Patents by Inventor Irina Borodina
Irina Borodina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240076329Abstract: The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.Type: ApplicationFiled: May 8, 2023Publication date: March 7, 2024Inventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Publication number: 20240043880Abstract: The present invention relates to improved methods for producing a hydrophobic compound, in particular a hydrophobic compound which is a pheromone such as an insect pheromone, in a fermentation process involving cultivation of a microorganism such as a yeast, said microorganism producing said hydrophobic compound, wherein the methods facilitate recovery of the hydrophobic compound from the fermentation broth, increase the titer of the hydrophobic compound and/or increase secretion of the hydrophobic compound from the microorganism.Type: ApplicationFiled: September 22, 2020Publication date: February 8, 2024Inventors: Bettina Lorántfy, Irina Borodina
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Publication number: 20230332096Abstract: The present invention relates to pheromone production, desaturated fatty alcohols or aldehydes or acetates using recombinant yeasts expressing acyl-CoA oxidase to shorten fatty acyl-CoA by two carbons, deleting endogenous acyl-CoA oxidase, express desaturase to introduce at least one double bond, acyl-CoA-reductase to convert fatty acyl-CoA into fatty alcohol. Further, acetyltransferase can be used to convert to desaturated acyl fatty acetate, alcohol dehydrogenase or fatty acyl oxidase to convert to desaturated fatty aldehyde.Type: ApplicationFiled: February 10, 2020Publication date: October 19, 2023Applicant: BioPhero ApSInventors: Irina Borodina, Leonie Wenning, Carina Holkenbrink, Kanchana Rueksomtawin Kildegaard, Baojian Ding, Christer Löfstedt, Karolis Petkevicius
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Patent number: 11685768Abstract: The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell using desaturases and fatty acyl-CoA reductase. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.Type: GrantFiled: June 24, 2016Date of Patent: June 27, 2023Assignee: Danmarks Tekniske UniversitetInventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Publication number: 20230135852Abstract: The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty acyl acetates and derivatives thereof, from a yeast cell.Type: ApplicationFiled: July 26, 2022Publication date: May 4, 2023Inventors: Irina Borodina, Carina Holkenbrink, Marie lnger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Publication number: 20220403428Abstract: The present disclosure relates to methods for production of 4-hydroxytryptamine and derivatives thereof in a yeast cell. Herein are also disclosed methods for production of halogenated tryptophans and derivatives thereof in a cell. Herein are also disclosed methods for production of methylated tryptamine. The disclosure also provides nucleic acid constructs and cells useful for performing the present methods.Type: ApplicationFiled: September 16, 2020Publication date: December 22, 2022Inventors: Irina Borodina, Nicholas Milne, Steven van der Hoek, Morten Otto Alexander Sommer, Jérémy Christophe Daniel Armetta, Philip Tinggaard Thomsen
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Patent number: 11434506Abstract: The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty alcohol acetates and derivatives thereof, from a yeast cell.Type: GrantFiled: December 15, 2017Date of Patent: September 6, 2022Assignee: Danmarks Tekniske UniversitetInventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Publication number: 20220220520Abstract: The present invention relates to microbial factories, in particular yeast factories, for production of ergothioneine. Also provided are methods for producing ergothioneine in a yeast cell, as well as useful nucleic acids, polypeptides, vectors and host cells.Type: ApplicationFiled: April 29, 2020Publication date: July 14, 2022Inventors: Irina BORODINA, Douglas Bruce KELL, Steven van der HOEK, Behrooz Darbani SHIRVANEHDEH
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Patent number: 11345922Abstract: The present invention relates to oleaginous yeast cells for the production of fatty alcohols and derivatives thereof, in particular desaturated fatty alcohols, desaturated fatty acyl acetates and desaturated fatty aldehydes. Also provided are methods for obtaining such compounds, which are useful in pheromone compositions.Type: GrantFiled: December 15, 2017Date of Patent: May 31, 2022Assignee: Danmarks Tekniske UniversitetInventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt
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Publication number: 20200102582Abstract: The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty acyl acetates and derivatives thereof, from a yeast cell.Type: ApplicationFiled: December 15, 2017Publication date: April 2, 2020Applicant: Danmarks Tekniske UniversitetInventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Publication number: 20190330646Abstract: The present invention relates to oleaginous yeast cells for the production of fatty alcohols and derivatives thereof, in particular desaturated fatty alcohols, desaturated fatty acyl acetates and desaturated fatty aldehydes. Also provided are methods for obtaining such compounds, which are useful in pheromone compositions.Type: ApplicationFiled: December 15, 2017Publication date: October 31, 2019Inventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt
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Patent number: 10077455Abstract: Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione-dependent dehydrogenase reaction, including medium supplementation with glutathione, may be combined with a 3HP producing metabolic pathway.Type: GrantFiled: June 12, 2014Date of Patent: September 18, 2018Assignee: TECHNICAL UNIVERSITY OF DENMARKInventors: Kanchana Rueksomtawin Kildegaard, Irina Borodina, Jochen Förster, Jens Nielsen
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Patent number: 10066245Abstract: A yeast cell having a reduced level of activity of NAD dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has at least one exogenous gene encoding NADP dependent GAPDH and/or has up-regulation of at least one endogenous gene expressing NADP dependent GAPDH, wherein combined expression of the enzymes NADP dependent GAPDH, PDC, ALD, ACS, ACC* and MCR in said host cell increases metabolic flux towards 3-HP via malonyl-CoA compared to an otherwise similar yeast cell lacking said genetic modification.Type: GrantFiled: June 12, 2014Date of Patent: September 4, 2018Assignee: TECHNICAL UNIVERSITY OF DENMARKInventors: Niels Bjerg Jensen, Irina Borodina, Yun Chen, Jerome Maury, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Jens Nielsen
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Publication number: 20180162916Abstract: The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell using desaturases and fatty acyl-CoA reductase. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.Type: ApplicationFiled: June 24, 2016Publication date: June 14, 2018Inventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Patent number: 9868966Abstract: A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalyzing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.Type: GrantFiled: October 10, 2013Date of Patent: January 16, 2018Assignee: TECHNICAL UNIVERSITY OF DENMARKInventors: Irina Borodina, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Fredrik Öberg
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Publication number: 20160230200Abstract: Recombinant microbial cells and methods for producing 5HTP, melatonin and related compounds using such cells are described. More specifically, the recombinant microbial cell may comprise exogenous genes encoding one or more of an L-tryptophan hydroxylase, a 5-hydroxy-L-tryptophan decarboxylyase, a serotonin acetyltransferase, an acetylserotonin O-methyltransferase; and means for providing tetrahydrobiopterin (THB), and can be further genetically modified to enrich one or more of tryptophan, S-adenosyl-L-methinonine and acetyl coenzyme A. Related sequences and vectors for use in preparing such recombinant microbial cells are also described.Type: ApplicationFiled: September 5, 2014Publication date: August 11, 2016Inventors: JIANGFENG ZHU, NIELS BJERG JENSEN, XIAO CHEN, JOCHEN FÖRSTER, IRINA BORODINA
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Publication number: 20160138056Abstract: A yeast cell having a reduced level of activity of NAD dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has at least one exogenous gene encoding NADP dependent GAPDH and/or has up-regulation of at least one endogenous gene expressing NADP dependent GAPDH, wherein combined expression of the enzymes NADP dependent GAPDH, PDC, ALD, ACS, ACC* and MCR in said host cell increases metabolic flux towards 3-HP via malonyl-CoA compared to an otherwise similar yeast cell lacking said genetic modification.Type: ApplicationFiled: June 12, 2014Publication date: May 19, 2016Applicant: TECHNICAL UNIVERSITY OF DENMARKInventors: Niels Bjerg JENSEN, Irina BORODINA, Yun CHEN, Jerome MAURY, Kanchana Rueksomtawin KILDEGAARD, Jochen FÖRSTER, Jens NIELSEN
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Publication number: 20160130613Abstract: Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione-dependent dehydrogenase reaction, including medium supplementation with glutathione, may be combined with a 3HP producing metabolic pathway.Type: ApplicationFiled: June 12, 2014Publication date: May 12, 2016Applicant: TECHNICAL UNIVERSITY OF DENMARKInventors: Kanchana Rueksomtawin KILDEGAARD, Irina BORODINA, Jochen FÖRSTER, Jens NIELSEN
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Publication number: 20150267228Abstract: A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalysing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.Type: ApplicationFiled: October 10, 2013Publication date: September 24, 2015Applicant: TECHNICAL UNIVERSITY OF DENMARKInventors: Irina Borodina, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Fredrik Öberg