Abstract: Systems and related methods for preparing and treating damage in an ankle joint. The system may include one or more of a reamer, a surgical device, a tibial implant, a placement tool, and a talar implant. The reamer may prepare an intramedullary canal for implantation of an implant. The reamer may include a plurality of nested reamer elements that may be expanded into the intramedullary canal. The surgical device may include a housing containing the reamer and be configured to orient the reamer with the intramedullary canal. The tibial implant may include a plurality of stem components. The placement tool may be configured to combine the plurality of stem components. The talar implant may be configured to be implanted in a talus.

Abstract: An expandable intervertebral implant includes a superior plate having a proximal end, an opposing distal end, and a threaded first hole. An inferior plate has a proximal end, an opposing distal end, and a first bore, the inferior plate at least partially bounding an elongated channel that communicates with the first bore. A first lift screw includes a gear wheel rotatably received within the first bore of the inferior plate and a threaded stem projecting from the gear wheel and being threaded into the first hole of the superior plate. A drive screw is rotatably disposed within the channel of the inferior plate, the drive screw having a worm that engages with the gear wheel such that rotation of the drive screw facilitates rotation of the first lift screw which in turn facilitates movement of the superior plate relative to the inferior plate.

Abstract: Provided herein, in some aspects, are compositions of nucleic acids comprising an initial transcribed sequence and unique nucleic acid designs for high-yield and cost-effective production of ribonucleic acids.

Abstract: In the various aspects and embodiments, this disclosure provides messenger RNA (mRNA) constructs for therapeutic delivery, as well as methods for making such mRNA constructs and pharmaceutical compositions comprising the same (including mRNA vaccine compositions). In still other aspects, the invention provides methods for treating patients by expression of therapeutic proteins, including for preventing or reducing probability of infection by, or illness involving, a virus. Exemplary viruses include coronaviruses (such as SARS-CoV-2 and variants therefore) and influenza viruses, among others.

Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

Abstract: Provided herein, are compositions based on retroviruses (e.g., lentiviruses) comprising one or more nucleic acid molecules encoding retroviral Pol polyprotein components and a nucleic acid molecule comprising one or more transgene sequences flanked by long terminal repeat sequences, for delivery of the one or more transgenes to a target cell ex vivo or in vivo. The compositions are useful for delivering to a target cell (e.g., hematopoietic stem cells (HSCs), liver cells, ocular cells, muscle cells, epithelial cells, T cells, etc.) and/or stably expressing any transgene (e.g., beta-globin, Factor VIII, RP GTPase regulator (RPGR), dystrophin, cystic fibrosis transmembrane conductance regulator (CFTR), a chimeric antigen receptor, etc.) with a biological effect to treat and/or ameliorate the symptoms associated with any disorder related to gene expression (e.g., sickle cell disease, beta-thalassemia, haemophilia B, retinitis pigmentosa, Duchenne muscular dystrophy, cystic fibrosis, cancer, etc.).

Abstract: Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.

Abstract: Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.

Abstract: Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.

Abstract: An expandable intervertebral implant includes a superior plate having a proximal end, an opposing distal end, and a threaded first hole. An inferior plate has a proximal end, an opposing distal end, and a first bore, the inferior plate at least partially bounding an elongated channel that communicates with the first bore. A first lift screw includes a gear wheel rotatably received within the first bore of the inferior plate and a threaded stem projecting from the gear wheel and being threaded into the first hole of the superior plate. A drive screw is rotatably disposed within the channel of the inferior plate, the drive screw having a worm that engages with the gear wheel such that rotation of the drive screw facilitates rotation of the first lift screw which in turn facilitates movement of the superior plate relative to the inferior plate.

Abstract: Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.

Abstract: A thermoforming trim press has one or more connecting arms for driving a movable platen, each arm having a pivotal connection between two adjacent sections of each connecting arm which is normally locked during normal press operation whereby the movable platen is able to move further back from a normal retracted position to increase the available clearance when changing or maintaining tools mounted on the movable platen. The pivoting ability of the connecting arm sections also simplifies adjusting the stroke of the press. A counterweight may be driven by the crank pins to match the press stroke adjustments and to thereby correspond the movement of the counterweight to the movement of the movable platen.

Abstract: Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.

Abstract: A suturer may include a base mechanism. The base mechanism may include a first arm having a first driving member. The base mechanism may include a second arm having a second driving member. The suturer may be configured to selectively pass a needle from an enclosed position within the first arm to an enclosed position within the second arm.

Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

Abstract: Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.

Abstract: A thermoforming trim press has one or more connecting arms for a movable platen which each have a pivotal connection between the adjacent ends of two adjacent sections into which each connecting arm is divided. The pivotal connection is normally locked to keep the sections in end to end alignment during normal press operation, but can be released to allow pivoting between the sections to be able to draw the movable platen further back from the normal retracted position to increase the available clearance when changing or maintaining tools mounted on the movable platen. The pivoting ability of the connecting arm sections also simplifies adjusting the stroke of the press while this is normally done by relocating a crank pin on flywheels driving the connecting arms. The change of position does not affect the movable platen when the arm sections may pivot with respect to each other allowing relocating the crank pins to adjust the stroke without requiring disassembling of the crank pins.

Abstract: A customer satisfaction prediction tool is usable to determine a point value for each of a plurality of leading and lagging service indicators associated with a plurality of service cases. The leading indicators may be based on currently open service cases and the lagging indicators may be based on closed service cases. The tool also is usable to add together the point values to produce a total point value, compute an index score based on the total point value, and display the computed index scores.

Abstract: A suturer may include a base mechanism. The base mechanism may include a first arm and a second arm. The suturer may be configured to selectively pass a needle from an enclosed position within the first arm to an enclosed position within the second arm.