Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

Abstract: A neuromorphic system for switching between a multitude of functional operations includes a controlling unit and a neuron unit. The controlling unit provides a first input and a second input and regulates a multitude of bias currents. The neuron unit receives the bias currents. An input neuron group receives the first input and the second input. An excitatory neuron group is stimulated by the input neuron group. An inhibitory neuron group is electrically connected to the excitatory neuron group, and the inhibitory neuron group and the excitatory neuron group stimulate each other. An output neuron is electrically connected to the excitatory neuron group and stimulated by the excitatory neuron group to generate an output. The bias currents control the excitatory neuron group, the inhibitory neuron group and the output neuron to be in one of a high-activity state, a middle-activity state and a low-activity state.

Abstract: The claimed invention relates to a method of processing a polynucleotide, by obtaining a sense polynucleotide strand comprising a homopolymeric region that is longer that the reading section of a nanopore; synthesizing an antisense polynucleotide strand under conditions in which a nucleotide analog is incorporated at random in a reverse complement of the homopolymer region, such that the length of the homopolymer region in the antisense polynucleotide strand is shorter than the reading section of the nanopore; and moving the antisense polynucleotide strand through the nanopore such that a proportion of the antisense polynucleotide strand interacts with the nanopore.

Abstract: In one embodiment, a method for determining a dynamic head-related transfer function for a subject includes receiving audio recordings of a sound captured by audio sensors. The sound is emitted by an in-ear speaker worn by the subject. Additionally, a reference signal captured by a microphone coupled to the in-ear speaker and one or more images captured by image sensors are received. The one or more images depict a body pose of the subject while the sound is emitted by the in-ear speaker and may be used to generate a pose representation of the body pose of the subject. A head-related transfer function for each audio sensor is determined based on the pose representation, the audio recordings of the sound, and the reference signal. The dynamic head-related transfer function is determined based on the head-related transfer function for each audio sensor.

Abstract: Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid.

Abstract: The present disclosure describes a computer-implemented method that includes: monitoring, at a gas oil separation plant (GOSP) facility that includes a high-pressure production trap (HPPT) apparatus and a Dual Frequency Desalting (DFD) device, a plurality of parameters, wherein the plurality of parameters include one or more current measurements from the DFD device, as well as gas temperature and demulsifier concentration from the HPPT; based on the one or more current measurements, determining a rate of change of the one or more current measurements from the DFD device; and in response to the rate of change as well as the gas temperature and the demulsifier concentration, adjusting a demulsifier dosage being injected at the HPPT apparatus.

Abstract: Embodiments described herein generally relate to devices, systems and methods for measuring a volume or number of doses remaining in a drug delivery device that is used for delivering a dose to a patient. In some embodiments, a dose measurement system for measuring the liquid volume in a container includes a light guide disposed and configured to reflect electromagnetic radiation toward the container. The dose measurement system also includes a light guide disposed and configured to emit electromagnetic radiation into the light guide. A plurality of sensors are located in the apparatus that are optically coupleable to the light guide and are disposed and configured to detect the electromagnetic radiation emitted by at least a portion of the light guide. The apparatus also includes a processing unit configured to receive data representing the portion of the detected electromagnetic radiation from each of the plurality of sensors.

Abstract: A multi-stage process for reducing the production of a Product Heavy Marine Fuel Oil from Distressed Fuel Oil Materials (DFOM) involving a pre-treatment process that transforms the DFOM into Feedstock HMFO which is subsequently sent to a Core Process for removing the Environmental Contaminates. The Product Heavy Marine Fuel Oil complies with ISO 8217 for residual marine fuel oils and has a sulfur level has a maximum sulfur content (ISO 14596 or ISO 8754) between the range of 0.05 mass % to 1.0 mass. A process plant for conducting the process is also disclosed.

Abstract: A method of minimizing cross-contamination of pathogens between persons within a facility includes placing tags onto shared resources such as tables, desks, chairs, coffee makers, microwave ovens, sinks, bathroom stalls, etc. A mobile device senses the tags and approximates their locations, in embodiments relative to tagged anchor points. The mobile device can include any combination of tag-sensing and locating features, including a camera, LIDAR, GPS, sonic distance measurement, and accelerometers for inertial position sensing. In embodiments, the approximated tag locations can be verified and corrected as needed by using augmented reality to project the estimated tag locations onto the actual scene. The accurately determined tag locations are then used to assign resources to persons within the facility, whereby spatial distancing between persons and time intervals between usage of the same resource are maximized. A log of the resource assignments can be used to enhance contact tracing.

Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

Abstract: A multi-stage process for reducing the environmental contaminants in an ISO8217 compliant Feedstock Heavy Marine Fuel Oil involving a core desulfurizing process and a ultrasound treatment process as either a pre-treating step or post-treating step to the core process. The Product Heavy Marine Fuel Oil complies with ISO 8217 for residual marine fuel oils and has a sulfur level has a maximum sulfur content (ISO 14596 or ISO 8754) between the range of 0.05 mass % to 1.0 mass. A process plant for conducting the process is also disclosed.

Abstract: An apparatus and method of minimizing pathogen cross-contamination and enhancing infection mitigation at a workplace includes placing tags at selected locations, requiring at least some workplace personnel to carry mobile devices, and logging interactions between the mobile devices and tags as tapping events. Tags can be powered or unpowered. Tapping can include RF or Bluetooth communication, and/or indicia scanning. Tapping events can be wirelessly directed to a server by the tags and/or mobile devices. The logged tapping can be used to identify high risk locations, record user movements, monitor user compliance with assigned schedules and locations, and monitor compliance with specified sanitation schedules.

Abstract: A system and method are provided for training a machine learning system. In an embodiment, the system generates a three-dimensional model of an environment using a video sequence that includes individual frames taken from a variety of perspectives and environmental conditions. An object in the environment is identified and labeled, in some examples, by an operator, and a three-dimensional model of the object is created. Training data for the machine learning system is created by applying the label to the individual video frames of the video sequence, or by applying a rendering of the three-dimensional model to additional images or video sequences.

Abstract: A system includes a concentration sensor, a flow sensor, and a controller. The concentration sensor is configured to measure a concentration of a contaminant in a cabin of an aircraft. The flow sensor is configured to measure a flow rate of air into the cabin. The controller is configured to determine whether a concentration measurement of the contaminant in the cabin exceeds a first concentration threshold. The controller is configured to, in response to determining that the concentration measurement does not exceed the first concentration threshold, control the flow rate of air into the cabin based on a flow rate setpoint. The controller is configured to, in response to determining that the concentration measurement exceeds the first concentration threshold, control the flow rate of air into the cabin based on a flow rate setpoint and a correction factor that is based on a flow sensor tolerance.

Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Abstract: An apparatus for measuring liquid volume in a container includes a plurality of light sources for emitting electromagnetic radiation (EMR) toward the container, a plurality of sensors optically coupleable to the plurality of light sources, each sensor of the plurality of sensors for detecting the EMR emitted by at least a portion of the plurality of light sources, a temperature sensor for measuring at least one temperature associated with a liquid in the container, and at least one processor for receiving data representative of the portion of the detected EMR from each of the plurality of sensors, comparing the at least one measured temperature to a temperature guideline to identify any temperature events associated with the received data; normalizing the received data based on any temperature events associated with the received data; and converting the normalized data into a signature representative of the EMR detected by the plurality of sensors.

Abstract: The present disclosure describes a method including: receiving input data from a gas and oil separation plant (GOSP), wherein: one or more demulsifiers is being injected into an emulsion to achieve a separation, a plurality of flow rates of water and the one or more demulsifiers are being measured inside the GOSP, the input data comprises the plurality of flow rates as well as temperatures corresponding to the plurality of flow rates, and determining, for each of the one or more demulsifiers, efficiencies of the separation based on the flow rates measured at corresponding temperatures; grouping respective efficiencies of separation according to a set of temperature ranges; and generating, for at least one temperature range, a histogram for the at least one temperature range; ranking the one or more demulsifiers according to the histogram; and providing a feedback to indicate a ranked order of the one or more demulsifiers.

Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.