Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions and methods that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells.

Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions and methods that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. For example, disclosed herein is a system for inducing targeted disruption of endogenous genes in a eukaryotic cell, the system comprising: an extracellular Cas9/RNA complex; cell-free and a buffer; wherein the extracellular Cas9/RNA complex comprises: a recombinant Cas9; and a guide RNA having a CRISPR RNA (crRNA) and a transactivating crRNA (tracrRNA); wherein the extracellular Cas9/RNA complex is disposed in the cell-free buffer and wherein the extracellular Cas9/RNA complex is complexed prior to being introduced into the eukaryotic cell; and wherein the Cas9/RNA complex functions as an endonuclease that induces targeted disruption of the target DNA upon introduction into the eukaryotic cell.

Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions and methods that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells.

Abstract: Proposed is a preparation method for single-layered MXenes capable of exfoliating a single layer of MXenes from multi-layered MXenes with a high yield. One embodiment of the present disclosure provides a preparation method for single-layer MXenes, the method includes exfoliating a single layer of MXenes from multi-layered Mxenes with a Couette-Taylor reactor (CT reactor), inducing a regular flow.

Abstract: The present inventive concept relates to a non-face-to-face health status measurement system and method through a camera-based vital sign data extraction and an electronic questionnaire, providing a system and a method for monitoring various infectious diseases and enabling rapid response to the occurrence of diseases and infectious diseases by measuring a health status of a user in non-face-to-face through a vital sign data including heart rate, respiration rate, oxygen saturation, etc. extracted by using color information of a face image taken with a camera and results of an electronic questionnaire performed online.

Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. The composition may comprise a single-chain guide RNA (sgRNA) and a Streptococcus pyogenes Cas9 protein. In some embodiments, the sgRNA and the Cas9 protein may be present in a molar ratio ranging from 29:14.0 to 29:1.4.

Abstract: A battery state estimating apparatus according to an embodiment of the present disclosure includes a measuring unit configured to measure a charging current, and voltage and temperature of a battery in a constant current charging process and a constant voltage charging process of the battery; and a control unit configured to judge whether an abnormal behavior occurs in the battery according to a behavior of at least one of the charging current, the voltage of the battery and the temperature of the battery in at least one of the constant current charging process and the constant voltage charging process, and determine whether lithium precipitation occurs in the battery based on the judged abnormal behavior of the battery.

Abstract: A particle heat exchanger comprising: a housing including an inlet located at the top of the housing, and an outlet located below the inlet, the housing configured to enclose a flow of heat transfer particles which flows downwardly from the inlet to the outlet within the housing; at least one heat transfer tube enclosed in the housing and in contact with the flow of heat transfer particles therein, each heat transfer tube extending substantially parallel to an axis extending between the inlet and outlet of the housing; and at least one divider located between the inlet and outlet of the housing, the at least one heat transfer tube extending through each divider, each divider including at least one opening configured to form at least one flow constriction in the flow of heat transfer particles between the inlet and outlet of the housing.

Abstract: A storage device includes a controller and nonvolatile memories. The controller receives write commands having virtual stream identifiers (IDs), receives discard commands having the virtual stream IDs, and determines a lifetime of write data to which each of the virtual stream IDs is assigned. The nonvolatile memories are accessed by the controller depending on physical stream IDs. The controller maps the virtual stream IDs and the physical stream IDs based on the lifetime of the write data.

Abstract: The present invention provides a polyamic acid aqueous solution composition capable of polymerizing polyamic acid in water rather than in an organic solvent, as well as achieving a high imidization rate during low-temperature curing.

Abstract: A jelly roll type electrode assembly a cathode sheet, an anode sheet, and a separator sheet interposed between the cathode sheet and the anode sheet. A separator sheet part facing one end of the cathode sheet located an inner side of the jelly roll type electrode assembly includes a first surface facing the anode sheet and a second surface facing the cathode sheet. A friction coefficient between the first separator sheet and the anode sheet is smaller than a friction coefficient between the second surface of the separator sheet and the cathode sheet.

Abstract: The embodiments may provide a steering control device of a vehicle equipped with a steer-by-wire steering system and a rear wheel steering system. A steering control device may include a sensor configured to acquire information on the vehicle, and a controller configured to, in response to a determination of a failure of the steer-by-wire steering system, determine a target yaw rate according to a target steering angle based on the information acquired from the sensor, and generate and output a differential braking control signal and a rear wheel steering control signal to follow the target yaw rate.

Abstract: A solid particle solar receiver comprising: a housing which includes at least one opening for receiving concentrated solar irradiance; and an inclined receiver surface about and along which particles fall downwardly from a particle inlet, the receiver surface being located in the housing in a position through which concentrated solar irradiance can incident through the at least one opening, wherein the receiver surface comprises at least two particle falling stages, each stage separated by at least one particle retention formation configured to receive, accumulate and progressively discharging particles into a subsequent stage. and wherein the receiver surface is configured with a frustoconical shaped curve.

Abstract: The present application provides a polyamic acid aqueous solution composition prepared of a polyimide, wherein the composition enables the polymerization of hydrophobic-based monomers into a polyamic acid in water and is improved in transparency, eco-friendliness, storage stability, and the like when cured.

Abstract: A host, a storage system, and an operating method of the host are provided. The host includes a host memory configured to store a tree structure including a leaf node and an index node, an index management module configured to manage an index based on the tree structure and generate a first log corresponding to the leaf node based on a first update request corresponding to a first key-value entry included in the leaf node, and a device driver configured to generate a first write command corresponding to the first log and transmit the generated first write command to a key-value storage device, so as to store the first log in the key-value storage device. The index management module is configured to generate a first new key-value entry, the first new-key value entry including a first value updated based on the first update request, as the first log.

Abstract: Provided is a blade stabilization apparatus for a relief damper, and more specifically, to a blade stabilization apparatus for a relief damper which can stably maintain an opening angle of a damper blade at any angle when the damper opens or closes after the center of gravity is always aligned with a centerline of a rotating shaft in a perpendicular direction to the damper blade.

Abstract: Disclosed is a reconfigurable metasurface antenna. The reconfigurable metasurface antenna includes: a power feeding unit receiving and propagating electromagnetic waves in the form of a concentric wave; and a variable metasurface unit positioned at an upper part of the power feeding unit, having a plurality of unit radiators, and radiating the electromagnetic waves transmitted from the power feeding unit according to a combination of activated unit radiators among the plurality of unit radiators to have different phase and polarization components.

Abstract: Provided is a blade stabilization apparatus for a relief damper, and more specifically, to a blade stabilization apparatus for a relief damper which can stably maintain an opening angle of a damper blade at any angle when the damper opens or closes after the center of gravity is always aligned with a centerline of a rotating shaft in a perpendicular direction to the damper blade.

Abstract: The present invention relates to a cytosine or adenine deaminase in an isolated form or a variant thereof, a non-toxic full-length cytosine deaminase or a variant thereof, a fusion protein comprising same, a composition for base editing, and a method for editing a base by using same.

Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for Cas9/RNA complexes that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. The Cas9/RNA complex may comprise a recombinant Cas9 protein including a nuclear localization signal (NLS) and a guide RNA including a crRNA and a tracrRNA. The Cas9/RNA complex may be a combination of the recombinant Cas9 protein and the guide RNA. The guide RNA may be transcribed in vitro or synthesized chemically. The target endogenous nucleic acid sequence may include a portion complementary to the crRNA of the guide RNA.