Abstract: A method of producing biogenic silica nanoparticles comprises pretreating seed hulls of a biogenic source with an acid to form acid-treated seed hulls; placing the acid-treated seed hulls in an autoclave at a temperature greater than 100° C. for about 2 hours under a fixed pressure; isolating the seed hulls; washing the seed hulls with water; air drying the seed hulls; calcining the seed hulls at a temperature range of 500° C. to 700° C. for at least one hour in a furnace to produce biogenic silica nanoparticles. The biogenic silica nano-particles are amorphous and biocompatible possessing a particle sizes in the range of 25-75 nm.

Abstract: The method of making a three-dimensional scaffold for three-dimensional cell culture includes obtaining amorphous silica from sorghum husks, producing biogenic silica microbodies from the amorphous silica, and clustering the biogenic silica microbodies to form the three-dimensional scaffold. The three-dimensional scaffold can be used for growing three-dimensional cell cultures, such as human mesenchymal stem cell cultures.