Patents by Inventor James Unch

James Unch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • NOVEL COELENTERAZINE SUBSTRATES AND METHODS OF USE
    Publication number: 20120117667
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and novel coelenterazine-based substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: November 2, 2011
    Publication date: May 10, 2012
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch
  • COELENTERAZINE DERIVATIVES AND METHODS OF USING SAME
    Publication number: 20120107849
    Abstract: The invention provides coelenterazine derivatives which are substrates for a non-luminescent enzyme and a pro-substrate for a luminescent enzyme. The invention also provides a method of using the derivatives.
    Type: Application
    Filed: November 2, 2011
    Publication date: May 3, 2012
    Inventors: Dieter H. Klaubert, Poncho Meisenheimer, James Unch, Wenhui Zhou
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Patent number: 7951550
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: May 31, 2011
    Assignee: Promega Corporation
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Publication number: 20080299593
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: July 3, 2008
    Publication date: December 4, 2008
    Applicant: Promega Corporation
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • METHODS TO QUENCH LIGHT FROM OPTICAL REACTIONS
    Publication number: 20080248511
    Abstract: The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided.
    Type: Application
    Filed: March 26, 2008
    Publication date: October 9, 2008
    Inventors: William Daily, Erika Hawkins, Dieter Klaubert, Mark McDougall, James Unch, Keith V. Wood, Wenhui Zhou, Ji Zhu
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Publication number: 20070015790
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.
    Type: Application
    Filed: May 31, 2006
    Publication date: January 18, 2007
    Inventors: James Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John Shultz, James Unch, Michael Valley, Keith Wood, Wenhui Zhou