Abstract: The invention provides a multi-site dual-function base editor and use thereof. Dual editing of multiple sites under the guidance of a single crRNA array is realized by using a constructed single-plasmid editor backbone, through the composition and structure optimization of a fusion protein formed of a cytosine deaminase, UGI, an adenine deaminase, and dCas12a. On this basis, the multi-site editing efficiency is further improved through the promoter replacement, the introduction and optimization of a synthetic spacing sequence in the crRNA array and the modification of a DR motif. Finally, the function of the multi-site dual-function base editor is verified through the generation of a multi-resistance strain, the de novo generation of a riboflavin producing strain, and the increased yield of a surfactin producing strain. According to the invention, the operable range of the dual-function base editor on the genome is widened.

Abstract: A method, apparatus, non-transitory computer readable medium, and system for data processing includes receiving a document and a query requesting information about the document, identifying, using a language generation model, a relevant page of the document based on the query, and generating, using the language generation model, a response to the query based on the relevant page, wherein the response incudes the requested information about the document.

Abstract: An anti-backflow apparatus includes a cup, a protective cover, and a gas exhaust unit. The upper portion of the cup is provided with an opening. The protective cover covers the opening of the cup and is provided with a liquid receiving port and a gas exhaust port. The liquid receiving port is used for a nozzle to drip chemical liquid into the cup. The gas exhaust unit is connected to the inside of the cup through the gas exhaust port of the protective cover, so as to suck volatile gas inside the cup, which can effectively prevent the volatile gas inside the cup from flowing back into a process chamber of a substrate processing device, and can prevent the volatile gas from corroding the surface of a substrate in the process chamber.

Abstract: Provided is a method for processing a user query, an electronic device and a storage medium, relating to the field of computer technology, and in particular to the fields of artificial intelligence, large language model and other technologies. The method includes: receiving a first user query; performing word segmentation on the first user query to obtain a token list corresponding to the first user query; determining a reusable key-value cache of the first user query according to the token list; and when the reusable key-value cache is in a process of transmission from a GPU to a CPU, stopping the transmission of the reusable key-value cache, and allocating the reusable key-value cache to the first user query.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: An electronic lock configured with internal and external modules includes a first module and a second module installed on inner and outer sides of a door leaf, respectively. The first module includes a first housing and a first circuit board accommodated in the first housing, and the second module includes a second housing and a second circuit board accommodated in the second housing. The first module further includes a first conductive contact arranged on the first circuit board and a first conductive post electrically signal-connected to the first conductive contact, and the second module further includes a second conductive contact arranged on the second circuit board and a second conductive post electrically signal-connected to the second conductive contact; and one of the first conductive post and the second conductive post is formed with an insertion cavity, and the other conductive post can be inserted into the insertion cavity.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: The present invention provides an efficient ultrasonic phased array phase shift migration imaging method for the defect in a multi-layer structure, including the following steps: (1) transforming full matrix capture data of a multi-layer structure to a frequency-wavenumber domain by means of three-dimensional fast Fourier transform; (2) for any non-traversed layer in the multi-layer structure, extrapolating a surface wave field of the multi-layer structure to an upper interface of the non-traversed layer, to obtain wave field information of the non-traversed layer; (3) according to the obtained wave field information, performing focus imaging on the non-traversed layer in the frequency-wavenumber domain; and (4) repeating the steps (2) and (3), until all layers are traversed, to obtain an imaging result of the multi-layer structure.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.

Abstract: The present invention relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). In particular, the present invention relates to a Type I-A CRISPR-Cas effector protein and system, a fusion protein comprising the protein, and nucleic acid molecules encoding same. The present invention also relates to a complex and composition for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single base editing, genomic structural variation), which comprises the protein or fusion protein of the present invention, or nucleic acid molecules encoding same. The present invention further relates to a method for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single base editing, genomic structural variation), which uses the protein or fusion protein comprised in the present invention.

Abstract: A children's dining chair includes a base, handles, and a backrest. A left side, a right side, and a rear side of the base are provided with blocking walls. A first storage slot is defined in the blocking walls. The handles are detachably arranged on the left side and the right side of the base. The backrest is detachably arranged on the rear side of the base. When the handles and backrest are disassembled from the base, the handles and the backrest are capable of being stored in the first storage slot.

Abstract: This application relates to the field of power tool technologies, and specifically discloses a power tool and a control method thereof. The tool includes at least two output heads. An output head detection assembly can detect a position of an output head at a working position and generate a detection signal. The detection signal is associated with a working mode of the output head. A function switching unit generates, in response to external triggering, a mode switching signal used for indicating to switch the working mode of the output head at the working position. A controller is connected to the output head detection assembly and the function switching unit, switches the working mode of the output head at the working position in response to the mode switching signal, and associates the detection signal with a working mode after switching.

Abstract: A lockset includes symmetrically arranged front and rear handle assemblies, where the rear handle assembly includes a rear fixing base and a rear handle body. The rear fixing base is mounted on a door leaf. A connecting rod is configured to connect the front and rear assemblies. With a head facing rearward, a first fastening screw is provided outside the rear handle body to lock and connect the rear fixing base to the connecting rod. The rear handle body is provided with a through hole corresponding to the fastening screw. A movable cover body is configured to cover or expose the through hole for convenient operation. The rear handle body includes a supporting shaft and a housing. The housing and the cover body together form a gripping portion. The through hole is provided in the housing. An avoidance groove is provided on an outer side of the supporting shaft.

Abstract: This application discloses a battery tray and a battery formation and capacity-grading device. The battery tray includes a fixed frame; and a fixed component located in the fixed frame, the fixed component including a first limiting assembly and a second limiting assembly, the first limiting assembly including at least two first limiting members which extend along a first direction respectively and are spaced apart along a second direction which intersects the first direction, the second limiting assembly being connected to the first limiting assembly and including at least two second limiting members which are disposed along the first direction and are connected with the first limiting members to form a limiting space for accommodating a battery cell, and the second limiting members being movably disposed along the first limiting members in the first direction.

Abstract: The present invention relates to the field of nucleic acid editing, in particular to the field of clustered regularly interspaced short palindromic repeat (CRISPR) technology. Specifically, the present invention relates to Cas effector proteins, fusion proteins comprising such proteins, and nucleic acid molecules encoding them. The present invention also relates to complexes and compositions for nucleic acid editing (e.g., gene or genome editing), which comprise the proteins or fusion proteins of the present invention, or nucleic acid molecules encoding them. The present invention also relates to a method for nucleic acid editing (e.g., gene or genome editing), which uses the proteins or fusion proteins comprising the present invention.

Abstract: The application discloses a switching power supply with constant current output and a method for controlling constant current of a switching power supply, wherein the switching power supply comprises a detection circuit and a logic control chip, and the detection circuit is configured to output a feedback voltage. The feedback voltage is a sum voltage of a first voltage and a second voltage, and the second voltage represents output current of the switching power supply. The logic control chip is configured to receive the feedback voltage and compare the feedback voltage with a reference voltage. When the output current changes, the logic control chip changes turn on time of the switching power supply based on comparison result to control the output current constant.