Abstract: A method and a composition for delivery of a biomaterial to an animal cell or a tissue, the composition includes (a) a biomaterial; (b) a biodegradable cross-linker portion having a hydrolyzable bond, wherein the biodegradable cross-linker portion is covalently bound to the biomaterial; and (c) a substrate, wherein the substrate is covalently bound to the biodegradable cross-linker portion, provided that the biodegradable cross-linker is adapted to hydrolyze by breaking the hydrolyzable bond and thereby release and deliver the biomaterial. A process of making the composition is also provided.

Abstract: A device having polymeric filaments, wherein at least one of the filaments includes at least one groove for slidably retaining at least one other filament, such that the device is adapted to revert to a tubular lattice structure when allowed to expand from a collapsed state. A device as described above and further including a biologically active function, wherein the polymeric filaments of the device include an agent having a reactive group or a fiber adapted to covalently react with a biomaterial. Thus, the device of the invention has an active structural function such as the ability to regain a shape and, optionally, a biologically active function such as the ability to deliver a biomaterial to an organism or a cell. A process of manufacturing the device is also described.

Abstract: The present invention relates to compositions and methods of immobilizing a viral vector to an implantable medical device, for example a vascular stent. Specifically, a composition for delivery of a therapeutic agent is provided which includes: a gene transfer vector, a surface and a modified protein, wherein the gene transfer vector is bound to the modified protein and the modified protein is covalently bound to the surface and wherein the composition is adapted to deliver the gene transfer vector to a mammalian cell. The viral vector is preferably an adenoviral vector and the modified protein is preferably CAR D1.

Abstract: A water-soluble photo-activatable polymer including: a photo-activatable group adapted to be activated by an irradiation source and to form a covalent bond between the water-soluble photo-activatable polymer and a matrix having at least one carbon; a reactive group adapted to covalently react with a biomaterial for subsequent delivery of the biomaterial to a cell; a hydrophilic group; and a polymer precursor. A composition including a monomolecular layer of the water-soluble photo-activatable polymer and a matrix having at least one carbon, wherein the monomolecular layer is covalently attached to the matrix by a covalent bond between the photo-activatable group and the at least one carbon. The composition further includes a biomaterial having a plurality of active groups, wherein the biomaterial is covalently attached to the monomolecular layer by covalent bonding between the active groups and reactive groups. Also provided is a method for delivery of a biomaterial to a cell.

Abstract: A particle including a matrix-forming agent and a polyelectrolyte-amphiphilic agent adduct wherein the polyelectrolyte-amphiphilic agent adduct is in physical communication with the matrix-forming agent. The particle further includes a coated magnetic field-responsive agent and a biomaterial. Methods of making the particle are provided. Also provided are methods of delivery of the biomaterial to a target cell or a target tissue including administering the particle having the matrix-forming agent, polyelectrolyte-amphiphilic agent adduct, the coated magnetic field-responsive agent and the biomaterial; providing a magnetic device associated with the target cell or the target tissue; applying a magnetic force to the particle; and guiding the particle toward the magnetic device by the magnetic force.

Abstract: A process for preparing the polyurethane derivative, the process including: providing a polyurethane having a urethane amino moiety, providing a multifunctional linker reagent of a formula: LG-RL-(FG)n, wherein n is an integer from 1 to 3, FG is a functional group, which is a halogen, a carboxyl group, a sulfonate ester, or an epoxy group, LG is a leaving group, which is a halogen, a carboxyl group, a sulfonate ester, or an epoxy group, and RL is an (n+1)-valent organic radical having at least one carbon atom; providing a protected thiol-containing reagent of a formula R—C(O)SH, or a salt thereof, wherein R is a C1 to C6 alkyl group; reacting the multifunctional linker reagent with the urethane amino moiety to form a polyurethane substituted with at least one substituent group of a formula: —RL-(FG)n; reacting the polyurethane with a protected thiol-containing reagent to form the polyurethane derivative.

Abstract: A polyurethane having a thiol substituent pendant from at least one nitrogen and/or at least one carbon atom of the polyurethane. A polyurethane having a thiol substituent pendant from at least one nitrogen and/or at least one carbon atom of the polyurethane, wherein the thiol substituent has a formula: —RL—(S—R1)n wherein n is an integer from 1 to 3; RL is an (n+1)-valent organic radical having at least one carbon atom; and R1 is a member selected from the group consisting of H, C(O)R3, and SR4, wherein R3 is a member selected from the group consisting of C1 to C6 alkyl and R4 is a heterocyclic group or an electron deficient aromatic group.

Abstract: A polyurethane having a thiol substituent pendant from at least one nitrogen and/or at least one carbon atom of the polyurethane.

Abstract: A process for preparing the polyurethane derivative, the process including: providing a polyurethane having a urethane amino moiety, providing a multifunctional linker reagent of a formula: LG-RL-(FG)n, wherein n is an integer from 1 to 3, FG is a functional group, which is a halogen, a carboxyl group, a sulfonate ester, or an epoxy group, LG is a leaving group, which is a halogen, a carboxyl group, a sulfonate ester, or an epoxy group, and RL is an (n+1)-valent organic radical having at least one carbon atom; providing a protected thiol-containing reagent of a formula R—C(O)SH, or a salt thereof, wherein R is a C1 to C6 alkyl group; reacting the multifunctional linker reagent with the urethane amino moiety to form a polyurethane substituted with at least one substituent group of a formula: —RL-(FG)n; reacting the polyurethane with a protected thiol-containing reagent to form the polyurethane derivative.

Abstract: A method of determining a binding capacity of a surface, the method including providing the surface containing a reactive moiety; providing a fluorophore including a fluorescent moiety adapted to emit a detectable signal; reacting the fluorophore with the reactive moiety to form a linking bond between the fluorophore and the reactive moiety; cleaving a cleavable bond to liberate the fluorescent moiety; and detecting the detectable signal to determine the binding capacity of the surface.