Abstract: The present application relates to a gas adsorption concentration apparatus, including an adsorption section and a regeneration section. The adsorption section includes two or more adsorption modules each provided with a plurality of stages of adsorption units, the adsorption unit is configured as a fixed adsorption bed provided with an adsorbent, and when an adsorption gas containing adsorbates and basic components passes through the adsorption module, the adsorbates are adsorbed by the adsorbent in the adsorption unit. The regeneration section includes at least one desorption module provided with a plurality of stages of adsorption units, and when a hot desorption gas passes through the desorption module, the adsorbates in the adsorption unit are desorbed and carried out by the desorption gas. The adsorption units are transferred between the adsorption section and the regeneration section by means of an adsorption unit moving apparatus and circulate in adsorption and desorption processes.

Abstract: A vehicle-based communication method, a vehicle and a storage medium are provided. The method is applied to a vehicle, the vehicle includes a sending end and a receiving end of messages, and the method includes the steps that the sending end determines message information containing multiple pieces of semaphore information; the sending end determines a length of placeholder data of a semaphore and a bit field data structure containing the placeholder data according to the message information and the preset message data length, where the length of the placeholder data is determined according to the start position and the data length of a respective semaphore; and the sending end packs multiple semaphores based on the bit field data structure to obtain a target message, and sends the target message to the receiving end.

Abstract: The present invention provides a compound having a new structure as a PDE4B inhibitor, a preparation method for the compound, and a use of the compound in treating PDE4B-mediated diseases. Experiments confirm that the compound has a strong inhibitory effect on PDE4B enzyme and can be used as a promising compound for treating PDE4B-mediated diseases.

Abstract: The present invention relates to the field of nucleic acid editing, in particular to the field of clustered regularly interspaced short palindromic repeat (CRISPR) technology. Specifically, the present invention relates to Cas effector proteins, fusion proteins comprising such proteins, and nucleic acid molecules encoding them. The present invention also relates to complexes and compositions for nucleic acid editing (e.g., gene or genome editing), which comprise the proteins or fusion proteins of the present invention, or nucleic acid molecules encoding them. The present invention also relates to a method for nucleic acid editing (e.g., gene or genome editing), which uses the proteins or fusion proteins comprising the present invention.

Abstract: The invention provides the construction and use of a biosensor screening system for lactose or an analog thereof in Bacillus subtilis. The present disclosure designs a “dual-selection” gene circuit for screening biosensor repressor proteins based on lactose operons and applicable to Bacillus subtilis. The DNA-binding activity of the repressor proteins is screened via a lethal circuit under negative selection, and the allosteric activation activity screening is achieved based on a fluorescence signal under positive selection. Ultimately, biosensors with recognition activity for target lactose analogs are obtained. A novel biosensor responsive to substances such as lactose, 2?-fucosyllactose, and 3-fucosyllactose can be obtained by using the screening platform, and it has been demonstrated that the biosensor constructed based on the screening results in the present disclosure exhibits relatively high detection sensitivity and specificity.

Abstract: The present invention provides a high-throughput method for screening ?-1,2-fucosyltransferase and a screened mutant. The method includes the steps of constructing an indicator strain of 2?-fucosyllactose, so that the content of 2?-fucosyllactose is positively correlated with the fluorescence intensity, then expressing L-fucokinase and an ?-1,2-fucosyltransferase mutant library in a chassis strain, embedding the chassis strain in water-in-oil droplets and fusing and co-culturing with the indicator strain, and obtaining a chassis strain comprising a forward mutant of ?-1,2-fucosyltransferase by microfluidic sorting of droplets according to the fluorescence detection results. The enzyme activity of the mutant FutCV93I is 2.31 times that of the original enzyme, so the enzyme activity is greatly enhanced.

Abstract: A method, apparatus, non-transitory computer readable medium, and system for data processing includes receiving a document and a query requesting information about the document, identifying, using a language generation model, a relevant page of the document based on the query, and generating, using the language generation model, a response to the query based on the relevant page, wherein the response incudes the requested information about the document.

Abstract: The invention provides a multi-site dual-function base editor and use thereof. Dual editing of multiple sites under the guidance of a single crRNA array is realized by using a constructed single-plasmid editor backbone, through the composition and structure optimization of a fusion protein formed of a cytosine deaminase, UGI, an adenine deaminase, and dCas12a. On this basis, the multi-site editing efficiency is further improved through the promoter replacement, the introduction and optimization of a synthetic spacing sequence in the crRNA array and the modification of a DR motif. Finally, the function of the multi-site dual-function base editor is verified through the generation of a multi-resistance strain, the de novo generation of a riboflavin producing strain, and the increased yield of a surfactin producing strain. According to the invention, the operable range of the dual-function base editor on the genome is widened.

Abstract: An anti-backflow apparatus includes a cup, a protective cover, and a gas exhaust unit. The upper portion of the cup is provided with an opening. The protective cover covers the opening of the cup and is provided with a liquid receiving port and a gas exhaust port. The liquid receiving port is used for a nozzle to drip chemical liquid into the cup. The gas exhaust unit is connected to the inside of the cup through the gas exhaust port of the protective cover, so as to suck volatile gas inside the cup, which can effectively prevent the volatile gas inside the cup from flowing back into a process chamber of a substrate processing device, and can prevent the volatile gas from corroding the surface of a substrate in the process chamber.

Abstract: Provided is a method for processing a user query, an electronic device and a storage medium, relating to the field of computer technology, and in particular to the fields of artificial intelligence, large language model and other technologies. The method includes: receiving a first user query; performing word segmentation on the first user query to obtain a token list corresponding to the first user query; determining a reusable key-value cache of the first user query according to the token list; and when the reusable key-value cache is in a process of transmission from a GPU to a CPU, stopping the transmission of the reusable key-value cache, and allocating the reusable key-value cache to the first user query.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: An electronic lock configured with internal and external modules includes a first module and a second module installed on inner and outer sides of a door leaf, respectively. The first module includes a first housing and a first circuit board accommodated in the first housing, and the second module includes a second housing and a second circuit board accommodated in the second housing. The first module further includes a first conductive contact arranged on the first circuit board and a first conductive post electrically signal-connected to the first conductive contact, and the second module further includes a second conductive contact arranged on the second circuit board and a second conductive post electrically signal-connected to the second conductive contact; and one of the first conductive post and the second conductive post is formed with an insertion cavity, and the other conductive post can be inserted into the insertion cavity.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: The present invention provides an efficient ultrasonic phased array phase shift migration imaging method for the defect in a multi-layer structure, including the following steps: (1) transforming full matrix capture data of a multi-layer structure to a frequency-wavenumber domain by means of three-dimensional fast Fourier transform; (2) for any non-traversed layer in the multi-layer structure, extrapolating a surface wave field of the multi-layer structure to an upper interface of the non-traversed layer, to obtain wave field information of the non-traversed layer; (3) according to the obtained wave field information, performing focus imaging on the non-traversed layer in the frequency-wavenumber domain; and (4) repeating the steps (2) and (3), until all layers are traversed, to obtain an imaging result of the multi-layer structure.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: The present invention relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). In particular, the present invention relates to a Type I-A CRISPR-Cas effector protein and system, a fusion protein comprising the protein, and nucleic acid molecules encoding same. The present invention also relates to a complex and composition for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single base editing, genomic structural variation), which comprises the protein or fusion protein of the present invention, or nucleic acid molecules encoding same. The present invention further relates to a method for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single base editing, genomic structural variation), which uses the protein or fusion protein comprised in the present invention.