Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

Abstract: A polysulfone-urethane copolymer is disclosed, which can be used as a membrane polymer, e.g., a matrix polymer, a pore forming agent, or both, while enhancing a membrane's blood compatibility. Methods are disclosed for forming the copolymer and incorporating the copolymer in membranes (e.g., spun hollow fibers, flat membranes) and other products.

Abstract: Systems, apparatuses, and methods are described for calibrating a laser power are described. A system may include a sample to be tested and a control sample that comprises a control analyte. A user may indicate a known concentration of the control analyte to the system (e.g., by entering a concentration value into a user interface or other process). The system may perform multiple runs at different laser powers and compare the measurements of each run against expected values for the control analyte at the known concentration. From that comparison, a calibrated laser power may be computed and that computed power level can be used by the system for the running of tests on an unknown sample.

Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.

Abstract: In a method for instantly issuing a personalized financial transaction card to a customer a bank employee receives customer information and card information from the customer at a branch location. The card information may include a card personal identification number (PIN) selected by the customer. The operator inputs the customer information and at least some the card information into a data processing terminal at the branch. The customer information and the card information are communicated from the branch across a network to a card services provider. At the card services provider, the PIN is entered into a PIN database and a reference number associated with the customer and a PIN offset is generated. The reference number and at least some of the customer data and card data may be stored in a card file associated with the customer. The reference number is used to retrieve the PIN from the PIN database. The retrieved PIN is then used to apply calculations to the card file.

Abstract: An apparatus and method of minimizing pathogen cross-contamination and enhancing infection mitigation at a workplace includes placing tags at selected locations, requiring at least some workplace personnel to carry mobile devices, and logging interactions between the mobile devices and tags as tapping events. Tags can be powered or unpowered. Tapping can include RF or Bluetooth communication, and/or indicia scanning. Tapping events can be wirelessly directed to a server by the tags and/or mobile devices. The logged tapping can be used to identify high risk locations, record user movements, monitor user compliance with assigned schedules and locations, and monitor compliance with specified sanitation schedules. During mitigation of an infection event, the logged tapping can be used to identify users at high risk of direct cross-contamination due to proximity to an infected user and/or surface-mitigated cross-contamination due to occupation of a location shortly after occupation thereof by the infected user.

Abstract: A multi-stage process for the production of an ISO8217 compliant Product Heavy Marine Fuel Oil from ISO 8217 compliant Feedstock Heavy Marine Fuel Oil involving a core process under reactive conditions in a Reaction System composed of one or more reaction vessels, wherein one or more of the reaction vessels contains one or more catalysts in the form of a structured catalyst bed and is operated under reactive distillation conditions. The Product Heavy Marine Fuel Oil has a sulfur level has a maximum sulfur content (ISO 14596 or ISO 8754) between the range of 0.05 mass % to 1.0 mass. A process plant for conducting the process for conducting the process is disclosed.

Abstract: Embodiments described herein generally relate to devices, systems and methods for measuring the dose remaining in a drug delivery device that is used for delivering a dose to a patient. In some embodiments, a dose measurement system for measuring the liquid volume in a container includes a plurality of light sources which are disposed and configured to emit electromagnetic radiation toward the container. A plurality of sensors are located in the apparatus that are optically coupleable to the plurality of light sources and are disposed and configured to detect the electromagnetic radiation emitted by at least a portion of the light sources. The apparatus also includes a processing unit configured to receive data representing the portion of the detected electromagnetic radiation from each of the plurality of sensors. The processing unit is further operable to convert the received data into a signature representative of the electromagnetic radiation detected by the plurality of sensors.

Abstract: A polysulfone-urethane copolymer is disclosed, which can be used as a membrane polymer, e.g., a matrix polymer, a pore forming agent, or both, while enhancing a membrane's blood compatibility. Methods are disclosed for forming the copolymer and incorporating the copolymer in membranes (e.g., spun hollow fibers, flat membranes) and other products.

Abstract: Embodiments described herein generally relate to devices, systems and methods for measuring a volume or number of doses remaining in a drug delivery device that is used for delivering a dose to a patient. In some embodiments, a dose measurement system for measuring the liquid volume in a container includes a light guide disposed and configured to reflect electromagnetic radiation toward the container. The dose measurement system also includes a light guide disposed and configured to emit electromagnetic radiation into the light guide. A plurality of sensors are located in the apparatus that are optically coupleable to the light guide and are disposed and configured to detect the electromagnetic radiation emitted by at least a portion of the light guide. The apparatus also includes a processing unit configured to receive data representing the portion of the detected electromagnetic radiation from each of the plurality of sensors.

Abstract: Systems, apparatuses, and methods are described for calibrating a laser power are described. A system may include a sample to be tested and a control sample that comprises a control analyte. A user may indicate a known concentration of the control analyte to the system (e.g., by entering a concentration value into a user interface or other process). The system may perform multiple runs at different laser powers and compare the measurements of each run against expected values for the control analyte at the known concentration. From that comparison, a calibrated laser power may be computed and that computed power level can be used by the system for the running of tests on an unknown sample.

Abstract: The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.

Abstract: A system and method of selecting an area of interest from a plurality of images captured by a plurality of cameras are described. The method includes receiving a request for an area of interest from a plurality of images, the area of interest containing an object of interest selected by a user, the object of interest being captured in one or more images captured by one or more of the plurality of cameras, the plurality of cameras having overlapping field of views; determining a camera within the plurality of cameras that wholly contains the area of interest; transforming the physical location of the object of interest into a pixel position such that the area of interest being essentially centered around the pixel position corresponding to the physical location of the object of interest; and extracting the area of interest.

Abstract: A system and method are provided for training a machine learning system. In an embodiment, the system generates a three-dimensional model of an environment using a video sequence that includes individual frames taken from a variety of perspectives and environmental conditions. An object in the environment is identified and labeled, in some examples, by an operator, and a three-dimensional model of the object is created. Training data for the machine learning system is created by applying the label to the individual video frames of the video sequence, or by applying a rendering of the three-dimensional model to additional images or video sequences.

Abstract: An apparatus and method of minimizing pathogen cross-contamination and enhancing infection mitigation at a workplace includes placing tags at selected locations, requiring at least some workplace personnel to carry mobile devices, and logging interactions between the mobile devices and tags as tapping events. Tags can be powered or unpowered. Tapping can include RF or Bluetooth communication, and/or indicia scanning. Tapping events can be wirelessly directed to a server by the tags and/or mobile devices. The logged tapping can be used to identify high risk locations, record user movements, monitor user compliance with assigned schedules and locations, and monitor compliance with specified sanitation schedules. During mitigation of an infection event, the logged tapping can be used to identify users at high risk of direct cross-contamination due to proximity to an infected user and/or surface-mitigated cross-contamination due to occupation of a location shortly after occupation thereof by the infected user.

Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled from the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.