Abstract: Disclosed is a method for producing soluble CRM197 protein at high yield by overexpressing the protein in the cytoplasm of E. coli. The method includes a step of culturing recombinant E. coli harboring an expression plasmid carrying a gene sequence coding for a recombinant CRM197 protein under a condition suitable for expression of the recombinant CRM197 protein which has a histidine-tag attached to the C-terminus of CRM197 and a maltose-binding protein (MBP) attached to the N-terminus of CRM197. The method further includes a step of purifying after the culturing, wherein the step of purifying comprises a step of treating with tobacco etch virus (TEV) protease to remove the maltose-binding protein and a step of removing the histidine-tag via histidine-tag affinity chromatography.

Abstract: A method of controlling a cooking apparatus, comprising a cleaning process comprising controlling a temperature in a cooking chamber such that the temperature in the cooking chamber is maintained at a preset first temperature for a preset first time, and controlling a temperature in a cooking chamber such that the temperature in the cooking chamber is maintained at a preset second temperature higher than the first temperature for a preset second time. A cooking apparatus comprising, a main body having a cooking chamber formed therein, a convection flow unit comprising a convection heater to heat air in the cooking chamber and a convection fan to circulate the air in the cooking chamber, and a metal foam installed at a periphery of the convection flow unit to collect and decompose cooking gas contained in the air in the cooking chamber.

Abstract: The present disclosure relates to a connecting core for column-beam joint, the connecting core being able to secure excellent rigidity through a simple process without welding. To this end, a connecting core for column-beam joint includes: a closed-section intermediate column; a diaphragm; and internal reinforcing members, in which slit for inserting the internal reinforcing members are formed at the diaphragm, and the internal reinforcing members inserted in the diaphragm are combined with the intermediate column. According to the present disclosure, high rigidity is secured, as compared with the related art, when a closed-section column and a beam are connected. Further, a closed-section column and a beam can be connected without welding, so the process can be shortened, connecting become easy, and quality is also uniform.

Abstract: A nicotinamide adenine dinucleotide phosphate-dependent steroid dehydrogenase-like (NSDHL) inhibitor provided according to an aspect of the present invention can be used not only as an anticancer agent capable of overcoming resistance to EGFR-targeting anticancer agents, but also advantageously in the treatment of hyperlipidemia.

Abstract: A urinary incontinence prevention clip for men includes: a clip body having an upper frame positioned so as to correspond to the upper part of male genitalia, a lower frame positioned so as to correspond to the lower part of the male genitalia, and a connection part connected to one side of the upper frame and the lower frame and providing elastic force in a direction, in which the upper frame and the lower frame are spread; cushion parts respectively provided on the inside of the upper frame and the inside of the lower frame; and locking parts formed at both ends of the clip body so as to be fastened to each other, wherein the upper frame is formed so as not to press the blood vessels of the male genitalia and the lower frame is formed so as to press the urethras of the male genitalia.

Abstract: The present disclosure relates to a connecting core for column-beam joint, the connecting core being able to secure excellent rigidity through a simple process without welding. To this end, a connecting core for column-beam joint includes: a closed-section intermediate column; a diaphragm; and internal reinforcing members, in which slit for inserting the internal reinforcing members are formed at the diaphragm, and the internal reinforcing members inserted in the diaphragm are combined with the intermediate column. According to the present disclosure, high rigidity is secured, as compared with the related art, when a closed-section column and a beam are connected. Further, a closed-section column and a beam can be connected without welding, so the process can be shortened, connecting become easy, and quality is also uniform.

Abstract: The present invention relates to a holder for a vehicle, which can stably hold various portable devices, such as a cell phone and IT goods, by being firmly fixed to a grille of an air blowing hole by enabling four fixing bars to mechanically rotate according to the forward and backward movement of a pressurizing member such that one ends of the four fixing bars gather together or are separated from each other.

Abstract: The present invention relates to novel heterocyclic compounds useful in preparing drugs for the treatment of diseases associated with various functions of the histamine 4 receptor. Specifically, these drugs are useful in the prevention or treatment of inflammatory disorder, allergy, pain, nasal polyps, rhinitis, chronic sinusitis, nasal congestion, nasal itch, asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis, eczema, pruritus, itch skin, urticaria, idiopathic chronic urticaria, scleroderma, conjunctivitis, keratoconjunctivitis, ocular inflammation, dry eye, age-related macular degeneration, cardiac dysfunction, arrhythmia, atherosclerosis, multiple sclerosis, inflammatory bowel disease (colitis, Crohn's disease, ulcerative colitis), inflammatory pain, neuropathic pain, osteoarthritic pain, autoimmune thyroid disease, immune-mediated (also known as type I) diabetes, lupus, post-operative adhesions, vestibular disorders and cancer.

Abstract: A medical sterilizer for sterilizing a dental handpiece is disclosed. The medical sterilizer according to an embodiment of the present invention comprises: a transparent case made of a transparent material through which the interior can be seen and constitutes a part of the body; an opaque case formed by extending upwards of the transparent case, having a light source inside the upper end that radiates ultraviolet rays and is made of opaque material that does not allow the ultraviolet rays radiating from the above light source to pass through: a rubber stopper formed below the transparent case and containing a hole in which the dental handpiece can be inserted; and a lower portion fixing cap securing the rubber stopper to the opaque case.

Abstract: The present invention relates to a cosmetic composition for wrinkle reduction comprising, as an active ingredient, a Gynostemma pentaphyllum gypenoside fraction and, more specifically, to a cosmetic composition for wrinkle reduction, wherein the Gynostemma pentaphyllum gypenoside fraction includes at least one gypenoside selected from the group consisting of gypenoside UL1, gypenoside UL2, gypenoside UL3, gypenoside UL4, gypenoside UL5, gypenoside UL6, and gypenoside UL7.

Abstract: Provided is a composition for preventing or treating obesity including a mixed extract of Cinnamomi twigs, and more particularly, a pharmaceutical composition for preventing or treating obesity including a mixed extract of Cinnamomi twigs and Moutan root bark, a health functional food composition of feed composition for preventing or alleviating obesity including the composition, and a method of preventing or treating obesity including administering the pharmaceutical composition to an individual having obesity or a risk of developing obesity. The mixed extract of Cinnamomi twigs and Moutan root bark may inhibit fat absorption in the intestinal tract and increases in body weight causing obesity by single-dose administration, reduce lipid content in the blood, decrease weight and size of adipocytes, effectively inhibit accumulation of fat in liver tissue, and inhibit diarrhea, a serious side effect of Xenical™, by long-term administration.

Abstract: Disclosed is an acrylic adhesive, including an acrylic polymer obtained by polymerizing a mixture of about 120 parts by weight to about 250 parts by weight of acrylic monomers with about 0.1 parts by weight to about 1 parts by weight of an azo initiator, about 0.5 parts by weight to about 1 parts by weight of a filler, about 1.5 parts by weight to about 2.5 parts by weight of a crosslinking agent, and about 0.5 parts by weight to 1 parts by weight of an anti-static agent.

Abstract: A washing machine in which a first main heater is not driven while wash water stored in a first tub and wash water stored in a second tub are simultaneously heated, and a method of controlling the same. A washing machine includes: a first tub configured to store wash water; a first main heater configured to heat wash water stored in the first tub; a first sub heater configured to heat wash water stored in the first tub; a second tub configured to store wash water; and a second heater configured to heat wash water stored in the second tub, wherein a power consumption required to drive the first main heater is greater than a power consumption required to drive the first sub heater, and the first main heater is not driven while wash water stored in the first tube and wash water stored in the second tub are simultaneously heated.

Abstract: The present invention relates to Nelumbo nucifera callus having an increased content of gallic acid or an extract thereof and to a method for preparing the same. The Nelumbo nucifera callus extract according to the present invention has an excellent whitening effect by containing a large amount of gallic acid, and thus can be advantageously used as a cosmetic composition.

Abstract: A display device includes a display panel, and a first flexible printed circuit board bonded to a side surface of the display panel. The display panel includes: a first substrate; a first extension wire on a top surface of the first substrate, the top surface being parallel to a first direction and a second direction, the second direction being perpendicular to the first direction; a first recess portion at a side surface of the first substrate; and a first pad that extends from the first extension wire in a third direction perpendicular to the first and second directions, the first pad being disposed in the recess portion, and the first extension wire and the first pad comprise the same material.

Abstract: Disclosed is a method for expressing and purifying insoluble proteins of CRM197. The method includes: culturing a transformant transformed with an expression vector containing a gene encoding CRM197 protein; obtaining a culture of the transformant and disrupting the cell to recover an inclusion body; solubilizing the inclusion body; treating the solubilized inclusion body with a refolding reaction mixture and performing a refolding process; and purifying the refolded CRM197 protein by chromatography.

Abstract: The present invention provides a two-component bioink including a first solution and a second solution separately, wherein (i) the first solution includes a first biopolymer to which a first chemical functional group is introduced, and the second solution includes a second biopolymer to which a second chemical functional group able to chemically bond with the first chemical functional group is introduced; or (ii) the first solution includes a third biopolymer having a first electrostatic functional group, and the second solution includes a fourth biopolymer having a second electrostatic functional group able to physically bond with the first electrostatic functional group, a 3D biomaterial including the same, and a method for preparing the same.

Abstract: Provided is a compound represented by Formula 1 having an inhibitory activity on lysine-specific demethylase-1 (LSD1), an optical isomer, a solvate, a tautomer, or a pharmaceutically acceptable salt thereof, which is effective in preventing or treating a disease caused by abnormal activation of LSD1.

Abstract: A washing machine and a method for controlling the same are disclosed. The washing machine includes a plurality of washing devices, each of which includes a heater. Based on a target temperature of wash water heated by one heater of any one of the washing devices, the washing machine changes a target temperature setting environment of wash water heated by the remaining heater. The washing machine includes a first heater to heat wash water stored in a first tub, a second heater to heat wash water stored in a second tub, a control panel to provide a target temperature setting menu for setting a target temperature of the wash water stored in the first tub, and a controller to drive the first heater and the second heater. The controller changes the target temperature setting menu supplied from the control panel in response to the second target temperature.

Abstract: Provided are a pyrimidine compound represented by Formula 1, a method of preparing the compound, and a pharmaceutical use of the compound for the prevention or treatment of cancer.