Abstract: The present disclosure belongs to the technical field of analytical chemistry, in particular to synthesis and application of a nanomaterial for removal of patulin (Pat). The present disclosure adopts 2-Oxin as a substitute template, AM as a functional monomer, and synthetic Fe3O4@SiO2@CS-GO magnetic nanoparticles as a carrier, for preparing a magnetic MIP specific for Pat adsorption by surface imprinting. The addition of Fe3O4 makes the finally prepared molecular imprinted adsorbent material magnetic, thereby facilitating separation of a material from a matrix, eliminating complicated operation steps such as filtration and centrifugation, and facilitating recovery of materials.

Abstract: The present invention discloses a time-resolved immunoquantitation test strip for detecting tetrodotoxin (TTX) in shellfish food, which belongs to the technical field of rapid detection of time-resolved immunoassay. In the present invention, fluorescent microspheres are adopted to replace the traditional colloidal gold; the fluorescent microsphere is used for labeling a TTX antibody complex; by utilizing a competitive immunization method, the fluorescent microsphere, serving as a fluorescent probe, is used for immunochromatography; and by reading the fluorescence value of a detection line on a fluoroimmunoassay instrument, the TTX in shellfish samples can be analyzed quantitatively and rapidly. The time-resolved immunoquantitation test strip of the present invention can be used for detecting the content of the TTX in various types of shellfish food quantitatively and rapidly and is strong in specificity and high in sensitivity, wherein when the concentration of the TTX is 0.

Abstract: A polyelectrolyte hydrogel coating with strong substrate binding performance and a method of manufacturing the same are provided. The method includes: 1) activating a substrate by applying oxygen plasma; and 2) dissolving a polycationic polymer, a polymeric monomer, a silane coupling agent and an initiator to obtain a precursor solution, vacuumizing the precursor solution to remove air bubbles, applying the vacuumized precursor solution to a surface of the substrate activated by the oxygen plasma, and performing an in-situ polymerization and curing process under nitrogen or rare gas atmosphere. The polymeric monomer includes at least one of: acrylamide, acrylic acid, hydroxyethyl methacrylamide, 2-acrylamido-2-methyl-1-propanesulfonic acid, polyethylene glycol (diol) diacrylate, 2-methacryloxyethylphosphocholine, 3-[[2-(Methacryloyloxy)ethyl]dimethylammonio]propane-1-sulfonate, and 3-[[2-(methacryloyloxy)ethyl]dimethylammonium] propionate.

Abstract: The disclosure provides a single-cell-based electrochemical sensor based on a functionalized nano-probe and an application thereof, and belongs to the technical fields of electrochemical sensors and toxin detection. The single-cell-based electrochemical sensor of the disclosure combines a nano-probe and an electrochemical cell-based sensor, conducts functional modification on the nano-probe using Prussian blue, and conducts current signal analysis on a single cell by a micro-operating platform. The disclosure constructs a reliable, easy to operate and highly repeatable single-cell-based electrochemical detection platform, and the current value is determined by electrochemical chronoamperometry to determine damage of a single cell stimulated by toxins, thereby quickly and effectively evaluating the cytotoxicity of fungal toxins, and further enabling application of the fungal toxin toxicity in real-time monitoring and nano-environmental detection in living cells.

Abstract: A method, system, and computer program product for detecting and monitoring a signal is provided. The method includes detecting an alignment point for a periodic signal segment of a periodic signal generated by an apparatus being monitored for standard functionality In response, the apparatus is activated from a period prior to the alignment point to an end point of the periodic signal segment and a first point of the periodic signal segment is located. Likewise, a second point of an additional periodic signal segment of the periodic signal is located. The periodic signal is normalized based on results of locating the first point and the second point.

Abstract: Embodiments of the invention are directed to a computer-implemented method that includes determining, by a controller of a messaging system, a cognitive context of a message sender. The method also includes configuring at least one property of a message based on the determined cognitive context. The message is transmitted by the message sender to a first receiver. The method also includes transmitting the message to a second receiver based on the at least one configured property.

Abstract: A method, system, and computer program product for detecting and monitoring a signal is provided. The method includes detecting an alignment point for a periodic signal segment of a periodic signal generated by an apparatus being monitored for standard functionality In response, the apparatus is activated from a period prior to the alignment point to an end point of the periodic signal segment and a first point of the periodic signal segment is located. Likewise, a second point of an additional periodic signal segment of the periodic signal is located. The periodic signal is normalized based on results of locating the first point and the second point.

Abstract: Blockchain-based file handling is provided by receiving a data file from a user device, storing the data file to local storage of the blockchain peer, generating a file identifier of the data file, providing the file identifier to the user device, storing the file identifier to a synchronized ledger of the blockchain network, where the synchronized ledger tracks access to the data file, and distributing data of the data file to one or more other blockchain peers of the blockchain network.

Abstract: The disclosure relates to a cell electrochemical sensor based on a 3D printing technology and application thereof and belongs to the technical field of electrochemical sensors and toxin detection. The cell electrochemical sensor of the disclosure is constructed based on a 3D printing technology, and the construction method comprises the following steps: precisely depositing a cell/carbon nanofiber/GelMA composite hydrogel on a working electrode of a screen-printed carbon electrode through 3D printing, and carrying out curing to obtain the cell electrochemical sensor. The disclosure constructs a cell electrochemical sensor with a three-dimensional cell growth environment and rapid and sensitive response. The cell electrochemical sensor constructed by the disclosure can be used for quickly and effectively determining the combined effect type and effect degree of deoxynivalenol family toxins by combining an electrochemical impedance method and a combination index method.

Abstract: Blockchain-based file handling is provided by receiving a data file from a user device, storing the data file to local storage of the blockchain peer, generating a file identifier of the data file, providing the file identifier to the user device, storing the file identifier to a synchronized ledger of the blockchain network, where the synchronized ledger tracks access to the data file, and distributing data of the data file to one or more other blockchain peers of the blockchain network.

Abstract: The disclosure relates to a cell electrochemical sensor based on a 3D printing technology and application thereof and belongs to the technical field of electrochemical sensors and toxin detection. The cell electrochemical sensor of the disclosure is constructed based on a 3D printing technology, and the construction method comprises the following steps: precisely depositing a cell/carbon nanofiber/GelMA composite hydrogel on a working electrode of a screen-printed carbon electrode through 3D printing, and carrying out curing to obtain the cell electrochemical sensor. The disclosure constructs a cell electrochemical sensor with a three-dimensional cell growth environment and rapid and sensitive response. The cell electrochemical sensor constructed by the disclosure can be used for quickly and effectively determining the combined effect type and effect degree of deoxynivalenol family toxins by combining an electrochemical impedance method and a combination index method.

Abstract: The disclosure relates to a zearalenone-deoxynivalenol dual-channel immunoquantitative test strip and belongs to the technical field of immunoassay rapid detection. The disclosure prepares fluorescent probes by labeling with fluorescent microspheres, including a fluorescent microsphere-zearalenone monoclonal antibody, a fluorescent microsphere-deoxynivalenol monoclonal antibody and a fluorescent microsphere-goat anti-mouse second antibody. A zearalenone artificial antigen, a fluorescent microsphere artificial antigen and a goat anti-mouse second antibody are respectively sprayed on a nitrocellulose membrane to serve as a detection line T1, a detection line T2 and a quality control line C to prepare the immunochromatographic test strip; a competitive immunoassay method is adopted, and zearalenone and deoxynivalenol in samples are quantitatively analyzed at the same time by reading fluorescence values of the detection lines on a fluorescence immunoanalyzer.

Abstract: A method, computer system, and a computer program product for detecting a session status based on a cookie associated with the session is provided. The present invention may include receiving an access request to a specified location associated with a server computer. The present invention may also include determining that the received access request has the cookie corresponding with the specified location. The present invention may also include receiving a last refresh time from the cookie. The present invention may then include determining the session status based on the retrieved last refresh time, a current request time, a refresh interval, and an overdue value.

Abstract: An example operation may include one or more of identifying partitions associated with a blockchain, identifying a new transaction to be committed to the blockchain, determining a partition code assigned to the new transaction, and committing the new transaction to one of the partitions associated with the partition code.

Abstract: Embodiments of the invention are directed to a computer-implemented method that includes determining, by a controller of a messaging system, a cognitive context of a message sender. The method also includes configuring at least one property of a message based on the determined cognitive context. The message is transmitted by the message sender to a first receiver. The method also includes transmitting the message to a second receiver based on the at least one configured property.

Abstract: The present disclosure belongs to the technical field of analytical chemistry, in particular to synthesis and application of a nanomaterial for removal of patulin (Pat). The present disclosure adopts 2-Oxin as a substitute template, AM as a functional monomer, and synthetic Fe3O4@SiO2@CS-GO magnetic nanoparticles as a carrier, for preparing a magnetic MIP specific for Pat adsorption by surface imprinting. The addition of Fe3O4 makes the finally prepared molecular imprinted adsorbent material magnetic, thereby facilitating separation of a material from a matrix, eliminating complicated operation steps such as filtration and centrifugation, and facilitating recovery of materials.

Abstract: An example operation may include one or more of identifying partitions associated with a blockchain, identifying a new transaction to be committed to the blockchain, determining a partition code assigned to the new transaction, and committing the new transaction to one of the partitions associated with the partition code.

Abstract: A method, computer system, and a computer program product for detecting a session status based on a cookie associated with the session is provided. The present invention may include receiving an access request to a specified location associated with a server computer. The present invention may also include determining that the received access request has the cookie corresponding with the specified location. The present invention may also include receiving a last refresh time from the cookie. The present invention may then include determining the session status based on the retrieved last refresh time, a current request time, a refresh interval, and an overdue value.

Abstract: The present invention relates to the field of automobile parts, and more particularly to an automobile accessory mounting support having: a body including a base and a tubular structure for housing the accessory, wherein one end of the tubular structure is fixed to the base and the other end is provided with one or more snapping members; and a cover for cooperating with the body, which is provided with a snapping structure for cooperating with the snapping members.

Abstract: A method, computer system, and a computer program product for detecting a session status based on a cookie associated with the session is provided. The present invention may include receiving an access request to a specified location associated with a server computer. The present invention may also include determining that the received access request has the cookie corresponding with the specified location. The present invention may also include receiving a last refresh time from the cookie. The present invention may then include determining the session status based on the retrieved last refresh time, a current request time, a refresh interval, and an overdue value.