Abstract: The present invention discloses a CRISPR/LpCas9 gene editing system and application thereof, the CRISPR/LpCas9 gene editing system includes a complex of LpCas9 protein and sgRNA, which can accurately locate a target DNA sequence and cleave DNA double strands. The LpCas9 protein has an amino acid sequence shown in SEQ ID NO:1; and the sgRNA has a nucleotide sequence shown in SEQ ID NO:2, or a modified sgRNA sequence based on SEQ ID NO: 2. The present invention can effectively solve the problems of heterologous codon bias and cytotoxicity in the application of SpCas9 in Lactobacillus paracasei. The gene editing is performed in cells or in vitro by introducing artificially designed CRISPR RNA and a repair template, which solves the problem of low electroporation efficiency caused by the strains' characteristics or relatively large carried plasmids, and has broad application prospects in the field of gene editing.

Abstract: Aspects of the present disclosure are directed to methods, systems, and computer program products for using dynamic-link library based on memory size. In the method, a request for calling a first function in a Dynamic-link library (DLL) at runtime is received first. A size of a memory allocated to the DLL is then determined. Then call relationship of functions in the DLL is obtained. At last, functions related to the first function in the DLL are loaded into the memory allocated to the DLL based on the size of the memory allocated to the DLL and call relationship of functions in the DLL.

Abstract: A computer-implemented method for termination of programs associated with different addressing modes includes receiving a call to an external interface to execute a target callee program from a caller program executing in a primary runtime environment. The external interface allocates an interoperability term area (ITA) in a primary runtime environment. The ITA is accessible by the primary runtime environment and a secondary runtime environment. The external interface executes the target callee program in the secondary runtime environment. The target callee program sets a termination reason parameter in the ITA. In response to the target callee program setting the termination reason parameter, a termination action in the primary runtime environment is performed. Various other methods, systems, and computer-readable media are also disclosed.

Abstract: Tapered stator designs are engineered in a positive displacement motor (PDM) power section to relieve stator stress concentrations at the lower (downhole) end of the power section in the presence of rotor tilt. A contoured stress relief (i.e. a taper) is provided in the stator to compensate for rotor tilt, where the taper is preferably more aggressive at the lower end of the stator near the bit.

Abstract: The invention relates to a catalytic article comprising a substrate having an inlet and an outlet; a first coating comprising a blend of: (1) platinum on a support, and (2) a first SCR catalyst comprising a Cu- and Mn-exchanged molecular sieve; and a second coating comprising a second SCR catalyst; wherein the support comprises at least one of a zeolite or a SiO2-Al2O3 mixed oxide. The platinum may be fixed on the support in solution.

Abstract: Method of constructing a vault of a large storage tank for liquefied natural gas by first modeling the vault with a 3-D modeling software application, then partially building the vault with a framework and a first set of covering panels fixed on the framework where the panels do not touch each other, but leave a number of gaps between them, measuring the dimensions of the actual gaps between the panels using a 3-D scanner, producing a second set of panels according to the scanned dimensional data, and finally filled the gaps between the first set of panels with the second set of panels, which are much smaller than the first set of panels, making the building process earlier and more accurate, which are difficult issues in building large tanks for liquefied natural gas.

Abstract: A computing device includes: a memory storing an audio sequence and a reference attribute associated with playback of the audio sequence; a speaker; a microphone; an audio enclosure supporting the speaker and the microphone; a processor configured to: simultaneously (i) control the speaker to play the audio sequence, and (ii) control the microphone to capture a test recording corresponding to playback of the audio sequence; based on a comparison of the reference attribute to a test attribute associated with the test recording, detect occlusion of the audio enclosure; and in response to detecting occlusion of the audio enclosure, generate a notification message.

Abstract: Techniques for signal handling between programs associated with different addressing modes in a computer system are described herein. An aspect includes, based on a signal occurring during execution of a first program in a first runtime environment, wherein the first program and the first runtime environment are associated with a first addressing mode, invoking a first signal exit routine associated with the first addressing mode. Another aspect includes allocating a signal information area (SIA) by the first signal exit routine. Another aspect includes calling a second signal exit routine associated with a second addressing mode that is different from the first addressing mode with an address of the SIA. Another aspect includes allocating a mirror SIA by the second signal exit routine. Another aspect includes handling the signal, and resuming execution based on the handling of the signal.

Abstract: Embodiments of the disclosure provide a data write-in method and apparatus. The data write-in method includes: selecting a target replica server from a plurality of replica servers managed by a metadata server according to remaining capacity of the plurality of replica servers; selecting a write-in disk in the target replica server according to remaining capacity and load of disks managed by the target replica server; and storing write-in data into the write-in disk through the target replica server.

Abstract: A contactor including a pedestal. The pedestal is of a step structure having notches in two ends. The step structure includes a first step and a second step from bottom to top and is covered with a cover body having a matched shape. A part, which protrudes out of the second step, at each of two ends of the first step, is provided with a first mounting groove in which a control terminal and a power terminal that are spaced apart from each other are mounted, and the cover body is provided with a first wiring port corresponding to the control terminal and a second wiring port corresponding to the power terminal. Each of two ends of the second step is provided with a second mounting groove in which an auxiliary terminal is mounted, and the cover body is provided with a third wiring port which corresponds to the auxiliary terminal mounted in the second mounting groove. The contactor has a simple structure, and wiring areas can be distinguished.

Abstract: Unlaminated bearings (or Torque Transfer Elements, or TTEs) are disposed to slide and displace within pockets (or “housing cavity receptacles”) provided in the internal periphery of a housing in which an articulating shaft is received. As the shaft “tilts” about its untilted axial centerline during misaligned rotation, convex curved bearing surfaces on shaft pins slidably rotate against corresponding concave curved bearings surfaces on the T as received in the housing cavity receptacles. Further, substantially flat surfaces on the TTEs are disposed to slidably displace against corresponding bearing surfaces on the housing cavity receptacles as the shaft tilts and the convex curved bearing surfaces on the shaft pins slidably rotate against the concave curved bearing surfaces on the TTEs. The sliding displacement of TTEs with respect to the housing cavity receptacles during articulated rotation is in a direction generally parallel to the shaft's untilted axial centerline.

Abstract: A catalyst article including a substrate with an inlet side and an outlet side, a first zone and a second zone, where the first zone includes a passive NOx adsorber (PNA), and an ammonia slip catalyst (ASC) comprising a platinum group metal on a support and a first SCR catalyst; where the second zone includes a catalyst selected from the group consisting of a diesel oxidation catalyst (DOC) and a diesel exotherm catalyst (DEC); and where the first zone is located upstream of the second zone. The first zone may include a bottom layer with a blend of: (1) the platinum group metal on a support and (2) the first SCR catalyst; and a top layer with a second SCR catalyst, the top layer located over the bottom layer.

Abstract: An example operation may include one or more of generating an initial seed and allocating one or more authorized bits of the initial seed to a plurality of blocks in a distributed ledger, storing the initial seed and an identification of which authorized bits of the initial seed are allocated to each block of the distributed ledger, receiving a final seed value that is partially generated by each of a plurality of nodes configured to access the distributed ledger based on authorized bits of respective blocks updated by each respective node, and generating a random sequence value based on the final seed value and storing the random sequence value in a block of the distributed ledger.

Abstract: A catalytic article for treating an exhaust gas stream containing particulate matter, hydrocarbons, CO, and ammonia, the article may include: (a) a substrate having an inlet end and an outlet end defining an axial length; (b) a first catalyst coating including: 1) a platinum group metal distributed on a molecular sieve, and 2) a base metal distributed on a molecular sieve; and (c) a second catalyst coating including: 1) a platinum group metal distributed on a molecular sieve, and 2) a base metal distributed on a molecular sieve.

Abstract: Systems, methods, and computer-readable media are described for interoperability between programs associated with different addressing modes. A caller program associated with a first addressing mode (e.g., a 32-bit program or a 64-bit program) that is executing within a parent runtime environment makes a call to an external interface to execute a target callee program that is associated with a second addressing mode different from the first addressing mode. The target callee program is then executed in a pre-initialized secondary runtime environment that executes on the same task or thread as the parent runtime environment.

Abstract: The present disclosure relates to graphene. In particular, the present disclosure relates to a method for continuously preparing thermally conductive graphene films. A graphite oxide containing 40-60 wt % of moisture is directly stripped at a high temperature; and then, procedures such as dispersion, defoaming, coating, stripping, trimming, and reduction are performed to prepare thermally conductive graphene films with high thermal conductivity coefficient and strong electromagnetic shielding effectiveness. In the method, because of directly stripping the graphite oxide containing 40-60 wt % of moisture at a high temperature, the procedure of drying the graphite oxide is omitted, achieving low energy consumption and low manufacturing costs. Compared with preparing slurry by directly dispersing the graphite oxide, the concentration of the slurry after high temperature stripping is higher, and can reach 3-20 wt %.

Abstract: The present invention relates to a novel therapeutic drug for a neurodegenerative disease and an application thereof. Provided is a novel compound of formula (I). The compound can effectively facilitate the proliferation of neural stem cells in both in vitro and in vivo experiments and can be used as a treatment approach for facilitating neuroregeneration to fight against cognitive decline associated with aging or a neurodegenerative disease.