiating a first region of a sample with light at a time t1; irradiating a second region different from the first region with the tight at a time t2 after the time t1; and fixing the sample at a time t3 after the time t2. A sample preparing apparatus includes: a light radiating unit that irradiates a first region of a sample with light at time t1 and irradiates a second region different from the first region with the light at a time t2 after the time t1; and a fixing unit that fixes the sample at a time t3 after the time t2.
    Type: Application
    Filed: August 20, 2018
    Publication date: December 13, 2018
    Applicant: NIKON CORPORATION
    Inventor: Ichiro SASE
  • Publication number: 20180299659
    Abstract: Provided is a microscope including: an illumination optical system that irradiates a specimen including a fluorescent substance with illumination light; an observation optical system including an objective lens; an imager that takes an image formed by the observation optical system; an image processor that performs image processing by using a result of the image taking; and a controller that moves at least one of a stage and the objective lens in the optical axis direction of the objective lens.
    Type: Application
    Filed: May 29, 2018
    Publication date: October 18, 2018
    Applicant: NIKON CORPORATION
    Inventors: Ichiro SASE, Yasutoshi KANEKO, Tatsuo FUKUI
  • Publication number: 20180275061
    Abstract: A microscope apparatus including: an illumination optical system that radiates activation light to activate some of fluorescent materials included in a sample and excitation light to excite at least some of the activated fluorescent materials; an image forming optical system that: has an objective lens and an astigmatic optical system that generates astigmatism to at least part of fluorescence from the fluorescent materials; and forms an image of the fluorescence; an image-capturing unit that captures an image formed by the image forming optical system; a drive unit that moves an image-capturing position in the sample along an optical axis-direction of the objective lens; and a control unit, wherein the control unit causes the image-capturing unit to capture images in a plurality of numbers of frames respectively at a first image-capturing position and at a second image-capturing position different from the first image-capturing position.
    Type: Application
    Filed: May 29, 2018
    Publication date: September 27, 2018
    Applicant: NIKON CORPORATION
    Inventors: Ichiro SASE, Yasutoshi KANEKO, Tatsuo FUKUI
  • Patent number: 9946058
    Abstract: A microscope apparatus comprises: a distribution measurement apparatus which—with respect to an observation region wherein a sample is arranged which comprises a fluorescent material that is activated when irradiated with an activating light of a prescribed wavelength, and that emits fluorescence when irradiated in an activated condition with an exciting light of a wavelength that differs from that of the activating light—obtains a fluorescent picture image by conducting irradiation with the exciting light, and measures a fluorescent intensity distribution of the observation region; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by multiply repeating operations comprising an operation wherein the observation region is irradiated with the activating light at th
    Type: Grant
    Filed: June 10, 2011
    Date of Patent: April 17, 2018
    Assignee: NIKON CORPORATION
    Inventor: Ichiro Sase
  • Publication number: 20160202465
    Abstract: An analysis device for quantifying a state of a fluorescent image containing a plurality of bright spots comprises an area setting unit in which states of a plurality of bright spots contained in a plurality of areas set in the fluorescent image in accordance with positions of the plurality of bright spots are quantified as numerical values.
    Type: Application
    Filed: March 24, 2016
    Publication date: July 14, 2016
    Applicant: NIKON CORPORATION
    Inventor: Ichiro SASE
  • Patent number: 8629382
    Abstract: An reference light emitted from an LED for auto focus enters a glass cover, on which a sample is adhered to, via a half mirror to an objective lens. The reference light that entered the glass cover is reflected by the boundary surface to be reflected light, and this reflected light enters a dichroic mirror via an objective lens. A part transmits the reflected light and allows the light to enter the camera via the dichroic mirror to the lens. A user rotates a motor-operated mirror while viewing the image of the reference light captured by a camera, so as to shift the reference light image position on the boundary surface.
    Type: Grant
    Filed: August 29, 2008
    Date of Patent: January 14, 2014
    Assignee: Nikon Corporation
    Inventor: Ichiro Sase
  • Patent number: 8507261
    Abstract: A laboratory dish comprises an incubation container, containing cultured cells, for observation through a microscope, in which a partitioning member partitions an interior of the incubation container into a plurality of incubation chambers by substantially perpendicular walls. The partitioning member tightly fits to a bottom portion of the incubation container, thereby capable of retaining in separation a liquid inputted in each of the incubation chambers from liquids in the other incubation chambers. A cover member covers an upper portion of each of the incubation chambers partitioned by the partitioning member and tightly fitted to the partitioning member. A whole or part of the cover member is formed of a transparent member, and the upper portion of each incubation chamber is formed with an opening via which to input the liquid.
    Type: Grant
    Filed: November 29, 2009
    Date of Patent: August 13, 2013
    Assignee: Nikon Corporation
    Inventors: Yasushi Ogihara, Ichiro Sase, Roman Zantl
  • Patent number: 8208202
    Abstract: A focus detection apparatus is provided with a light source 16 that emanates light with a given wavelength range; a reflection member 15 that reflects light emanated from the light source 16 to lead to an object 6a, reflects light from the light source 16 reflected from the object 6a, and transmits light from the object 6a with at least two different wavelength ranges except the light reflected from the object 6a; a photodetector 21 detecting the light from the light source 16 reflected from the object 6a; and a controller 22 detecting a focus shift between the objective lens 8 and the object 6a in the microscope 2 based on a signal detected by the photodetector 21, thereby providing a focus detection apparatus capable of limiting wavelength range of light for focus detection thereby able to use wider wavelength range for the microscope observation, and a microscope equipped therewith.
    Type: Grant
    Filed: July 9, 2009
    Date of Patent: June 26, 2012
    Assignee: Nikon Corporation
    Inventor: Ichiro Sase
  • Publication number: 20120062722
    Abstract: A microscope apparatus comprises: a distribution measurement apparatus with respect to an observation region that comprises a fluorescent material that is activated when irradiated with an activating light, and that emits fluorescence when irradiated with an exciting light of a wavelength different from the activating light, obtains a fluorescent picture image through irradiation with the exciting light, and measures a fluorescent intensity distribution; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by repeating an operation wherein the observation region is irradiated with the activating light at the set irradiation intensities, and repeating an operation wherein a fluorescent picture image is obtained by irradiating with the exciting light after the activat
    Type: Application
    Filed: June 10, 2011
    Publication date: March 15, 2012
    Applicant: NIKON CORPORATION
    Inventor: Ichiro SASE
  • Patent number: 8098279
    Abstract: Providing an imaging apparatus and a microscope capable of taking two-dimensional images of a sample at a plurality of observation positions different in the optical axis direction at the same time. The apparatus includes an image-forming lens 15 that forms images of a sample 4 on a plurality of image-forming places; an optical-path-dividing member 17, 18, 19 that divides an optical path from the same area in a plane perpendicular to an optical axis of the sample 4 so as to form the plurality of image-forming places; and an optical-path-length-changing member 27, 28, 29 that is provided on at least one optical path between the plurality of image-forming places and the imaging lens 15.
    Type: Grant
    Filed: April 23, 2009
    Date of Patent: January 17, 2012
    Assignee: Nikon Corporation
    Inventors: Ichiro Sase, Toshiaki Nihoshi
  • Patent number: 7957057
    Abstract: An observation field of view of a microscope can be moved without moving or changing an objective lens and without varying position or state of a sample. A microscope optical system has a mirror that changes the direction of the optical path by reflection and is located in the optical path between an objective lens of the microscope and an image to be observed. The mirror is able to be tilted by changing the position of a reflecting surface of the mirror. Accordingly, the observation field of view is moved by tilting the mirror. In other words, the observation field of view can be moved without changing a positional relationship between the objective lens of the microscope and the sample.
    Type: Grant
    Filed: August 5, 2009
    Date of Patent: June 7, 2011
    Assignee: Nikon Corporation
    Inventors: Ichiro Sase, Katsuya Watanabe
  • Publication number: 20110127406
    Abstract: An reference light emitted from an LED for auto focus enters a glass cover, on which a sample is adhered to, via a half mirror to an objective lens. The reference light that entered the glass cover is reflected by the boundary surface to be reflected light, and this reflected light enters a dichroic mirror via an objective lens. A part transmits the reflected light and allows the light to enter the camera via the dichroic mirror to the lens. A user rotates a motor-operated mirror while viewing the image of the reference light captured by a camera, so as to shift the reference light image position on the boundary surface.
    Type: Application
    Filed: August 29, 2008
    Publication date: June 2, 2011
    Applicant: NIKON CORPORATION
    Inventor: Ichiro Sase
  • Patent number: 7864414
    Abstract: Providing a microscope capable of movably adjusting an observation field of a sample without moving the sample. The microscope includes a first objective lens, a second objective lens, a mirror, an angular adjustment mechanism, and a shift mechanism. The first objective lens is disposed to the sample side. The second objective lens forms an intermediate image of the sample together with the first objective lens. The mirror is disposed with a tilt on an optical path between the first objective lens and the second objective lens. The angular adjustment mechanism rotatably adjust the mirror in the tilt direction. The shift mechanism makes a shift adjustment of the second objective lens in an axial direction of a rotation axis of the mirror. With the configuration, the observation field can be moved two-dimensionally by the angular adjustment mechanism.
    Type: Grant
    Filed: March 24, 2009
    Date of Patent: January 4, 2011
    Assignee: Nikon Corporation
    Inventors: Ichiro Sase, Shuji Toyoda
  • Publication number: 20100208052
    Abstract: A user operates an operating section to cause a motorized stage to move, and observes and checks an entire sample that is disposed on the motorized stage. When the motorized stage is stopped for a predetermined reference time or longer, a controller recognizes the position where the stage is stopped as a position for time-lapse observation, and records as observation position information that observation position. After observation of the entire sample, the controller sequentially moves the motorized stage to the observation positions indicated by the observation position information, on the basis of the recorded observation position information, whereby the user observes the sample at each observation position and decides on a final observation position.
    Type: Application
    Filed: August 29, 2008
    Publication date: August 19, 2010
    Applicant: NIKON CORPORATION
    Inventor: Ichiro Sase
  • Publication number: 20100136671
    Abstract: A laboratory dish comprises an incubation container, containing cultured cells, for observation through a microscope, in which a partitioning member partitions an interior of the incubation container into a plurality of incubation chambers by substantially perpendicular walls. The partitioning member tightly fits to a bottom portion of the incubation container, thereby capable of retaining in separation a liquid inputted in each of the incubation chambers from liquids in the other incubation chambers. A cover member covers an upper portion of each of the incubation chambers partitioned by the partitioning member and tightly fitted to the partitioning member. A whole or part of the cover member is formed of a transparent member, and the upper portion of each incubation chamber is formed with an opening via which to input the liquid.
    Type: Application
    Filed: November 29, 2009
    Publication date: June 3, 2010
    Inventors: Yasushi Ogihara, Ichiro Sase, Roman Zantl
  • Publication number: 20100002289
    Abstract: The purpose is to move an observation field of view of a microscope without moving or changing an objective lens without varying position or state of a sample. A microscope optical system according to the present invention has a mirror that changes the direction of the optical path by reflection and locates in the optical path between an objective lens of the microscope and an image to be observed. The mirror is able to be tilted with changing the position of a reflecting surface of the mirror. Accordingly, the observation field of view is moved by tilting the mirror. In other words, the observation field of view can be moved without changing positional relation between the objective lens of the microscope and the sample.
    Type: Application
    Filed: August 5, 2009
    Publication date: January 7, 2010
    Inventors: Ichiro Sase, Katsuya Watanabe
  • Patent number: 7630113
    Abstract: An observation field of view of a microscope can be moved without moving or changing an objective lens and without varying position or state of a sample. A microscope optical system has a mirror that changes the direction of the optical path by reflection. The mirror is located in the optical path between an objective lens of the microscope and an image to be observed. The mirror can be tilted to change the position of a reflecting surface of the mirror. Accordingly, the observation field of view is moved by tilting the mirror without changing a positional relation between the objective lens of the microscope and the sample.
    Type: Grant
    Filed: May 5, 2005
    Date of Patent: December 8, 2009
    Assignee: Nikon Corporation
    Inventors: Ichiro Sase, Katsuya Watanabe
  • Publication number: 20090273830
    Abstract: A focus detection apparatus is provided with a light source 16 that emanates light with a given wavelength range; a reflection member 15 that reflects light emanated from the light source 16 to lead to an object 6a, reflects light from the light source 16 reflected from the object 6a, and transmits light from the object 6a with at least two different wavelength ranges except the light reflected from the object 6a; a photodetector 21 detecting the light from the light source 16 reflected from the object 6a; and a controller 22 detecting a focus shift between the objective lens 8 and the object 6a in the microscope 2 based on a signal detected by the photodetector 21, thereby providing a focus detection apparatus capable of limiting wavelength range of light for focus detection thereby able to use wider wavelength range for the microscope observation, and a microscope equipped therewith.
    Type: Application
    Filed: July 9, 2009
    Publication date: November 5, 2009
    Inventor: ICHIRO SASE