Abstract: The disclosure provides modified pegRNAs comprising one or more appended nucleotide structural motifs which increase the editing efficiency during prime editing, increase half-life in vivo, and increase lifespan in a cell. Modifications include, but are not limited to, an aptamer (e.g., prequeosim-1 riboswitch aptamer or “evopreQi-1”) or a variant thereof, a pseudoknot (the MMLV viral genome pseudoknot or “Mpknot-1”) or a variant thereof, a tRNA (e.g., the modified tRNA used by MMLV as a primer for reverse transcription) or a variant thereof, or a G-quadruplex or a variant thereof. The disclosure further provides prime editor complexes comprising the modified pegRNAs and having improved characteristics and/or performance, including stability, improved cellular lifespan, and improved editing efficiency.

Abstract: A method includes receiving an input histology image, processing, using a cell classification model, the input histology image to generate one or more lymphocyte density maps within the input histology image, and performing morphological image processing on the one or more lymphocyte density maps to identify one or more TLS regions within the input histology image. Each TLS region is represented by a respective cluster of lymphocyte cells. For each corresponding TLS region of the one or more TLS regions identified in the input histology image, the method also includes extracting, from the respective cluster of lymphocyte cells, a respective set of TLS features, and processing, using a TLS classification model, the respective set of TLS features to classify the corresponding TLS region as one of a first TLS maturation state, a second TLS maturation state, or a third TLS maturation state.

Abstract: Provided herein are pharmaceutical compositions (e.g., oral dosage formulations) comprising (S)-4-(4-(4-(((2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)oxy)methyl)benzyl)piperazin-1-yl)-3-fluorobenzonitrile, or an enantiomer, mixture of enantiomers, tautomer, isotopolog, or pharmaceutically acceptable salt thereof, and a carrier or diluent. Also provided herein are methods of preparing and methods of using the pharmaceutical compositions.

Abstract: Provided are methods of clinical treatment of chromic lymphoblastic leukemia (CLL) inhuman subjects using a bispecific-antibody which binds to CD3 and CD20.

Abstract: A device is provided that is intended to simultaneously measure and identify at least two characteristics of multiphase fluid flows through the device and/or equipment attached to the device. The device has a lower measurement medium invasive compartment comprised of at least two ultrasonic and/or acoustic transducers, a piezo-resistive sensing element, and a resistance temperature detector (RTD) to make simultaneous independent measurement. A pathway connects to the device's upper electronic compartment comprised of an accelerometer array, multiple stacked circuit boards providing power, sensing interface, processing, calculation, and network communication functionalities. The device is capable of measuring, processing, and calculating simultaneous independent pressure, temperature, flow rate, and vibration measurement. The device reports data to an external system via either wired and/or wireless communication channel.

Abstract: Described herein are methods and computer systems for classification of CD8 T-cell topology using artificial intelligence and machine learning. A plurality of histology images of tissue samples in a plurality of patients are received by a computer system. An image analysis of the plurality of histology images is performed to obtain a CD8+ T-cell abundance in the tumor parenchyma and stroma in each of the plurality of histology images. A machine learning algorithm is then trained using results of the image analysis and the CD8+ T-cell abundance in the tumor parenchyma and stroma. Based on the training, a machine learning feature space comprising a plurality of classifications is generated, and boundaries between the plurality of classifications in the machine learning feature space are identified.

Abstract: The present disclosure provides methods of identifying a subject suitable for an anti-PD-?PD-L1 antagonist therapy comprising measuring assay CD8 localization and PD-L1 expression in a tumor sample obtained from the subject. In some aspects, method further comprises administering (i) an anti-PD-?PD-L1 antagonist therapy or (ii) an anti-PD-?PD-L1 antagonist and anti-CT-LA-4 antagonist combination therapy to a subject identified as having a tumor exhibiting an excluded CD8 localization phenotype, wherein the tumor is PD-L1 negative.

Abstract: Systems, methods, and apparatus for satellite operations with a secure enclave for secure hosted payload operations are disclosed. In one or more embodiments, a disclosed method for payload operations comprises receiving, by a command receiver on a vehicle (e.g., a satellite), host commands from a host spacecraft operations center (SOC). The method further comprises reconfiguring a host payload on the vehicle according to the host commands. Also the method comprises transmitting, by a telemetry transmitter on the vehicle, host payload telemetry to the host SOC. In addition, the method comprises receiving, by a payload antenna on the vehicle, hosted commands from a secure enclave of the host SOC. Additionally, the method comprises reconfiguring a hosted payload on the vehicle according to the hosted commands. Further, the method comprises transmitting, by the payload antenna, host payload data, hosted payload data, and hosted telemetry to the secure enclave of the host SOC.

Abstract: Disclosed subject matter includes a testing method that accesses tracing data, comprising a plurality of tracing records corresponding to a plurality of test cases executed on a target system. The target system includes a plurality of microservices configured in accordance with a REST API. A dynamic test analyzer accesses the tracing data and imports a definition of the REST API. The analyzer checks each tracing record for consistency with the API definition. Based on this checking of the tracing records, one or more coverage metrics may be calculated. If any one or more of the coverage metrics fails to exceed a threshold coverage, a testing action may be required. In some cases, the testing action may be selected from either seeking additional coverage of the APIs included in the target system or seeking additional test result coverage.

Abstract: The present application is directed a computer-implemented technique for enhancing security and preventing cyber-attacks on a network. The technique includes receiving information from user equipment, selecting a first VPN server from a VPN service provider based upon a traffic-type of the user equipment, creating a policy to prevent cyber-attacks such that traffic associated with the received information of the user equipment is routed to the first VPN server, provisioning the first VPN server to last a predetermined amount of time based on the policy, coordinating the policy with a router on the network, with the traffic being sent to the VPN server via the router, and sending, after a predetermined condition is met, a request to the VPN service provider to transmit a second VPN server, and where the first VPN server terminates.

Abstract: Systems, methods, and apparatus for satellite operations with a secure enclave for secure hosted payload operations are disclosed. In one or more embodiments, a disclosed method for payload operations comprises receiving, by a command receiver on a vehicle (e.g., a satellite), host commands from a host spacecraft operations center (SOC). The method further comprises reconfiguring a host payload on the vehicle according to the host commands. Also the method comprises transmitting, by a telemetry transmitter on the vehicle, host payload telemetry to the host SOC. In addition, the method comprises receiving, by a payload antenna on the vehicle, hosted commands from a secure enclave of the host SOC. Additionally, the method comprises reconfiguring a hosted payload on the vehicle according to the hosted commands. Further, the method comprises transmitting, by the payload antenna, host payload data, hosted payload data, and hosted telemetry to the secure enclave of the host SOC.

Abstract: A method of processing a substrate according to a PECVD process is described. Temperature profile of the substrate is adjusted to change deposition rate profile across the substrate. Plasma density profile is adjusted to change deposition rate profile across the substrate. Chamber surfaces exposed to the plasma are heated to improve plasma density uniformity and reduce formation of low quality deposits on chamber surfaces. In situ metrology may be used to monitor progress of a deposition process and trigger control actions involving substrate temperature profile, plasma density profile, pressure, temperature, and flow of reactants.

Abstract: Embodiments of the present invention provide a device and a method for treating at least one of hypertension, pulmonary arteries, diabetes, obesity, heart failure, end-stage renal disease, digestive disease, urological disease, cancers, tumors, pain, asthma or chronic obstructive pulmonary disease by delivering an effective amount of a formulation to a tissue. In embodiments of the present invention, the formulation may include at least one of a gas, a vapor, a liquid, a solution, an emulsion, or a suspensions of one or more ingredients. In embodiments of the present invention, amounts of the formulation and/or energy are effective to injure or damage tissue, nerves, and nerve endings in order to relieve disease symptoms.

Abstract: A method of processing a substrate according to a PECVD process is described. Temperature profile of the substrate is adjusted to change deposition rate profile across the substrate. Plasma density profile is adjusted to change deposition rate profile across the substrate. Chamber surfaces exposed to the plasma are heated to improve plasma density uniformity and reduce formation of low quality deposits on chamber surfaces. In situ metrology may be used to monitor progress of a deposition process and trigger control actions involving substrate temperature profile, plasma density profile, pressure, temperature, and flow of reactants.

Abstract: Electronic message text classification framework selection is described. An incoming electronic message is classified using a current text classification framework. A classification of the electronic message by the current text classification framework is scored. A cost of re-training the current text classification is compared against a cost of switching to a different text classification framework. One of multiple text classification frameworks, which includes the current text classification framework and other text classification frameworks, is selected based on the score of the classification by the current text classification framework and a result of the comparison.

Abstract: Systems, methods, and computer-readable media for analyzing health information to generate medical assessments are described. Health information may include any type of information associated with the health or physical characteristics of a patient. Non-limiting examples of medical assessments include medical diagnoses, medical orders, and risk assessments. A health information analysis system may access health information associated with a patient and provide a medical assessment based on various analyses of the health information. The health information analysis system may receive feedback associated with the medical assessments, such as historical information and/or user input regarding the accuracy or completeness of the medical assessment. The feedback may be used by the health information analysis system to update the analyses of the health information.

Abstract: Systems, methods, and apparatus for satellite operations with a secure enclave for secure hosted payload operations are disclosed. In one or more embodiments, a disclosed method for payload operations comprises receiving, by a command receiver on a vehicle (e.g., a satellite), host commands from a host spacecraft operations center (SOC). The method further comprises reconfiguring a host payload on the vehicle according to the host commands. Also the method comprises transmitting, by a telemetry transmitter on the vehicle, host payload telemetry to the host SOC. In addition, the method comprises receiving, by a payload antenna on the vehicle, hosted commands from a secure enclave of the host SOC. Additionally, the method comprises reconfiguring a hosted payload on the vehicle according to the hosted commands. Further, the method comprises transmitting, by the payload antenna, host payload data, hosted payload data, and hosted telemetry to the secure enclave of the host SOC.

Abstract: Systems, methods, and apparatus for satellite operations with secure enclave for secure inband hosted payload operations are disclosed. In one or more embodiments, a method for payload operations comprises receiving, by a vehicle, host commands from a satellite operation portion of a host spacecraft operations center (SOC). The method further comprises reconfiguring a payload and at least bus component on the vehicle according to the host commands. Also, the method comprises transmitting, by the vehicle, host telemetry to the satellite operation portion of the host SOC. In addition, the method comprises receiving, by the vehicle, hosted commands from a secure enclave of the host SOC. Additionally, the method comprises reconfiguring the payload on the vehicle according to the hosted commands. Further, the method comprises transmitting, by a payload antenna on the vehicle, host payload data, hosted payload data, and the encrypted hosted telemetry to the secure enclave.

Abstract: The present application is directed to a computer readable medium. The computer readable medium contains program instructions for managing traffic in a network that, when executed by a processor, causes the processor to determine, from among plural users, information of a user in the network. The program instructions also include reviewing selection criteria of plural, dynamic, virtual private network (VPN) cloud servers in the network. The program instructions also include ranking efficiency of the plural, dynamic, virtual VPN cloud servers in the network. The program instructions further include controlling content displayed on a graphical user interface (GUI) including a dynamically changing list of users from the plural users matched with respective VPN cloud servers. The program instructions yet even further include matching the user with one of the plural, dynamic VPN cloud servers based on the ranked efficiency and the determined information of the user.

Abstract: Embodiments of the present invention provide a device and a method for treating at least one of hypertension, pulmonary arteries, diabetes, obesity, heart failure, end-stage renal disease, digestive disease, urological disease, cancers, tumors, pain, asthma or chronic obstructive pulmonary disease by delivering an effective amount of a formulation to a tissue. In embodiments of the present invention, the formulation may include at least one of a gas, a vapor, a liquid, a solution, an emulsion, or a suspensions of one or more ingredients. In embodiments of the present invention, amounts of the formulation and/or energy are effective to injure or damage tissue, nerves, and nerve endings in order to relieve disease symptoms.