Patents by Inventor J. Craig Venter

J. Craig Venter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10626429
    Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.
    Type: Grant
    Filed: July 31, 2019
    Date of Patent: April 21, 2020
    Assignee: SGI-DNA, Inc.
    Inventors: Daniel G. Gibson, Hamilton O. Smith, Clyde A. Hutchison, Lei Young, J. Craig Venter
  • Publication number: 20190376103
    Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.
    Type: Application
    Filed: July 31, 2019
    Publication date: December 12, 2019
    Inventors: Daniel G. Gibson, Hamilton O. Smith, Clyde A. Hutchison, Lei Young, J. Craig Venter
  • Publication number: 20190241921
    Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.
    Type: Application
    Filed: April 18, 2019
    Publication date: August 8, 2019
    Inventors: Daniel G. Gibson, Hamilton O. Smith, Clyde A. Hutchison, Lei Young, J. Craig Venter
  • Publication number: 20190209626
    Abstract: A probiotic composition comprises an effective amount of a combination of bacteria, wherein the combination of bacteria comprises certain at least one bacterium A selected from the Bacteroidetes phylum and certain at least one bacterium B selected from the Firmicutes phylum.
    Type: Application
    Filed: January 7, 2019
    Publication date: July 11, 2019
    Inventors: Weizhong Li, Ericka L. Anderson, Lei Huang, William Biggs, Amalio Telenti, J. Craig Venter, Karen E. Nelson
  • Patent number: 10266865
    Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.
    Type: Grant
    Filed: March 2, 2015
    Date of Patent: April 23, 2019
    Assignee: Synthetic Genomics, Inc.
    Inventors: Daniel G. Gibson, Hamilton O. Smith, Clyde A. Hutchison, Lei Young, J. Craig Venter
  • Publication number: 20180340165
    Abstract: Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.
    Type: Application
    Filed: August 6, 2018
    Publication date: November 29, 2018
    Inventors: J. Craig Venter, Hamilton O. Smith, Clyde A. Hutchison, III, Daniel G. Gibson
  • Patent number: 10041060
    Abstract: Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells.
    Type: Grant
    Filed: December 6, 2006
    Date of Patent: August 7, 2018
    Assignee: Synthetic Genomics, Inc.
    Inventors: J. Craig Venter, Hamilton O. Smith, Clyde A. Hutchison, III, Daniel G. Gibson
  • Publication number: 20180119132
    Abstract: Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.
    Type: Application
    Filed: March 22, 2017
    Publication date: May 3, 2018
    Inventors: Clyde A. Hutchison, III, Ray-Yuan Chuang, Vladimir N. Noskov, Bogumil J. Karas, Kim S. Wise, Hamilton O. Smith, John I. Glass, Chuck Merryman, Daniel G. Gibson, J. Craig Venter, Krishna Kannan, Lin Ding
  • Publication number: 20170320061
    Abstract: The present invention provides a system for receiving biological sequence information and activating the synthesis of a biological entity. The system has a receiving unit for receiving a signal encoding biological sequence information transmitted from a transmitting unit. The transmitting unit can be present at a remote location from the receiving unit. The system also has an assembly unit connected to the receiving unit, and the assembly unit assembles the biological entity according to the biological sequence information. Thus, according to the present invention biological sequence information can be digitally transmitted to a remote location and the information converted into a biological entity, for example a protein useful as a vaccine, immediately upon being received by the receiving unit and without further human intervention after preparing the system for receipt of the information.
    Type: Application
    Filed: July 19, 2017
    Publication date: November 9, 2017
    Inventors: J. Craig Venter, Daniel Gibson, John E. Gill
  • Patent number: 9718060
    Abstract: The present invention provides a system for receiving biological sequence information and activating the synthesis of a biological entity. The system has a receiving unit for receiving a signal encoding biological sequence information transmitted from a transmitting unit. The transmitting unit can be present at a remote location from the receiving unit. The system also has an assembly unit connected to the receiving unit, and the assembly unit assembles the biological entity according to the biological sequence information. Thus, according to the present invention biological sequence information can be digitally transmitted to a remote location and the information converted into a biological entity, for example a protein useful as a vaccine, immediately upon being received by the receiving unit and without further human intervention after preparing the system for receipt of the information.
    Type: Grant
    Filed: August 16, 2013
    Date of Patent: August 1, 2017
    Assignee: Synthetic Genomics, Inc.
    Inventors: J. Craig Venter, Daniel Gibson, John E. Gill
  • Patent number: 9593329
    Abstract: A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.
    Type: Grant
    Filed: October 7, 2008
    Date of Patent: March 14, 2017
    Assignee: Synthetic Genomics, Inc.
    Inventors: Daniel G. Gibson, Lei Young, John I. Glass, Gwynedd A. Benders, J. Craig Venter, Clyde A. Hutchison, III, Hamilton O. Smith
  • Patent number: 9556413
    Abstract: The invention described below relates to an enclosed cell sorting device and methods of using the device. The device is constructed so that the entire process of cell sorting can be conducted under fully anaerobic conditions to retain viability of anaerobic cells before, during, and after cell sorting. This is accomplished by creating an anaerobic atmosphere for the high speed cell sorter and all its components and by the use of airlocks that allow the introduction of anaerobic containers into the chamber containing the sample.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: January 31, 2017
    Assignee: Synthetic Genomics, Inc.
    Inventors: J. Craig Venter, Eric J. Mathur, Gerardo Vicente Toledo, Hwai Wen Chang, Wayne A. Green
  • Patent number: 9481880
    Abstract: The presently disclosed invention relates to methods of installing a genome isolated from one species (the donor) into suitably prepared cells of a second species (the recipient). Introduction of the donor genetic material into the recipient host cell effectively converts the recipient host cell into a new cell that, as a result of the operation of the donated genetic material, is functionally classified as belonging to the genus and species of the donor genetic material.
    Type: Grant
    Filed: May 1, 2008
    Date of Patent: November 1, 2016
    Assignee: Synthetic Genomics, Inc.
    Inventors: John I. Glass, Nina Alperovich, Clyde A. Hutchison, III, Carole Lartigue, Charles E. Merryman, Sanjay Vashee, J. Craig Venter
  • Patent number: 9434974
    Abstract: A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene-expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.
    Type: Grant
    Filed: December 22, 2006
    Date of Patent: September 6, 2016
    Assignee: Synthetic Genomics, Inc.
    Inventors: John I. Glass, Lei Young, Carole Lartigue, Nacyra Assad-Garcia, Hamilton O. Smith, Clyde A. Hutchison, J. Craig Venter
  • Publication number: 20160177338
    Abstract: Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
    Type: Application
    Filed: February 29, 2016
    Publication date: June 23, 2016
    Inventors: Gwynedd A. Benders, John I. Glass, Clyde A. Hutchison, III, Carole Lartigue, Sanjay Vashee, Mikkel A. Algire, Hamilton O. Smith, Charles E. Merryman, Vladimir N. Noskov, Ray-Yuan Chuang, Daniel G. Gibson, J. Craig Venter
  • Publication number: 20160177322
    Abstract: Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
    Type: Application
    Filed: February 23, 2016
    Publication date: June 23, 2016
    Inventors: Gwynedd A. Benders, John I. Glass, Clyde A. Hutchison, III, Carole Lartigue, Sanjay Vashee, Mikkel A. Algire, Hamilton O. Smith, Charles E. Merryman, Vladimir N. Noskov, Ray-Yuan Chuang, Daniel G. Gibson, J. Craig Venter
  • Patent number: 9273310
    Abstract: Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
    Type: Grant
    Filed: March 5, 2010
    Date of Patent: March 1, 2016
    Assignee: Synthetic Genomics, Inc.
    Inventors: Gwynedd A. Benders, John I. Glass, Clyde A. Hutchison, III, Carole Lartigue, Sanjay Vashee, Mikkel A. Algire, Hamilton O. Smith, Charles E. Merryman, Vladimir N. Noskov, Ray-Yuan Chuang, Daniel G. Gibson, J. Craig Venter
  • Patent number: 9267132
    Abstract: Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: February 23, 2016
    Assignee: Synthetic Genomics, Inc.
    Inventors: Gwynedd A. Benders, John I. Glass, Clyde A. Hutchison, Carole Lartigue, Sanjay Vashee, Mikkel A. Algire, Hamilton O. Smith, Charles E. Merryman, Vladimir N. Noskov, Ray-Yuan Chuang, Daniel G. Gibson, J. Craig Venter
  • Patent number: 9266929
    Abstract: The invention provides proteins from Neisseria meningitidis, including the amino acid sequences and the corresponding nucleotide sequences. The proteins are predicted to be useful antigens for vaccines and/or diagnostics.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: February 23, 2016
    Assignee: GlaxoSmithKline Biologicals SA
    Inventors: Claire Fraser, Cesira Galeotti, Guido Grandi, Erin Hickey, Vega Masignani, Marirosa Mora, Jeremy Petersen, Mariagrazia Pizza, Rino Rappuoli, Giulio Ratti, Vincenzo Scarlato, Maria Scarselli, Herve Tettelin, J. Craig Venter
  • Publication number: 20160030545
    Abstract: The invention provides proteins from Neisseria meningitidis, including the amino acid sequences and the corresponding nucleotide sequences. The proteins are predicted to be useful antigens for vaccines and/or diagnostics.
    Type: Application
    Filed: July 21, 2015
    Publication date: February 4, 2016
    Applicant: GLAXOSMITHKLINE BIOLOGICALS SA
    Inventors: Claire FRASER, Cesira GALEOTTI, Guido GRANDI, Erin HICKEY, Vega MASIGNANI, Marirosa MORA, Jeremy PETERSEN, Mariagrazia PIZZA, Rino RAPPUOLI, Giulio RATTI, Vincenzo SCARLATO, Maria SCARSELLI, Herve TETTELIN, J. Craig VENTER