Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Abstract: Provided herein are methods, compositions, and kits for determining a location of a target analyte in a biological sample that include the use of terminal deoxynucleotidyl transferase.

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

Abstract: Provided herein are methods for determining a location of a protein in a biological sample including: disposing the sample onto an array comprising a plurality of capture probes, where a first and second capture probe include a first and second spatial barcode, respectively, and a capture domain. The second capture probe is not covered by the sample and is contacted with a solution comprising TdT and one or more dideoxynucleotides, where a dideoxynucleotide is incorporated into the second capture domain. Analyte capture agents comprising an analyte binding moiety that binds protein and an oligonucleotide including an analyte capture sequence and an analyte binding moiety barcode are contacted with the sample. The analyte capture sequence hybridizes to the first capture domain, and the sequence of the first spatial barcode and the analyte binding moiety barcode, or complements thereof, are used to determine the location of the protein in the biological sample.

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

Abstract: Provided herein are methods, compositions, and kits for determining a location of a target analyte in a biological sample that include the use of terminal deoxynucleotidyl transferase.

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Abstract: Provided herein are methods and kits for binding of an analyte capture sequence to a capture domain of a capture probe.

Abstract: Provided are methods for determining a location of a target nucleic acid in a biological sample including: disposing the biological sample onto an array including a plurality of capture probes, where a first capture probe includes a first spatial barcode and a capture domain and a second capture probe includes a second spatial barcode and the capture domain. The second capture probe is not covered by the biological sample on the array and is contacted with a solution comprising TdT and one or more dideoxynucleotides, such that a dideoxynucleotide is incorporated into the second capture domain. Target nucleic acids are captured by the first capture probe, and the sequence of the first spatial barcode or a complement thereof and all or a portion of a sequence of the target nucleic acid, or a complement thereof, are used to determine the location of the target nucleic acid in the biological sample.

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Abstract: Provided herein are methods of releasing an extended capture probe from a substrate and uses of the same.

Abstract: Provided herein are methods, compositions, and kits for determining a location of a target analyte in a biological sample that include the use of terminal deoxynucleotidyl transferase.

Abstract: Provided herein are methods for resetting an array to which a biological sample has been applied that include treating the array with a set of biological sample removal conditions.

Abstract: Provided herein are methods of identifying the spatial location of a nucleic acid in a biological sample. In some embodiments, the methods employ a template switching oligonucleotide. In some embodiments, the methods extend the capture probe to create a complementary DNA (cDNA) molecule of a capture analyte and produce second strand in one reaction.

Abstract: Provided herein are methods for capturing an analyte from a first region of interest of a biological sample on a substrate, where the biological sample comprises the first region of interest and a second region, and where the method includes contacting the second region with a sealant in order to create a hydrophobic seal thereby preventing an interaction between an analyte from the second region with a capture domain of a capture probe.

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Abstract: Provided herein are methods for resetting an array to which a biological sample has been applied that include treating the array with a set of biological sample removal conditions.

Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.