Abstract: Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Abstract: The methods disclosed herein provide for clonal plant production using a morphogenic gene to produce non-transgenic and transgenic plants wherein the morphogenic gene does not integrate into the genome of the plant.

Abstract: The present disclosure provides methods of generating doubled haploid plants using a polynucleotide sequence encoding the truncated ZM-ODP2 polypeptide for parthenogenic haploid induction and haploid chromosome doubling.

Abstract: Heat exchangers (also referred to as exchangers herein) are provided that fit within a bottom sump of a distillation column. These heat exchangers may be at least partially submerged in the bottoms fluid of the distillation column so that the exterior surface of the heat exchanger can contribute to the total area of the heat exchanger. The internal configuration of the exchanger allows for annular coaxial flow of the hot fluid (condensing vapor stream) and eliminates the need for top and bottom channel heads.

Abstract: A plunger for use with a syringe has a plunger body with a proximal end, a distal end, and a circumferential sidewall extending between the proximal end and the distal end along a plunger longitudinal axis. The plunger further has at least one resiliently deflectable retaining member having a first segment attached to the plunger body and a second segment protruding toward the distal end of the plunger body and deflectable relative to the first segment. The plunger further has at least one actuation member associated with the at least one resiliently deflectable retaining member. The at least one actuation member interacts with a piston to deflect the at least one resiliently deflectable retaining member upon rotation of the plunger relative to the piston. The plunger is engageable with the piston regardless of a rotational orientation of the piston relative to the plunger.

Abstract: The present disclosure relates to methods and products for use in detecting target RNA sequences in a sample, based on a process which nicks the DNA strand of a DNA/RNA duplex and amplifies a product thereof

Abstract: Multistranded conductors adapted to in vivo environments and methods of making same are disclosed, wherein specific configurations, sequences and directions of wire wraps provide implantable multistranded coils with increased cycle life in in vivo environments as well as desirable electrical performance characteristics.

Abstract: The present invention relates to a genetic probe comprising: a nanoparticle; an oligonucleotide probe anchored to the surface of the nanoparticle, comprising an oligonucleotide backbone with a tag incorporated therein via a linker group; and a reference probe anchored to the surface of the nanoparticle, wherein the reference probe comprises a marker; wherein either (a) the tag is an organic fluorescent tag and the marker is a transition metal-based fluorescent marker; or (b) the tag is a redox-active tag and the marker is a transition metal-based redox-active marker. The invention also relates to a composition or kit containing a probe of the invention.

Abstract: A method of separating excess resin from at least one object includes: (a) stereolithographically producing at least one object, each object having at least one retention feature (32) formed thereon, each object carrying excess resin on a surface thereof; then (b) mounting each object on at least one transfer frame (21), each transfer frame having at least one retention member (22) that mates with the retention feature; (c) connecting each transfer frame to a rotor with the at least one object carried thereon; (d) centrifugally separating excess resin from each object by spinning the rotor with each transfer frame connected thereto while the at least one object remains connected to each transfer frame by the retention feature; then (e) removing each transfer frame from the rotor, with excess resin separated from each at least one object thereon.

Abstract: A spray dispenser is provided, the spray dispenser comprising a container for holding a liquid to be dispensed and a dispensing assembly for extracting liquid from said container, the dispensing assembly comprising a pressure-relief valve for release of excessive pressure in the container and a filter arranged to permit egress of gas through the filter, inhibit egress of liquid from the container and to inhibit ingress of contaminants into the container through the pressure relief valve. A dispensing assembly for use is a spray dispenser is also provided.

Abstract: An example of an apparatus is provided. The apparatus includes an input device to receive design data. The design data includes information about a geometry and load characteristics of an object. The apparatus further includes a structural design engine to generate print data to print the object on a three-dimensional printer based on the design data. The apparatus also includes a fluid design engine to generate fluidic data. The fluidic data represents a fluid channel within the object. In addition, the apparatus includes an output engine to generate an output file based on the print data and the fluidic data.

Abstract: A system for creating surrogate models is provided. The system includes a user computing device including a user interface, a database, and a processor. The processor is configured to receive, from the user computing device, a workflow request, determine at least one application in the database required to complete the workflow request, retrieve, from the database, the at least one application and meta-data associated with the at least one application, and determine that a user has proper tenancies to use the at least one application. The processor is further configured to execute, using the at least one application on an external computing device, a workflow associated with the workflow request based at least in part on the meta-data, generate, based on execution of the workflow request, new meta-data associated with the at least one application, and transmit the new meta-data to the database.

Abstract: A spray dispenser is provided, the spray dispenser comprising a container for holding a liquid to be dispensed and a dispensing assembly for extracting liquid from said container, the dispensing assembly comprising a pressure-relief valve for release of excessive pressure in the container and a filter arranged to permit egress of gas through the filter, inhibit egress of liquid from the container and to inhibit ingress of contaminants into the container through the pressure relief valve. A dispensing assembly for use is a spray dispenser is also provided.

Abstract: An example device includes: a memory storing instructions; and a processor connected to the memory. The instructions are to cause the processor to: receive predetermined locations of a fluidic input location and fluidic output locations at a three-dimensional (3D) object model; generate respective paths between the fluidic input and each of the fluidic outputs via associated portions of the 3D object model; replace the respective paths with respective hollow connectors that have respective fluidic resistance selected such that each of the fluidic outputs have a predetermined flow rate from the fluidic input to the fluid outputs; and store, at the memory, data indicative of locations and dimensions of the respective hollow connectors, relative to the fluidic input and the fluidic outputs, the data for use by a three-dimensional printer to print a part that includes the fluidic input, the fluidic outputs and the respective hollow connectors.

Abstract: Described embodiments generally relate to a method for improving data accuracy of sleep pattern data. The method comprises receiving first data relating to at least one sleep pattern metric; receiving second data relating to the at least one sleep pattern metric, wherein the second data is data entered by a user; determining the difference between the first data and the second data to calculate a data infidelity value; and in response to the data infidelity value exceeding a predetermined threshold, prompting a user to enter third data relating to at least one metric.

Abstract: Methods and systems for predicting the post-operative effective lens position (ELP) of a prosthetic intraoccular lens (IOL) in cataract surgery using novel pre-operative measurement(s) of the natural lens curvatures.

Abstract: In an example of a method for parts packing, at least one part to be printed is identified. A placement and an orientation of the at least one identified part in a build volume of a three-dimensional (3D) printer is determined that optimizes a thermal uniformity inside the build volume.

Abstract: A spray dispenser is provided, the spray dispenser comprising a container for holding a liquid to be dispensed and a dispensing assembly for extracting liquid from said container, the dispensing assembly comprising a pressure-relief valve for release of excessive pressure in the container and a filter arranged to permit egress of gas through the filter, inhibit egress of liquid from the container and to inhibit ingress of contaminants into the container through the pressure relief valve. A dispensing assembly for use is a spray dispenser is also provided.

Abstract: An example of an apparatus is provided. The apparatus includes an input device to receive design data. The design data includes information about a geometry and load characteristics of an object. The apparatus further includes a structural design engine to generate print data to print the object on a three-dimensional printer based on the design data. The apparatus also includes a fluid design engine to generate fluidic data. The fluidic data represents a fluid channel within the object.

Abstract: An example device includes: a memory storing instructions; and a processor connected to the memory. The instructions are to cause the processor to: receive predetermined locations of a fluidic input location and fluidic output locations at a three-dimensional (3D) object model; generate respective paths between the fluidic input and each of the fluidic outputs via associated portions of the 3D object model; replace the respective paths with respective hollow connectors that have respective fluidic resistance selected such that each of the fluidic outputs have a predetermined flow rate from the fluidic input to the fluid outputs; and store, at the memory, data indicative of locations and dimensions of the respective hollow connectors, relative to the fluidic input and the fluidic outputs, the data for use by a three-dimensional printer to print a part that includes the fluidic input, the fluidic outputs and the respective hollow connectors.