Patents by Inventor Ji Yoon Kang
Ji Yoon Kang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11944661Abstract: The present invention provides a pharmaceutical composition for prevention or treatment of a stress disease and depression, the pharmaceutical composition be safely useable without toxicity and side effects by using an extract of leaves of Vaccinium bracteatum Thunb., which is natural resource of Korea, so that the reduction of manufacturing and production costs and the import substitution and export effects can be expected through the replacement of a raw material for preparation with a plant inhabiting in nature.Type: GrantFiled: February 7, 2018Date of Patent: April 2, 2024Assignee: JEONNAM BIOINDUSTRY FOUNDATIONInventors: Chul Yung Choi, Dool Ri Oh, Yu Jin Kim, Eun Jin Choi, Hyun Mi Lee, Dong Hyuck Bae, Kyo Nyeo Oh, Myung-A Jung, Ji Ae Hong, Kwang Su Kim, Hu Won Kang, Jae Yong Kim, Sang O Pan, Sung Yoon Park, Rack Seon Seong
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Publication number: 20240060993Abstract: Provided is a method by which a biosensor device detects an antigen by using multiple frequencies, the method enabling an antigen to be detected with high sensitivity. The method by which a biosensor device detects an antigen by using multiple frequencies includes: loading an antibody in the biosensor device including a micro-well that has an action electrode and a counterpart electrode disposed therein; measuring the impedance of the antibody for each of a first frequency and a second frequency; performing an antigen-antibody reaction by injecting an antigen into the biosensor device; using the first frequency and the second frequency to measure the impedances of each of the antigen and the antibody that are bound by means of the antigen-antibody reaction; and calculating changes in impedance that result from the antigen-antibody reaction from the impedances of the antigen and the antibody for the first frequency and the impedances of the antigen and the antibody for the second frequency.Type: ApplicationFiled: December 28, 2021Publication date: February 22, 2024Inventors: Kyeong Sik SHIN, Ji Yoon KANG
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Patent number: 11668709Abstract: The present disclosure relates to a system for monitoring post-translational modification of protein using a biosensor with a gap, which performs with high reliability a diagnosis of a disease associated with a target protein for which impedance is measured, by measuring an impedance of a sample introduced into a sensor and calculating a change rate of the measured impedance, and to a method of manufacturing the biosensor used for the system.Type: GrantFiled: September 24, 2019Date of Patent: June 6, 2023Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Soo Hyun Lee, Ji Yoon Kang, Hyewhon Rhim, Yi Jae Lee, Hyewon Seo, Yun Kyung Kim
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Patent number: 11320442Abstract: Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.Type: GrantFiled: April 19, 2017Date of Patent: May 3, 2022Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon Kang, Min Cheol Park, Youhee Heo
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Patent number: 11313828Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.Type: GrantFiled: January 13, 2016Date of Patent: April 26, 2022Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon Kang, Kyeong-Sik Shin, Jae-hoon Ji, Kyoseon Hwang, Tae Song Kim
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Patent number: 11054427Abstract: Disclosed is a method of quantifying a biomarker with high sensitivity using photo-oxidation induced amplification. The method includes performing an enzyme-substrate reaction of a measurement sample including an enzyme labeled on any one selected from among an antibody, an aptamer, and a nucleic acid specifically bound to the biomarker, optically measuring one or more optical properties selected from among amounts of color formation, light emission, and fluorescence of a product during a photo-oxidation induced amplification process while the product resulting from the enzyme-substrate reaction is continuously exposed to light to thus perform the photo-oxidation induced amplification process, indexing a time-varying pattern of the measured optical properties, and quantifying a concentration of the biomarker included in the measurement sample by comparing an index extracted during the indexing with an index of a reference sample.Type: GrantFiled: March 21, 2017Date of Patent: July 6, 2021Assignee: ABSOLOGY CO., LTDInventors: Ji Yoon Kang, Min Cheol Park, Youhee Heo
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Publication number: 20200188918Abstract: The present invention relates to a method for isolating extracellular vesicles comprising: preparing a microfluidic chip in which at least two microfluidic channels are formed and inlets and outlets are formed at both ends of the channels, respectively; injecting at least one aqueous solution into the inlet of the microfluidic chip to form a microfluid; injecting a sample containing extracellular vesicles into the inlet of the microfluidic chip; and recovering the fluid from the outlet of the microfluidic chip.Type: ApplicationFiled: November 13, 2019Publication date: June 18, 2020Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon KANG, Bo Hoon HAN, You hee HEO, Kyeong Sik SHIN
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Patent number: 10648988Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.Type: GrantFiled: January 23, 2018Date of Patent: May 12, 2020Assignee: Korea Institute of Science and TechnologyInventors: Ji Yoon Kang, Kyeong-Sik Shin, Jae-hoon Ji, Youhee Heo, Kyoseon Hwang, Myung-Sic Chae
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Publication number: 20200110099Abstract: The present disclosure relates to a system for monitoring post-translational modification of protein using a biosensor with a gap, which performs with high reliability a diagnosis of a disease associated with a target protein for which impedance is measured, by measuring an impedance of a sample introduced into a sensor and calculating a change rate of the measured impedance, and to a method of manufacturing the biosensor used for the system.Type: ApplicationFiled: September 24, 2019Publication date: April 9, 2020Inventors: Soo Hyun Lee, Ji Yoon KANG, Hyewhon RHIM, Yi Jae LEE, Hyewon SEO, Yun Kyung KIM
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Publication number: 20200088743Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.Type: ApplicationFiled: November 15, 2019Publication date: March 19, 2020Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Soo-Hyun LEE, Ji-Yoon KANG, Min-Ho KIM, Yun-Kyung KIM
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Patent number: 10585105Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.Type: GrantFiled: May 18, 2016Date of Patent: March 10, 2020Assignee: Korea Institute of Science and TechnologyInventors: Ji Yoon Kang, Kyeong Sik Shin, Jae Hoon Ji, Tae Song Kim, Young Soo Kim, Sang Yun Kim
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Patent number: 10585022Abstract: Provided are an extracellular vesicle collecting apparatus using an electrode and a porous membrane and a method for using the same. The extracellular vesicle collecting apparatus includes i) an upper frame in which a pair of through holes is disposed so that a buffer solution flows therethrough, ii) a buffer tube which is inserted in the through holes and in which the buffer solution flows, iii) a positive electrode disposed on the upper frame, iv) a porous membrane located below the upper frame, v) a spacer which is located below the porous membrane and has a hollow space disposed therein, vi) a plurality of guide tubes through which a blood flows in the hollow space, vii) a lower frame which is coupled to the upper frame to be opposite to the upper frame and receives the porous membrane and the spacer therein, and viii) a negative electrode provided below the lower frame. Extracellular vesicles contained in the blood are collected by the porous membrane.Type: GrantFiled: July 22, 2016Date of Patent: March 10, 2020Assignee: Korea Institute of Science and TechnologyInventors: Rhokyun Kwak, Ji Yoon Kang, Jaesung Park, Siwoo Cho
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Patent number: 10520514Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.Type: GrantFiled: September 6, 2017Date of Patent: December 31, 2019Assignee: Korea Institute of Science and TechnologyInventors: Soo-Hyun Lee, Ji-Yoon Kang, Min-Ho Kim, Yun-Kyung Kim
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Publication number: 20180238910Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.Type: ApplicationFiled: January 23, 2018Publication date: August 23, 2018Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon KANG, Kyeong-Sik SHIN, Jae-hoon JI, Youhee HEO, Kyoseon HWANG, Myung-Sic CHAE
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Publication number: 20180217164Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.Type: ApplicationFiled: September 6, 2017Publication date: August 2, 2018Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Soo-Hyun LEE, Ji-Yoon KANG, Min-Ho KIM, Yun-Kyung KIM
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Publication number: 20180180621Abstract: Disclosed is a method of quantifying a biomarker with high sensitivity using photo-oxidation induced amplification. The method includes performing an enzyme-substrate reaction of a measurement sample including an enzyme labeled on any one selected from among an antibody, an aptamer, and a nucleic acid specifically bound to the biomarker, optically measuring one or more optical properties selected from among amounts of color formation, light emission, and fluorescence of a product during a photo-oxidation induced amplification process while the product resulting from the enzyme-substrate reaction is continuously exposed to light to thus perform the photo-oxidation induced amplification process, indexing a time-varying pattern of the measured optical properties, and quantifying a concentration of the biomarker included in the measurement sample by comparing an index extracted during the indexing with an index of a reference sample.Type: ApplicationFiled: March 21, 2017Publication date: June 28, 2018Inventors: Ji Yoon KANG, Min Cheol PARK, Youhee HEO
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Publication number: 20180080946Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.Type: ApplicationFiled: May 18, 2016Publication date: March 22, 2018Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon KANG, Kyeong Sik SHIN, Jae Hoon JI, Tae Song KIM, Young Soo KIM, Sang Yun KIM
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Publication number: 20170307638Abstract: Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.Type: ApplicationFiled: April 19, 2017Publication date: October 26, 2017Inventors: Ji Yoon KANG, Min Cheol PARK, Youhee HEO
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Publication number: 20170131233Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.Type: ApplicationFiled: January 13, 2016Publication date: May 11, 2017Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon Kang, Kyeong-Sik Shin, Jae-hoon Ji, Kyoseon Hwang, Tae Song Kim
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Publication number: 20170045430Abstract: Provided are an extracellular vesicle collecting apparatus using an electrode and a porous membrane and a method for using the same. The extracellular vesicle collecting apparatus includes i) an upper frame in which a pair of through holes is disposed so that a buffer solution flows therethrough, ii) a buffer tube which is inserted in the through holes and in which the buffer solution flows, iii) a positive electrode disposed on the upper frame, iv) a porous membrane located below the upper frame, v) a spacer which is located below the porous membrane and has a hollow space disposed therein, vi) a plurality of guide tubes through which a blood flows in the hollow space, vii) a lower frame which is coupled to the upper frame to be opposite to the upper frame and receives the porous membrane and the spacer therein, and viii) a negative electrode provided below the lower frame. Extracellular vesicles contained in the blood are collected by the porous membrane.Type: ApplicationFiled: July 22, 2016Publication date: February 16, 2017Applicant: Korea Institute of Science and TechnologyInventors: Rhokyun KWAK, Ji Yoon KANG, Jaesung PARK, Siwoo CHO