Patents by Inventor Jeffrey A. Jeddeloh

Jeffrey A. Jeddeloh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DIRECT OLIGONUCLEOTIDE SYNTHESIS ON CELLS AND BIOMOLECULES
    Publication number: 20220186213
    Abstract: The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3?-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3? hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
    Type: Application
    Filed: March 4, 2022
    Publication date: June 16, 2022
    Applicant: DNA Script SAS
    Inventors: Xavier Godron, Adrian Horgan, Sylvain Gariel, Jeffrey Jeddeloh, Robert Nicol, Thomas Ybert
  • Direct oligonucleotide synthesis on cells and biomolecules
    Patent number: 11268091
    Abstract: The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3?-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3? hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
    Type: Grant
    Filed: December 10, 2019
    Date of Patent: March 8, 2022
    Assignee: DNA Script SAS
    Inventors: Xavier Godron, Adrian Horgan, Sylvain Gariel, Jeffrey Jeddeloh, Robert Nicol, Thomas Ybert
  • COMPOSITIONS AND METHODS FOR BISULFITE CONVERTED SEQUENCE CAPTURE
    Publication number: 20150141256
    Abstract: This invention relates generally to composition and methods for characterizing a methylome which comprises all or substantially all methylation states of a genome. In particular, a plurality of oligonucleotides, each representing nearly every possible methylation state of the cytosine position of each CG dinucleotide pair within a target nucleic acid of interest, and methods of using the plurality are provided herein.
    Type: Application
    Filed: July 23, 2014
    Publication date: May 21, 2015
    Inventors: Daniel Burgess, Jeffrey Jeddeloh
  • Methods and systems for uniform enrichment of genomic regions
    Patent number: 8383338
    Abstract: The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.
    Type: Grant
    Filed: February 23, 2009
    Date of Patent: February 26, 2013
    Assignee: Roche NimbleGen, Inc.
    Inventors: Jacob Kitzman, Todd Richmond, Mark D'Ascenzo, Thomas Albert, Matthew Rodesch, Jeffrey Jeddeloh, Christina Middle
  • Methods for quantitative determination of methylation density in a DNA locus
    Patent number: 8361719
    Abstract: The present invention is a novel method of determining the average DNA methylation density of a locus of interest within a population of DNA fragments.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: January 29, 2013
    Assignee: Orion Genomics LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey
  • GENE METHYLATION IN CANCER DIAGNOSIS
    Publication number: 20120122090
    Abstract: The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of cancer.
    Type: Application
    Filed: May 5, 2011
    Publication date: May 17, 2012
    Applicant: ORION GENOMICS LLC
    Inventors: MUHAMMAD A. BUDIMAN, JEFFREY A. JEDDELOH, REBECCA MALONEY, YULIA KORSHUNOVA
  • GENE METHYLATION IN CANCER DIAGNOSIS
    Publication number: 20120100541
    Abstract: The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use in detecting and diagnosing cancer.
    Type: Application
    Filed: October 24, 2011
    Publication date: April 26, 2012
    Applicant: Orion Genomics LLC
    Inventors: Jared Ordway, Jeffrey A. Jeddeloh, Joseph Bedell
  • METHODS FOR QUANTITATIVE DETERMINATION OF METHYLATION DENSITY IN A DNA LOCUS
    Publication number: 20120100535
    Abstract: The present invention is a novel method of determining the average DNA methylation density of a locus of interest within a population of DNA fragments.
    Type: Application
    Filed: October 1, 2010
    Publication date: April 26, 2012
    Applicant: Orion Genomics LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey
  • Differential enzymatic fragmentation
    Patent number: 8163485
    Abstract: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids.
    Type: Grant
    Filed: June 1, 2010
    Date of Patent: April 24, 2012
    Assignee: Orion Genomics, LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey
  • METHODS AND SYSTEMS FOR UNIFORM ENRICHMENT OF GENOMIC REGIONS
    Publication number: 20120071357
    Abstract: The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.
    Type: Application
    Filed: October 7, 2011
    Publication date: March 22, 2012
    Inventors: Jacob Kitzman, Todd Richmond, Mark D'Ascanzo, Thomas Albert, Jeffrey Jeddeloh, Christina Middle
  • Differential enzymatic fragmentation by whole genome amplification
    Patent number: 8088581
    Abstract: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids.
    Type: Grant
    Filed: October 10, 2008
    Date of Patent: January 3, 2012
    Assignee: Orion Genomics LLC
    Inventors: Nathan D. Lakey, Jeffrey A. Jeddeloh, Yulia Korshunova
  • Gene methylation in cancer diagnosis
    Patent number: 8067168
    Abstract: The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use in detecting and diagnosing cancer.
    Type: Grant
    Filed: May 30, 2007
    Date of Patent: November 29, 2011
    Assignee: Orion Genomics LLC
    Inventors: Jared Ordway, Jeffrey A. Jeddeloh, Joseph Bedell
  • Gene methylation in cancer diagnosis
    Patent number: 7960112
    Abstract: The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of cancer.
    Type: Grant
    Filed: February 1, 2008
    Date of Patent: June 14, 2011
    Assignee: Orion Genomics LLC
    Inventors: Muhammad A. Budiman, Jeffrey A. Jeddeloh, Rebecca Maloney, Yulia Korshunova
  • Differential enzymatic fragmentation by whole genome amplification
    Publication number: 20110076726
    Abstract: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids.
    Type: Application
    Filed: October 10, 2008
    Publication date: March 31, 2011
    Applicant: Orion Genomics LLC
    Inventors: Nathan D. Lakey, Jeffrey A. Jeddeloh, Yulia Korshunova
  • Methods for quantitative determination of methylation density in a DNA locus
    Patent number: 7910296
    Abstract: The present invention is a novel method of determining the average DNA methylation density of a locus of interest within a population of DNA fragments.
    Type: Grant
    Filed: October 21, 2004
    Date of Patent: March 22, 2011
    Assignee: Orion Genomics LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey
  • Differential enzymatic fragmentation
    Patent number: 7901880
    Abstract: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids.
    Type: Grant
    Filed: October 21, 2004
    Date of Patent: March 8, 2011
    Assignee: Orion Genomics LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey
  • System and method for improved processing of nucleic acids for production of sequencable libraries
    Publication number: 20110003701
    Abstract: An embodiment of an adaptor element for efficient target processing is described that comprises a semi-complementary double stranded nucleic acid adaptor comprising a non-complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site and a second amplification primer site and the complementary region comprises a sequencing primer site and one or more inosine species.
    Type: Application
    Filed: February 23, 2009
    Publication date: January 6, 2011
    Inventors: Gianni Calogero Ferreri, Jan F. Simons, Michael Todd Ronan, Michael Egholm, Brian C. Godwin, David Roderick Riches, Stephen Kyle Hutchison, Michael S. Braverman, Melinda D. Palmer, Jeffrey Jeddeloh, Jacob Kitzman, Thomas J. Albert
  • DIFFERENTIAL ENZYMATIC FRAGMENTATION
    Publication number: 20100240064
    Abstract: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids.
    Type: Application
    Filed: June 1, 2010
    Publication date: September 23, 2010
    Applicant: Orion Genomics LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey
  • ENRICHMENT AND SEQUENCE ANALYSIS OF GENOMIC REGIONS
    Publication number: 20100222232
    Abstract: The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules.
    Type: Application
    Filed: April 5, 2010
    Publication date: September 2, 2010
    Inventors: Thomas J. Albert, Roland Green, Todd Richmond, Michael Molla, Jeffrey Jeddeloh, Jason Patrick Affourtit, Mathreyan Srinivasan, Brian Christopher Godwin, Matthew Rodesch
  • Differential enzymatic fragmentation
    Patent number: 7771936
    Abstract: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids.
    Type: Grant
    Filed: October 21, 2004
    Date of Patent: August 10, 2010
    Assignee: Orion Genomics LLC
    Inventors: Jeffrey A. Jeddeloh, Nathan D. Lakey