Abstract: An enzyme-containing detergent composition having good deterbency against, especially, soiled spots in fabrics or clothes derived from a combination of sebum from human skin, a slight amount of sweat and invisible fine dust in the air is described. This detergent composition contains (I) (a) at least one olefin sulfonate having 10 to 20 carbon atoms, (b) at least one anionic surface active agent having an SO.sub.3 or SO.sub.4 group, except for said olefin sulfonate, and (c) at least one pyrophosphate and (II) at least one enzyme, the total amount of said components (a), (b) and (c) being at least 25% by weight based on the total amount of the detergent composition and the weight ratio of (a):(b):(c) being within the area enclosed by the points A, B, C and D defined in the attached triangular diagram.