Abstract: A method for sorting a particle of interest from a plurality of particles includes the steps of determining an absorption maxima of the particle of interest, providing a light source for generating a beam of coherent light at a wavelength correlating to the absorption maxima, providing a plurality of particles on a support surface, and imparting relative motion between the beam of coherent light and the plurality of particles so as to cause differential movement between the particle of interest and the plurality of particles. The particle of interest is then collected.

Abstract: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers.

Abstract: A resonant switching-type capacitive charging power conditioner circuit includes a trap switch assembly to prevent the energy initially delivered to the circuit by an electrical energy source, from returning to the source. Once trapped, all of the energy is transferred to a capacitive store over a number of cycles. The period for each cycle is a function of the state of charge of the capacitive store, and the period decreases for each successive cycle as the charge on the capacitive store increases to its final value. Switches are turned on and off in response to the absence of certain currents in the circuit, to match the decreasing period of each successive energy transfer cycle throughout the entire energy transfer process. This adaptive clocking prevents energy from returning to the energy source, and eliminates dead time for each cycle.

Abstract: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. In one aspect, a method is provided for interacting an optical gradient field in three dimensions with a particle by interfering two beams to generate a plurality of planar fronts, providing a plurality of particles in a medium, and moving the planar fronts relative to the particles, whereby the particles are separated at least in part based upon the dielectric constant of the particles.

Abstract: A tank liquid level or volume gauge for use with volatile materials, such as propane, stored under pressure as liquid and released as gas, includes a float with a float magnet that couples with a liquid level indicator magnet across a fluid impermeable wall to provide indication of tank liquid level or volume. In another embodiment, when a predetermined tank liquid level or volume is reached during filling, the float actuates an inlet closure valve to increase inlet flow resistance, creating a shutoff signal.

Abstract: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. The invention includes methods for separating particles in a medium where the particles having differing dielectric constants by providing a medium having a dielectric constant between the dielectric constants of the particles, subjecting the particles in the media to an optical gradient field, and separating the particles.

Abstract: Methods for electronic perturbation of fluorescence, chemilluminescence and other emissive materials provide for molecular biological analysis.

Abstract: A modulation process with a fully suppressed carrier and input preprocessor filtering to produce an encoded output; for amplitude modulation (AM) and audio speech preprocessor filtering, intelligible subjective sound is produced when the encoded signal is demodulated using the RF Hearing Effect. Suitable forms of carrier suppressed modulation include single sideband (SSB) and carrier suppressed amplitude modulation (CSAM), with both sidebands present.

Abstract: Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target.

Abstract: A method of treating a patient for ventricular tachycardia using a wearable defibrillator includes monitoring the patient for a predetermined condition via one or more electrodes on the defibrillator, sending a message to the patient in response to the predetermined condition, activating the defibrillator so that the defibrillator delivers defibrillation energy to the patient, and storing at least one of the results of the monitoring, sending and activating steps in a memory on the defibrillator. The method also includes downloading information stored in the memory of the defibrillator to a base station having an external interface, and transmitting the information downloaded from the memory of the base station to an external location via the external interface of the base station.

Abstract: A defibrillator for delivering therapeutic electrical energy to a patient's heart generally includes a power source, electrodes, a patient impedance sensor, and a controller. The electrode are capable of being applied to a patient and are operably coupled to the power source. The impedance sensor is couplable to the patient. The controller controls the power source and is communication with the patient impedance sensor. In response to the patient impedance and based thereon, the controller can configure a variety of circuit options to deliver a low current flow defibrillation waveform , which has a substantially constant tilt, to the patient.

Abstract: A method of treating a patient for ventricular tachycardia using a wearable defibrillator includes monitoring the patient for a predetermined condition via one or more electrodes on the defibrillator, sending a message to the patient in response to the predetermined condition, activating the defibrillator so that the defibrillator delivers defibrillation energy to the patient, and storing at least one of the results of the monitoring, sending and activating steps in a memory on the defibrillator. The method also includes downloading information stored in the memory of the defibrillator to a base station having an external interface, and transmitting the information downloaded from the memory of the base station to an external location via the external interface of the base station.

Abstract: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers. In one aspect, a particle may be characterized by determining its optophoretic constant or signature. For example, a diseased cell has a different optophoretic constant from a healthy cell, thereby providing information, or the basis for sorting. In the event of physical sorting, various forces may be used for separation, including fluidic forces, such as through the use of laminar flow, or optical forces, or mechanical forces, such as through adhesion.

Abstract: Apparatus and methods are provided for interacting light with particles, including but not limited to biological matter such as cells, in unique and highly useful ways. Optophoresis consists of subjecting particles to various optical forces, especially optical gradient forces, and more particularly moving optical gradient forces, so as to obtain useful results. In biology, this technology represents a practical approach to probing the inner workings of a living cell, preferably without any dyes, labels or other markers.

Abstract: A method of treating a patient for ventricular tachycardia using a wearable defibrillator includes monitoring the patient for a predetermined condition via one or more electrodes on the defibrillator, sending a message to the patient in response to the predetermined condition, activating the defibrillator so that the defibrillator delivers defibrillation energy to the patient, and storing at least one of the results of the monitoring, sending and activating steps in a memory on the defibrillator. The method also includes downloading information stored in the memory of the defibrillator to a base station having an external interface, and transmitting the information downloaded from the memory of the base station to an external location via the external interface of the base station.

Abstract: In a network and a method for evaluating medical data, a disposable biochip is provided which contains a patient sample with multiple biomolecular markers, as well as a biochip identifier which characterizes the biochip. The biochip is inserted into a point of care test device, which reads the biochip identifier. The point of care test device transmits the data characterizing the biochip to a remote server having access to a data bank in which a large number of measurement protocols are stored, and which selects one of the measurement protocols for testing the sample based on the transmitted data characterizing the biochip. The measurement protocol is transmitted back to the point of care test device via the data link, where the sample is tested using the measurement protocol. The test results are evaluated in an expert system, and a diagnostic result is displayed at the point of care test device.

Abstract: The invention is in the field of using a computer to select corporate stocks for investment. A user is connected to the Internet. The user connects to the portfolio management program (PMP) host computer through the Internet. The user reviews certain strategies and their historic performance. The information is transmitted across the Internet to the user. The information transmitted includes such information as historic performance, sample holdings, modeling how the particular strategy and its holding has performed in the past. The user, after making appropriate reviews, makes a decision to purchase the names of the stocks in that portfolio. The owner of the PMP host computer collects a payment for this service. Now the user sees a list of stocks provided by the PMP host computer. If the user can then make the decision whether to accept or reject any individual stock in the generated list.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: A modulation process with a fully suppressed carrier and input preprocessor filtering to produce an encoded output; for amplitude modulation (AM) and audio speech preprocessor filtering, intelligible subjective sound is produced when the encoded signal is demodulated using the RF Hearing Effect. Suitable forms of carrier suppressed modulation include single sideband (SSB) and carrier suppressed amplitude modulation (CSAM), with both sidebands present.