Patents by Inventor Jai-Yoon Sul

Jai-Yoon Sul has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Transcriptome in vivo analysis (TIVA) and transcriptome in situ analysis (TISA)
    Patent number: 11873312
    Abstract: Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
    Type: Grant
    Filed: May 28, 2021
    Date of Patent: January 16, 2024
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Ivan J. Dmochowski, Sean B. Yeldell, Julianne C. Griepenburg, Teresa L. Rapp, Jennifer M. Singh, Jai-Yoon Sul, Jaehee Lee
  • COMPOSITIONS AND METHODS FOR GENOMIC DNA AND GENE EXPRESSION ANALYSIS IN SINGLE CELLS
    Publication number: 20240011025
    Abstract: Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
    Type: Application
    Filed: June 15, 2023
    Publication date: January 11, 2024
    Applicants: Agilent Technologies, Inc., The Trustees of the University of Pennsylvania
    Inventors: James EBERWINE, Jae-Hee LEE, Jifen LI, Stephen FISHER, Youtao LU, Junhyong KIM, Jai-Yoon SUL, Jinchun WANG, Mimi HEALY
  • Compositions and methods for genomic DNA and gene expression analysis in single cells
    Patent number: 11739321
    Abstract: Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: August 29, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: James Eberwine, Jae-Hee Lee, Jifen Li, Stephen Fisher, Youtao Lu, Junhyong Kim, Jai-Yoon Sul, Jinchun Wang, Mimi Healy
  • Transcriptome In Vivo Analysis (TIVA) and Transcriptome In Situ Analysis (TISA)
    Publication number: 20210292352
    Abstract: Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
    Type: Application
    Filed: May 28, 2021
    Publication date: September 23, 2021
    Inventors: James Eberwine, Ivan J. Dmochowski, Sean B. Yeldell, Julianne C. Griepenburg, Teresa L. Rapp, Jennifer M. Singh, Jai-Yoon Sul, Jaehee Lee
  • Transcriptome in vivo analysis (TIVA) and transcriptome in situ analysis (TISA)
    Patent number: 11021502
    Abstract: Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: June 1, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Ivan J. Dmochowski, Sean B. Yeldell, Julianne C. Griepenburg, Teresa L. Rapp, Jennifer M. Singh, Jai-Yoon Sul, Jaehee Lee
  • HIGH THROUGHPUT MICROFLUIDIC DEVICE
    Publication number: 20210031186
    Abstract: The present invention provides microfluidic devices capable of generating a flow of fluids within a chamber. The flow of fluids mix within the chamber to form a gradient of concentrations. The chamber is capable of accepting a cell culture and is accessible to retrieve the cell culture. The microfluidic devices are compatible with standard laboratory microscopes for optical analyses of the chamber.
    Type: Application
    Filed: February 7, 2019
    Publication date: February 4, 2021
    Inventors: James Eberwine, Jai-Yoon Sul, Paulo Arratia, David Gagnon, Ram Gona
  • Transcriptome transfer produces cellular phenotype conversion
    Patent number: 10883082
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Grant
    Filed: March 31, 2014
    Date of Patent: January 5, 2021
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: James Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
  • TRANSCRIPTOME TRANSFER PRODUCES CELLULAR PHENOTYPE CONVERSION
    Publication number: 20200270633
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Application
    Filed: May 11, 2020
    Publication date: August 27, 2020
    Inventors: James Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
  • COMPOSITIONS AND METHODS FOR GENOMIC DNA AND GENE EXPRESSION ANALYSIS IN SINGLE CELLS
    Publication number: 20200216841
    Abstract: Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
    Type: Application
    Filed: January 7, 2020
    Publication date: July 9, 2020
    Applicants: Agilent Technologies, Inc., The Trustees of the University of Pennsylvania
    Inventors: James EBERWINE, Jae-Hee LEE, Jifen LI, Stephen FISHER, Youtao LU, Junhyong KIM, Jai-Yoon SUL, Jinchun WANG, Mimi HEALY
  • Methods for Transfecting Nucleic Acid Into Live Cells
    Publication number: 20200206364
    Abstract: The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.
    Type: Application
    Filed: March 10, 2020
    Publication date: July 2, 2020
    Inventors: James Eberwine, Philip G. Haydon, Jai-Yoon Sul, Hajime Takano, Chia-Wen Kitty Wu, Fanyi Zeng
  • Transcriptome transfer produces cellular phenotype conversion
    Patent number: 10647960
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Grant
    Filed: April 6, 2010
    Date of Patent: May 12, 2020
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: James Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
  • Methods for phototransfecting nucleic acids into live cells
    Patent number: 10646590
    Abstract: The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.
    Type: Grant
    Filed: December 12, 2006
    Date of Patent: May 12, 2020
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: James Eberwine, Philip G. Haydon, Jai-Yoon Sul, Hajime Takano, Chia-Wen Kitty Wu, Fanyi Zeng
  • Methods for transfecting nucleic acid into live cells
    Patent number: 10583204
    Abstract: The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
    Type: Grant
    Filed: March 21, 2012
    Date of Patent: March 10, 2020
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: James Eberwine, Philip G. Haydon, Jai-Yoon Sul, Hajime Takano, Chia-Wen Kitty Wu, Fanyi Zeng
  • Compositions, methods and kits for real-time nucleic acid analysis in live cells
    Patent number: 10093917
    Abstract: The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: October 9, 2018
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: James Eberwine, Ulo Langel, Emelia Eiriksdottir, Tiina Peritz [nee Pajunen], Jai-Yoon Sul, Philip G. Haydon, Junhyong Kim
  • Transcriptome transfer produces cellular phenotype conversion
    Patent number: 9845480
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Grant
    Filed: October 12, 2015
    Date of Patent: December 19, 2017
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: James Eberwine, Jai-Yoon Sul, Tae Kyung Kim, Vickas Patel
  • Transcriptome In Vivo Analysis (TIVA) and Transcriptome In Situ Analysis (TISA)
    Publication number: 20170253876
    Abstract: Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
    Type: Application
    Filed: August 4, 2015
    Publication date: September 7, 2017
    Inventors: James Eberwine, Ivan J. Dmochowski, Sean B. Yeldell, Julianne C. Griepenburg, Teresa L. Rapp, Jennifer M. Singh, Jai-Yoon Sul, Jaehee Lee
  • Compositions, Methods and Kits for Real-Time Nucleic Acid Analysis in Live Cells
    Publication number: 20160168558
    Abstract: The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.
    Type: Application
    Filed: December 31, 2015
    Publication date: June 16, 2016
    Inventors: James Eberwine, Ulo Langel, Emelia Eiriksdottir, Tiina Peritz [nee Pajunen], Jai-Yoon Sul, Philip G. Haydon, Junhyong Kim
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
    Publication number: 20160046958
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Application
    Filed: October 12, 2015
    Publication date: February 18, 2016
    Inventors: James Eberwine, Jai-Yoon Sul, Tae Kyung Kim, Vickas Patel
  • Methods of detecting transcription
    Patent number: 9260703
    Abstract: The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.
    Type: Grant
    Filed: May 17, 2006
    Date of Patent: February 16, 2016
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James H Eberwine, Ulo Langel, Emelia Eiriksdottir, Tina Peritz, Jai-Yoon Sul, Philip G. Haydon, Junhyong Kim
  • Transcriptome transfer produces cellular phenotype conversion
    Patent number: 9157066
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Grant
    Filed: July 19, 2011
    Date of Patent: October 13, 2015
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Jai-Yoon Sul, Tae Kyung Kim, Vickas Patel