Patents by Inventor James Unch

James Unch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Activation of bioluminescence by structural complementation
    Patent number: 9797889
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: October 24, 2017
    Assignee: Promega Corporation
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • Coelenterazine derivatives and methods of using same
    Patent number: 9487520
    Abstract: The invention provides coelenterazine derivatives which are substrates for a non-luminescent enzyme and a pro-substrate for a luminescent enzyme. The invention also provides a method of using the derivatives.
    Type: Grant
    Filed: November 2, 2011
    Date of Patent: November 8, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch, Wenhui Zhou
  • ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION
    Publication number: 20160282340
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Application
    Filed: March 17, 2016
    Publication date: September 29, 2016
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • LUMINOGENIC AND FLUOROGENIC COMPOUNDS AND METHODS TO DETECT MOLECULES OR CONDITIONS
    Publication number: 20160176830
    Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: November 25, 2014
    Publication date: June 23, 2016
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • IMIDAZO[1,2-alpha]PYRAZINE DERIVATIVES
    Publication number: 20160002703
    Abstract: The present invention provides, among other things, imidazo[1,2-?]pyrazine derivatives. The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
    Type: Application
    Filed: September 21, 2015
    Publication date: January 7, 2016
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch
  • Imidazo[1,2-a]pyrazine derivatives
    Patent number: 9139836
    Abstract: The present invention provides, among other things, imidazo[1,2-?]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: September 22, 2015
    Assignee: PROMEGA CORPORATION
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20150064731
    Abstract: The present invention provides, among other things, imidazo[1,2-?]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
    Type: Application
    Filed: August 18, 2014
    Publication date: March 5, 2015
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch
  • Detection of hydrogen peroxide
    Patent number: 8937183
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: January 20, 2015
    Assignee: Promega Corporation
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION
    Publication number: 20140363375
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Application
    Filed: March 13, 2014
    Publication date: December 11, 2014
    Applicant: Promega Corporation
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION
    Publication number: 20140348747
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Application
    Filed: March 13, 2014
    Publication date: November 27, 2014
    Applicant: Promega Corporation
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • DETECTION OF HYDROGEN PEROXIDE
    Publication number: 20140329257
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Application
    Filed: July 21, 2014
    Publication date: November 6, 2014
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • Imidazo[1,2-?]pyrazine derivatives
    Patent number: 8809529
    Abstract: The present invention provides, among other things, imidazo[1,2-?]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
    Type: Grant
    Filed: November 2, 2011
    Date of Patent: August 19, 2014
    Assignee: Promega Corporation
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch
  • Detection of hydrogen peroxide
    Patent number: 8785652
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: July 22, 2014
    Assignee: Promega Corporation
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • LUMINOGENIC AND FLUOROGENIC COMPOUNDS AND METHODS TO DETECT MOLECULES OR CONDITIONS
    Publication number: 20140154716
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: June 10, 2013
    Publication date: June 5, 2014
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Patent number: 8476450
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Grant
    Filed: July 3, 2008
    Date of Patent: July 2, 2013
    Assignee: Promega Corporation
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • DETECTION OF HYDROGEN PEROXIDE
    Publication number: 20130045497
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Application
    Filed: August 16, 2012
    Publication date: February 21, 2013
    Applicant: Promega Corporation
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • NOVEL COELENTERAZINE SUBSTRATES AND METHODS OF USE
    Publication number: 20120117667
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and novel coelenterazine-based substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: November 2, 2011
    Publication date: May 10, 2012
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch
  • COELENTERAZINE DERIVATIVES AND METHODS OF USING SAME
    Publication number: 20120107849
    Abstract: The invention provides coelenterazine derivatives which are substrates for a non-luminescent enzyme and a pro-substrate for a luminescent enzyme. The invention also provides a method of using the derivatives.
    Type: Application
    Filed: November 2, 2011
    Publication date: May 3, 2012
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch, Wenhui Zhou
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Patent number: 7951550
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: May 31, 2011
    Assignee: Promega Corporation
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Publication number: 20080299593
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: July 3, 2008
    Publication date: December 4, 2008
    Applicant: Promega Corporation
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou