Abstract: A projectile designed to be lead-free and have a ballistic coefficient ranging from about 0.13 to about 0.80 or greater for enhanced energy/performance at extended ranges may have an elongated body formed with a jacket including a wall having an end defining an ogive portion and a cavity or recess defined within the jacket and in which a core is received. The projectile can be configured in various calibers and sizes. The projectile core may be formed from a plurality of core sections, and at least one of the plurality of core sections may include tungsten powder and a lead-free binder material pressed together to form a substantially cylindrical shape or compact. One or more of the core sections further can be sintered, and the one or more core sections may be received in an end-to-end relationship within the cavity defined by the jacket to form a stacked, sectional core.

Abstract: An expandable intervertebral implant includes a superior plate having a proximal end, an opposing distal end, and a threaded first hole. An inferior plate has a proximal end, an opposing distal end, and a first bore, the inferior plate at least partially bounding an elongated channel that communicates with the first bore. A first lift screw includes a gear wheel rotatably received within the first bore of the inferior plate and a threaded stem projecting from the gear wheel and being threaded into the first hole of the superior plate. A drive screw is rotatably disposed within the channel of the inferior plate, the drive screw having a worm that engages with the gear wheel such that rotation of the drive screw facilitates rotation of the first lift screw which in turn facilitates movement of the superior plate relative to the inferior plate.

Abstract: Provided herein, in some aspects, are compositions of nucleic acids comprising an initial transcribed sequence and unique nucleic acid designs for high-yield and cost-effective production of ribonucleic acids.

Abstract: A green ceramic composition comprising (i) ceramic particles, (ii) a synthetic polymeric binder, the synthetic polymeric binder having (a) monomeric units deriving from a soft monomer, (b) monomeric units deriving from a hard non-acidic monomer, (c) monomeric units deriving from an acidic monomer, and (d) monomeric units deriving from a hydroxy-functionalized monomer, and (iii) water.

Abstract: In the various aspects and embodiments, this disclosure provides messenger RNA (mRNA) constructs for therapeutic delivery, as well as methods for making such mRNA constructs and pharmaceutical compositions comprising the same (including mRNA vaccine compositions). In still other aspects, the invention provides methods for treating patients by expression of therapeutic proteins, including for preventing or reducing probability of infection by, or illness involving, a virus. Exemplary viruses include coronaviruses (such as SARS-CoV-2 and variants therefore) and influenza viruses, among others.

Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

Abstract: A system and method for reworking a flip chip includes use of a mill to remove an old flip chip, and a pick-and-place device for putting a new flip chip in place at the same location. The process may be automated, with the removal and the placement occurring sequentially without need for operator intervention. Other devices and processes may be part of the system/machine and process, for example cleaning following the milling, fluxing prior to the placement, and heating to cause solder reflow, to secure the new flip chip in place. Underfill may be employed to make for a more mechanically robust mounting of the new flip chip.

Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

Abstract: Provided herein, are compositions based on retroviruses (e.g., lentiviruses) comprising one or more nucleic acid molecules encoding retroviral Pol polyprotein components and a nucleic acid molecule comprising one or more transgene sequences flanked by long terminal repeat sequences, for delivery of the one or more transgenes to a target cell ex vivo or in vivo. The compositions are useful for delivering to a target cell (e.g., hematopoietic stem cells (HSCs), liver cells, ocular cells, muscle cells, epithelial cells, T cells, etc.) and/or stably expressing any transgene (e.g., beta-globin, Factor VIII, RP GTPase regulator (RPGR), dystrophin, cystic fibrosis transmembrane conductance regulator (CFTR), a chimeric antigen receptor, etc.) with a biological effect to treat and/or ameliorate the symptoms associated with any disorder related to gene expression (e.g., sickle cell disease, beta-thalassemia, haemophilia B, retinitis pigmentosa, Duchenne muscular dystrophy, cystic fibrosis, cancer, etc.).

Abstract: Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.

Abstract: Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.

Abstract: Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.

Abstract: An expandable intervertebral implant includes a superior plate having a proximal end, an opposing distal end, and a threaded first hole. An inferior plate has a proximal end, an opposing distal end, and a first bore, the inferior plate at least partially bounding an elongated channel that communicates with the first bore. A first lift screw includes a gear wheel rotatably received within the first bore of the inferior plate and a threaded stem projecting from the gear wheel and being threaded into the first hole of the superior plate. A drive screw is rotatably disposed within the channel of the inferior plate, the drive screw having a worm that engages with the gear wheel such that rotation of the drive screw facilitates rotation of the first lift screw which in turn facilitates movement of the superior plate relative to the inferior plate.

Abstract: Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.

Abstract: Two shot injection molding processes are disclosed for making thermoplastic parts of two different compositions, the second shot composition having a higher thermal conductivity than the first, and the mold cavity surface temperature is greater than 70° C., preferably 70-100° C. In embodiments, the cavity surface temperature is within 20° C. of the Vicat temperature for the composition being injected, the cavity pressure is between 20 and 150 MPa, and the melt temperature of the second shot material is 200-400° C., or 250-340° C. The resulting molded part provides for improved thermal conductivity between the two molded compositions.

Abstract: A thermoforming trim press has one or more connecting arms for driving a movable platen, each arm having a pivotal connection between two adjacent sections of each connecting arm which is normally locked during normal press operation whereby the movable platen is able to move further back from a normal retracted position to increase the available clearance when changing or maintaining tools mounted on the movable platen. The pivoting ability of the connecting arm sections also simplifies adjusting the stroke of the press. A counterweight may be driven by the crank pins to match the press stroke adjustments and to thereby correspond the movement of the counterweight to the movement of the movable platen.

Abstract: A system and method for reworking a flip chip includes use of a mill to remove an old flip chip, and a pick-and-place device for putting a new flip chip in place at the same location. The process may be automated, with the removal and the placement occurring sequentially without need for operator intervention. Other devices and processes may be part of the system/machine and process, for example cleaning following the milling, fluxing prior to the placement, and heating to cause solder reflow, to secure the new flip chip in place. Underfill may be employed to make for a more mechanically robust mounting of the new flip chip.

Abstract: Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.

Abstract: A suturer may include a base mechanism. The base mechanism may include a first arm having a first driving member. The base mechanism may include a second arm having a second driving member. The suturer may be configured to selectively pass a needle from an enclosed position within the first arm to an enclosed position within the second arm.

Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.