Abstract: A method having the following steps: running a circulation valve comprising a reactive material into the well bore on the casing; reverse-circulating an activator material in the well bore until the activator material contacts the reactive material of the circulation valve; reconfiguring the circulation valve by contact of the activator material with the reactive material; and reverse-circulating a cement composition in the well bore until the reconfigured circulation valve decreases flow of the cement composition. A circulation valve for cementing casing in a well bore, the valve having: a valve housing connected to the casing and comprising a reactive material; a plurality of holes in the housing, wherein the plurality of holes allow fluid communication between an inner diameter of the housing and an exterior of the housing, wherein the reactive material is expandable to close the plurality of holes.

Abstract: A method having the following steps: running a circulation valve comprising a reactive material into the well bore on the casing; reverse-circulating an activator material in the well bore until the activator material contacts the reactive material of the circulation valve; reconfiguring the circulation valve by contact of the activator material with the reactive material; and reverse-circulating a cement composition in the well bore until the reconfigured circulation valve decreases flow of the cement composition. A circulation valve for cementing casing in a well bore, the valve having: a valve housing connected to the casing and comprising a reactive material; a plurality of holes in the housing, wherein the plurality of holes allow fluid communication between an inner diameter of the housing and an exterior of the housing, wherein the reactive material is expandable to close the plurality of holes.

Abstract: A method having the following steps: running a circulation valve comprising a reactive material into the well bore on the casing; reverse-circulating an activator material in the well bore until the activator material contacts the reactive material of the circulation valve; reconfiguring the circulation valve by contact of the activator material with the reactive material; and reverse-circulating a cement composition in the well bore until the reconfigured circulation valve decreases flow of the cement composition. A circulation valve for cementing casing in a well bore, the valve having: a valve housing connected to the casing and comprising a reactive material; a plurality of holes in the housing, wherein the plurality of holes allow fluid communication between an inner diameter of the housing and an exterior of the housing, wherein the reactive material is expandable to close the plurality of holes.

Abstract: Improved casing strings, and methods of using improved casing strings in subterranean cementing operations, are provided. An example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of an activator. Another example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of a set retarder. An example of a method is a method of cementing in a subterranean formation. Other examples of methods are methods of cementing in a subterranean formation comprising a well bore.

Abstract: Improved casing strings, and methods of using improved casing strings in subterranean cementing operations, are provided. An example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of an activator. Another example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of a set retarder. An example of a method is a method of cementing in a subterranean formation. Other examples of methods are methods of cementing in a subterranean formation comprising a well bore.

Abstract: Methods and systems for reverse-circulation cementing in subterranean formations are provided. An example of a method is a method of cementing casing in a subterranean well bore, comprising inserting a casing into the well bore, the casing comprising a casing shoe; equipping the casing with a well head, and a casing inner diameter pressure indicator; flowing an equilibrium fluid into the well bore; flowing a cement composition into the well bore after the equilibrium fluid; determining from the well-bore pressure indicator when the well bore pressure has reached a desired value; discontinuing the flow of cement composition into the well bore upon determining that the well bore pressure has reached a desired value; and permitting the cement composition to set in the subterranean formation. Examples of systems include systems for cementing casing in a well bore.

Abstract: Improved casing strings, and methods of using improved casing strings in subterranean cementing operations, are provided. An example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of an activator. Another example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of a set retarder. An example of a method is a method of cementing in a subterranean formation. Other examples of methods are methods of cementing in a subterranean formation comprising a well bore.

Abstract: Methods and systems for reverse-circulation cementing in subterranean formations are provided. An example of a method is a method of cementing casing in a subterranean well bore, comprising inserting a casing into the well bore, the casing comprising a casing shoe; equipping the casing with a well head, and a casing inner diameter pressure indicator; flowing an equilibrium fluid into the well bore; flowing a cement composition into the well bore after the equilibrium fluid; determining from the well-bore pressure indicator when the well bore pressure has reached a desired value; discontinuing the flow of cement composition into the well bore upon determining that the well bore pressure has reached a desired value; and permitting the cement composition to set in the subterranean formation. Examples of systems include systems for cementing casing in a well bore.

Abstract: Methods and systems for reverse-circulation cementing in subterranean formations are provided. An example of a method is a method of cementing casing in a subterranean well bore, comprising inserting a casing into the well bore, the casing comprising a casing shoe; equipping the casing with a well head, and a casing inner diameter pressure indicator; flowing an equilibrium fluid into the well bore; flowing a cement composition into the well bore after the equilibrium fluid; determining from the well-bore pressure indicator when the well bore pressure has reached a desired value; discontinuing the flow of cement composition into the well bore upon determining that the well bore pressure has reached a desired value; and permitting the cement composition to set in the subterranean formation. Examples of systems include systems for cementing casing in a well bore.

Abstract: The invention is based on the discovery that certain small molecules can bind to the ATP binding site of D-Ala-D-Ala ligase, even in the absence of the enzyme's substrate, and can cause a conformational change in the enzyme structure similar to that which occurs upon binding of ATP and substrate to the enzyme. Without wishing to be bound by any theory, it is believed that such a conformational change is required for either activation or inhibition of the enzyme. The information obtained from this discovery has enabled identification of key interactions in the active site of the enzyme, as well as the design and opimization of inhibitors.

Abstract: A method of initiating fluid circulation in a well bore with a casing inner diameter and an annulus is provided. The method includes increasing the casing inner diameter fluid pressure at the top of the well bore; flowing a cement composition into the casing inner diameter at the top of the well bore; maintaining a difference in pressure between the fluid pressure of the casing inner diameter and the fluid pressure of the annulus until enough of the cement composition has entered the casing inner diameter to drive fluid circulation by the added cement composition weight; reducing the casing inner diameter fluid pressure at the top of the well bore while flowing a further portion of cement composition into the casing inner diameter; and pumping at a relatively higher fluid pressure the cement composition from the casing inner diameter into the annulus through a lower end of the casing.

Abstract: A method for sealing an annulus defined between an outer casing and an inner casing, the method having steps as follows: fastening a lock ring to an anchor base, wherein the lock ring comprises a threaded fastener of the lock ring to the head; positioning the lock ring and anchor base about the outer casing; fastening a limit clamp to an exterior surface of the outer casing; repositioning the lock ring and anchor base so that the anchor base is in contact with the limit clamp; fastening a head to the lock ring; providing an opening for fluid communication with the annulus through the head; positioning a plug about the inner casing and in contact with the inner casing and the head; positioning a seal fitting clamp about the inner casing and in contact with the plug; positioning a bull cap about the inner casing and in contact with the seal fitting clamp; and fastening the bull cap to the head.

Abstract: The present invention relates to crystallized molecules and molecular complexes which comprise the active site binding pocket or the FKBP12/FK506 binding pocket of calcineurin or close structural homologues to either binding pocket. This invention also relates to a data storage material encoded with the corresponding structure coordinates of those crystallized molecules or molecular complexes. Such data storage material is capable of displaying such molecules and molecular complexes as a graphical three-dimensional representation on a computer screen. In addition, this invention relates to methods of using the structure coordinates of those molecules or molecular complexes to solve the structure of homologous proteins. This invention also relates to methods of using the structure coordinates to screen and design compounds that bind to calcineurin or homologues thereof.

Abstract: The present invention includes vectors and methods for high throughput co-expression.

Abstract: Methods and systems for reverse-circulation cementing in subterranean formations are provided. An example of a method is a method of cementing casing in a subterranean well bore, comprising inserting a casing into the well bore, the casing comprising a casing shoe; equipping the casing with a well head, and a casing inner diameter pressure indicator; flowing an equilibrium fluid into the well bore; flowing a cement composition into the well bore after the equilibrium fluid; determining from the well-bore pressure indicator when the well bore pressure has reached a desired value; discontinuing the flow of cement composition into the well bore upon determining that the well bore pressure has reached a desired value; and permitting the cement composition to set in the subterranean formation. Examples of systems include systems for cementing casing in a well bore.

Abstract: Improved casing strings, and methods of using improved casing strings in subterranean operations, are provided. An example of a method is a method of cementing a pipe string in a subterranean formation. Another example of a method is a method of cementing a pipe string in a well bore. An example of an apparatus is an improved pipe string comprising at least one pipe section; and a delivery system of an activator; wherein the delivery system of an activator is disposed adjacent an outer surface of the at least one pipe section.

Abstract: Improved casing strings, and methods of using improved casing strings in subterranean cementing operations, are provided. An example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of an activator. Another example of an apparatus is a pipe string comprising at least one pipe section and a delivery system of a set retarder. An example of a method is a method of cementing in a subterranean formation. Other examples of methods are methods of cementing in a subterranean formation comprising a well bore.

Abstract: A method having the following steps: running a circulation valve comprising a reactive material into the well bore on the casing; reverse-circulating an activator material in the well bore until the activator material contacts the reactive material of the circulation valve; reconfiguring the circulation valve by contact of the activator material with the reactive material; and reverse-circulating a cement composition in the well bore until the reconfigured circulation valve decreases flow of the cement composition. A circulation valve for cementing casing in a well bore, the valve having: a valve housing connected to the casing and comprising a reactive material; a plurality of holes in the housing, wherein the plurality of holes allow fluid communication between an inner diameter of the housing and an exterior of the housing, wherein the reactive material is expandable to close the plurality of holes.

Abstract: Methods of servicing a wellbore in contact with a subterranean formation include passing an ester-based fluid into the wellbore and reducing a temperature of the ester-based fluid by an amount effective to cause the ester-based fluid to set, e.g., by from about 10° F. to about 20° F. The ester-based fluid includes a water in ester-based oil emulsion, a pH controlling additive, e.g., hydrated lime, in the water-phase, and an emulsifier to stabilize the pH controlling additive. Cooling the ester-based fluid causes the emulsion to destabilize, resulting in the release of the pH controlling additive. This release lowers the pH of the ester-based fluid by an effective amount to cause it to set. Wellbore servicing fluids comprising the foregoing ester-based fluid may be employed as environmentally friendly sealants or spotting fluids in wellbores.

Abstract: A method for cementing a casing in an open well bore having no surface casing, wherein an annulus is defined between the casing and the well bore, the method having: sealing the annulus at the mouth of the well bore with a seal; pumping a cement composition into the annulus through the seal; and taking circulation fluid returns from the inner diameter of the casing. A system for cementing a casing in an open well bore having no surface casing, wherein an annulus is defined between the casing and the well bore, the system having: a seal of the annulus at the mouth of the well bore; a cement composition pump fluidly connected to the annulus through the seal; and a coupling connected to the exposed end of the casing for taking circulation fluid returns from the inner diameter of the casing.