Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

Abstract: A portable audio system includes a housing defining a common acoustic cavity a woofer disposed at a first end of the housing in the common acoustic cavity and a tweeter disposed at a second end of the housing, opposite to the first end, in the common acoustic cavity facing the woofer. A waveguide is disposed in the common acoustic cavity between the woofer and the tweeter to separate the woofer and the tweeter. The waveguide disperses and distributes sound waves generated by the woofer and tweeter.

Abstract: Methods and compositions of erythroid cells that produce adult ?-hemoglobin, generated by culturing CD31+, CD31+/CD34+ or CD34+ cells from embryonic stem cells under serum-free culture conditions.

Abstract: An input from a current user selects, for display, a profile for another user. The current user's profile, and the selected profile are compared to identify a difference. A replication tag, corresponding to the difference, is displayed in the display for the selected profile. The information giving rise to the identified difference is replicated from the selected profile to the current user's profile, based on user actuation of the replication tag.

Abstract: A method and apparatus are provided for determining the layout of a form automatically to accommodate text in the form. Under the method and apparatus, a set of parameters define the sizing and alignment of elements of the form. Sizing and positioning instructions that are separate from the sizing and alignment parameters set the sizing and positioning of elements of the form based on the sizing and alignment parameters. The instructions determine the minimum size for the form, based in part on text in the form. The difference between the minimum size for the form and a desired size for the form is then distributed across the form to size and position the elements in the form.

Abstract: Medical devices having restrictive access, and methods thereof are provided.

Abstract: Systems and methods are provided methods for providing an online interface for displaying hierarchically or logically arranged user-generated content. One method including receiving, from a first user over a network, topical user-generated electronic content, the topical user-generated electronic content being related to a topic of a web page; displaying the topical user-generated electronic content received from the first user on the web page; receiving, from a second user over the network, responsive user-generated electronic content and an indication of a desire to post the responsive user-generated electronic content on the web page in association with the topical user-generated electronic content; and displaying, adjacent to the topical user-generated electronic content on the web page, a user element or link that a third user can manipulate to display the responsive user-generated electronic content adjacent to the topical user-generated electronic content.

Abstract: This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.

Abstract: Systems and methods are provided methods for providing an online interface for displaying hierarchically or logically arranged user-generated content. One method including receiving, from a first user over a network, topical user-generated electronic content, the topical user-generated electronic content being related to a topic of a web page; displaying the topical user-generated electronic content received from the first user on the web page; receiving, from a second user over the network, responsive user-generated electronic content and an indication of a desire to post the responsive user-generated electronic content on the web page in association with the topical user-generated electronic content; and displaying, adjacent to the topical user-generated electronic content on the web page, a user element or link that a third user can manipulate to display the responsive user-generated electronic content adjacent to the topical user-generated electronic content.

Abstract: The present invention relates generally to isolated to arginine deiminase (ADI) proteins that have reduced cross-reactivity with anti-ADI-PEG 20 antibodies as compared to ADI-PEG 20, but which can have functional characteristics comparable to or better than ADI-PEG 20, compositions comprising the ADI proteins, and related methods of treating arginine-dependent diseases or related diseases such as cancer.

Abstract: Concepts and technologies are described herein for collecting and displaying information. A client device executes one or more application programs for providing operations with respect to entity-related data that relates to one or more entities. The application programs analyze the entity-related data to identify one or more entities associated with the entity-related data and transmit entity information relating to the entities to a server or database. Information relating to the entity is retrieved from the server or database and is presented to a user of the client device.

Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented. Also provided herein are defined culture systems for maintaining human pluripotent stem cells in a substantially undifferentiated state, where the defined culture system comprises human pluripotent stem cells, a defined culture medium, and at least one polypeptide selected from the group consisting of residues 43 to 378 of SEQ ID NO:1 and residues 45 to 378 of SEQ ID NO:1, and where the defined culture medium comprises insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEM/F12, NaHCO3, and one of TGF? and NODAL.

Abstract: Disclosed herein is the generation of a permissive murine strain (NSGW) that exhibits enhanced levels of human chimerism without pre-conditioning.

Abstract: An input from a current user selects, for display, a profile for another user. The current user's profile, and the selected profile are compared to identify a difference. A replication tag, corresponding to the difference, is displayed in the display for the selected profile. The information giving rise to the identified difference is replicated from the selected profile to the current user's profile, based on user actuation of the replication tag.

Abstract: Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described.

Abstract: This invention relates to hematopoietic precursors derived from human embryonic stem cells. In the culture of differentiated cells from human ES cells, the fully committed hematopoietic precursors are CD34+ and CD43+ but not CD45+. If the cells are cultured until they express CD45, then the cells lose the ability to produce differentiated cells of the lymphoid lineages.

Abstract: Provided are chimeric arginine deiminases, including pegylated chimeric arginine deiminases, and related compositions and methods of use thereof, including methods of treating cancer.

Abstract: A customer relationship management (CRM) system utilizes a form definition, user interface (UI) definitions, and UI code to generate Web pages for interacting with CRM data. A CRM plug-in is configured to execute in conjunction with a personal information manager (PIM) client application and to utilize the same form definition, UI definitions, and UI code utilized by the CRM system to generate the Web-based CRM interface. The UI definitions are utilized to present UI controls for performing CRM-related functionality in the context of a UI generated by the PIM client application. The UI code utilized by the CRM system to implement Web-based UI controls can be utilized to implement the UI controls in the context of the PIM client application. The form definition utilized by the CRM system might be utilized to display CRM fields in the UI presented by the PIM client application.

Abstract: A method for producing mature hepatocytes having functional hepatic enzyme activity from human pluripotent cells is disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122, the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-let-7c, a microRNA having the seed sequence of the microRNA miR-122, a microRNA having the seed sequence of the microRNA miR-let-7c, or a combination thereof into one or more fetal hepatocytes. The resulting cells differentiate into mature hepatocytes that exhibit functional hepatic enzyme activity, and can be used in drug metabolism and toxicity testing, in the study of viruses that target hepatic tissue, and as therapeutics. A related method of maintaining the functional hepatic enzyme activity of primary hepatocytes over time is also disclosed.

Abstract: Methods for the determination of tissue-specific genetic variation are provided. For example, in certain aspects methods for using iPS cell-derived specific cell types for differential molecular analysis of tissue-specific genetic variation are described.