Abstract: Column for liquid chromatography contains a glass tube for sorbent packing closed with simple column seals and inserted into a jacket made of metal or plastic material. The jacket transfers axial forces exerted on the column seals by liquid pressure action by means of sockets. It secures the tightness of the column, enables the connection of the column to a chromatographic system, and has a protective function at the same time. The columns can be coupled in series by means of doubled sockets.

Abstract: A storage container of samples for analysis is disclosed, having a cylindrical tube and two fittings adapted for connection with a syringe, mutual connection of containers in series, or closing and manufactured from a suitable plastic or glass. A column of sorbent is closed at both ends with a porous partition, screen, paper filter, or a layer of silicate or glass wool. The size of sorbent particles is 20-150 um (micrometers). The content of the storage container is protected during storage and/or transportation by closures from both sides. The sorbents which are packed into the storage container are selected from silica gel or organic copolymeric carriers of specific or nonspecific functional groups, which are purposefully chosen.

Abstract: The invention pertains to a method for the production of bead dextran materials for gel chromatography.The subject of the invention is a method for the production of bead dextran materials for gel chromatography, wherein dextran is, before or during the suspension process or in both stages, subjected to reduction in an alkaline solution, advantageously by the action of sodium borohydride, whereas the density of crosslinking is controlled above all by variation of the concentration of the initial dextran solution and the resulting gel may be optionally subjected to the additional crosslinking in a separate subsequent operation.The procedure according to the invention provides the product of higher quality with a lower content of carboxylic groups and substantially facilitates the preparation of gels with a very high and a very low crosslink density.

Abstract: A two-step process of immobilization of biologically efficient compounds is disclosed, in which the biologically efficient compounds are bonded from an aqueous solution by a hydrolytically stable linkage to a macroporous polymeric carrier containing reactive functional groups. In the first step, a hydrophobic adsorption occurs of the biologically efficient compounds on a macroporous matrix of the carrier, and an increase of their concentration on the solid surface. In the second step, the reaction takes place with the reactive functional groups of the carrier, and a covalent linkage is formed. The hydrophobic adsorption of compounds determined for immobilization is achieved by the addition of inorganic salts in a concentration from 0.5 to 3.0 mol/l. The invention can be utilized in the preparation of enzymatic catalysts for transformations of compounds and in the preparation of specific sorbents for both analytical and preparative affinity chromatography.

Abstract: The invention relates to selectively acting polymeric sorbents or saccharides, glycoproteins and saccharide containing polymers. In accordance with the invention, lectins are covalently bonded on vehicles selected from among, oxidized glycosylated hydroxyalkylacrylates or hydroxyalkylmethacrylates, and polyvinyl alcohol. These vehicles are comparable to known materials based on polysaccharides with bonded-on lectins, especially as far as the absorption power of the sorbent is concerned. The preferred process for preparing such sorbents is substantially easier than the hitherto used ones since it does not require specific measures to be taken in the work with highly toxic activating agents, and enables a simple control of conversion degree of the bonding reaction while achieving a relatively high reproducibility. Apart from this, unoxidized saccharide molecules bonded on the vehicle make it hydrophilic, or at least raise its original hydrophility.

Abstract: The invention pertains to hydrophilic macroporous three dimensional copolymers of hydroxyalkyl methacrylates or hydroxyalkyl acrylates (C.sub.1 -C.sub.4 hydroxyalkyl) with crosslinking monomers which contain covalently attached saccharides via an O-glycosidic bond. The invention further pertains to the method of manufacturing of these copolymers by the direct reaction of the basic three dimensional copolymer containing hydroxyl groups with saccharides or their derivatives in an inert organic solvent under catalysis of HCl, BF.sub.3, or their mixtures at 20.degree.-100.degree. C. Saccharides and their derivatives are selected from the group comprising monosaccharides, oligosaccharides, deoxy sugars, amino saccharides, acylated saccharides, ether or halogen derivatives of monosaccharides and oligosaccharides, and the like.

Abstract: The invention pertains to hydrophilic macroporous threedimensional copolymers of hydroxyalkyl methacrylates or hydroxyalkyl acrylates (C.sub.1 -C.sub.4 hydroxyalkyl) with crosslinking monomers which contain covalently attached saccharides via an O-glycosidic bond. The invention further pertains to the method of manufacturing of these copolymers by the direct reaction of the basic three dimensional copolymer containing hydroxyl groups with saccharides or their derivatives in an inert organic solvent under catalysis of HCl, BF.sub.3, or their mixtures at 20.degree.-100.degree. C. Saccharides and their derivatives are selected from the group comprising monosaccharides, oligosaccharides, deoxy sugars, amino saccharides, acylated saccharides, ether or halogen derivatives of monosaccharides and oligosaccharides, and the like.

Abstract: This invention relates to a method for preparing globular, hydrophilic copolymers which have reticulated internal structures and are suitable for gas and gel chromatography. The method comprises polymerization by suspension polymerization methods of hydrophilic and hydrophilic monomers in aqueous medium in the presence of a component which prevents transfer of the hydrophilic monomer into the aqueous medium and the solubility of the aqueous medium in the monomeric phase.

Abstract: This invention relates to a method for preparing globular, hydrophilic copolymers which have reticulated internal structures and are suitable for gas and gel chromatography. The method comprises polymerization by suspension polymerization methods of hydrophilic and hydrophilic monomers in aqueous medium in the presence of a component which prevents transfer of the hydrophilic monomer into the aqueous medium and the solubility of the aqueous medium in the monomeric phase.

Abstract: The invention relates to specific sorbents based on hydrophylic polymeric gels, preferably of a macroporous character, which carry covalently bonded D-aminoacid or peptide which contain D-aminoacid units. The basic carrier prepared by copolymerization of hydroxyalkyl esters or hydroxyalkylamides of acrylic and methacrylic acid with crosslinking acrylate or methacrylate comonomers are modified by the reaction with diamines, aminoacids or dicarboxylic acids and the resulting carboxyterminal or aminoterminal groups are condensed with D-analogs of aminoacids or peptides. The peptide containing D-aminoacids can be also stepwise synthetized on the surface of the carrier. The specific sorbents are further used as insolubilized inhibitors in the affinity chromatography of proteolytic enzymes. They do not undergo an irreversible inactivation during repeated application and are suitable for technological purposes because of their mechanical and hydrolytical stability.

Abstract: The invention relates to preparation of synthetic macroporous ion-exchanaging materials by a ternary copolymerization of hydrophilic monomers containing non-ionogenous groups with monomers containing ionogenous groups and cross-linking monomers in an aqueous dispersion in the presence of inert components and suspension stabilizers. Hydroxyalkyl, oligoglycol and polyglycol esters and N-substituted or unsubstituted amides of acrylic and methacrylic acid are used as the hydrophilic monomers; compounds CH.sub.2 .dbd.C(R)COX, where R = H or CH.sub.3, X = --OR.sub.1 N(R.sub.2)R.sub.3, --OR.sub.1 SO.sub.3 H, --NHR.sub.1 N(R.sub.2)R.sub.3 or --HNR.sub.1 SO.sub.3 H, R.sub.1 = alkylene and R.sub.2 and R.sub.

Abstract: The invention relates to macroporous polymeric sorbents prepared by ternary copolymerization of polar monomers, namely hydroxyalkyl acrylates and methacrylates (hydroxyalkyl C.sub.1-6), in the amount of 1 - 70 wt.%, with 30 - 90 wt.% of nonpolar comonomers, namely alkyl acrylates and methacrylates (alkyl C.sub.1-18), and 20 - 80 wt.% of crosslinking agents, namely alkylene diacrylates and dimethacrylates (alkylene C.sub.1-6). The sorbents were prepared by the suspension copolymerization in the presence of inert organic solvents and surfactants in the single step without further chemical modification and their polarity was controlled in a broad region. They are especially suitable for gas-chromatographic separation of organic compounds, as hydrocarbons, alcohols, organic acids and nitrogen-containing heterocyclic compounds. Their preparation in one step is more simple and economical than modification of the polymeric matrix by polymeranalogous reactions which has been used for this purpose until present time.

Abstract: An acrylic polymer gel suitable for use as a carrier in the controlled synthesis of polypeptides is provided. The polymer comprises a diazotized terpolymer of the condensation product of a monomer selected from the group consisting of hydroxyalkyl acrylates and hydroxyalkyl methacrylates, a polyfunctional cross-linking monomer selected from the group consisting of alkylene diacrylates, alkylene dimethacrylates, polyacrylates and polymethacrylates of polyalcohols, alkylene bisacrylamides and divinylbenzene, and an aromatic ring-containing compound selected from the group consisting of aromatic acids and their anhydrides and chlorides, aromatic isocyanates, halogenophenylsilanes, and aromatic epoxy compounds. The condensate has its aromatic rings chlormethylated. The first monomer and the polyfunctional cross-linking monomer are copolymerized and the resultant copolymer is condensed with said compounds which contain an aromatic ring.

Abstract: The invention relates to a method for preparation of ion exchangers from hydrophilic anion-exchanging gels based on acrylate or methacrylate copolymers prepared by copolymerization of amino-group-containing monomers, as aminoalkyl acrylates and methacrylates, aminoalkylacrylamides and aminoalkylmethacrylamides or on gels prepared by chemical bonding of amine groups to the hydrophilic matrix of acrylate or methacrylate copolymers. The method consists in alkylation or hydroxyalkylation of amine groups by alkylating agents, as alkyl halogenides, alkyl p-toluenesulfonates, alkyl sulfates, epoxides, etc. Alkylation with alkyldiaze compounds was used for transformation of the undesired carboxylic groups of the ion exchangers. Another modification consists in the reaction of halogenated acrylate or methacrylate gels with polyamines or oligomeric polyethyleneimines.

Abstract: The invention relates to a method for preparation of hydrophilic anion exchangers from gels based on crosslinked copolymers of hydroxyalkyl acrylates and methacrylates, oligo- and polyglycol monoacrylates and monomethacrylates, hydroxyalkylacrylamides or hydroxyalkylmethacrylamides with crosslinking comonomers containing 2 or more acrylcyl or methacryloyl groups in the molecule, as alkylene, oligo- and polyglycol diacrylates and dimethacrylates or divinylbenzene, which gels may have the homogeneous, heterogeneous or macroporous character.

Abstract: The invention relates to a method for preparation of hydrophilic macroporous ion exchangers of the amphoteric character which are suitable above all for isolation and separation of biological materials. The preparation consists in copolymerization of anionogenous monomers, as sulfoalkyl acrylates and methacrylates, sulfoalkylacrylamides, sulfoalkylmethacrylamides, acrylic and methacrylic acid, with cationogenous monomers, as aminoalkyl acrylates and methacrylates, aminoalkylacrylamides, aminoalkylmethacrylamides and their quaternary ammonium derivatives, and with crosslinking monomers, as alkylene or oligo and polyglycol diacrylates and dimethacrylates, bisacrylamides, bismethacrylamides and divlnylbenzene, in the water dispersion system containing inert organic compounds, as alcohols, acids, amines or nitriles.

Abstract: There is disclosed a composition of matter comprising a polymer containing aromatically bound mercuric ions having the formula ##STR1## wherein R is H or CH.sub.3, Y is -- CO(OCH.sub.2.CH.sub.2).sub.x -- O --, wherein x = 0-20, or -- CONH --, Ar is a bifunctional aromatic group, Z = H or NH--R.sup.2 wherein R.sup.2 is acyl, carboxy C -- to -- alkyl or a sulfonyl group, and R.sup.1 is the chain of homopolymer of the formula ##STR2## or the chain of a copolymer selected from the group consisting of methacrylic or acrylic esters of polyfunctional alcohols, methacrylamides or acrylamides, monomers containing one or more acrylic or methacrylic acid groups and monomers of the acrylic or methacrylic series, method of preparing such polymers, carriers produced on the basis of such polymers and method of extracting sulfur-containing compounds from mixtures containing the same utilizing the polymers of the invention.

Abstract: There is disclosed a method for the production of carriers for biologically active compounds comprising forming a bond by admixing (a) a three dimensional water insoluble diazotizable polymer operative to function as a primary carrier and (b) a water soluble linear polymer having ligands operative to bind said biologically active compounds and operative to function as a secondary carrier, whereby the union of (a) and (b) is operative to serve as a carrier for biologically active compounds and carriers produced in accordance therewith.

Abstract: An acrylic polymer suitable for use as carrier in the controlled synthesis of polypeptides is provided. The polymer comprises the condensation product of a monomer selected from the group consisting of hydroxyalkyl acrylates and hydroxyalkyl methacrylates, a polyfunctional cross-linking monomer selected from the group consisting of alkylene diacrylates, alkylene dimethacrylates, polyacrylates and polymethacrylates of polyalochols, alkylene bisacrylamides and divinylbenzene, and an aromatic ring-containing compound selected from the group consisting of aromatic acids and their anhydrides and chlorides, aromatic isocyanates, halogenophenylsilanes, and aromatic epoxy compounds. The condensate has its aromatic rings chlormethylated. The first monomer and the polyfunctional cross-linking monomer are copolymerized and the resultant copolymer is condensed with said compounds which contain an aromatic ring.

Abstract: A polymeric material containing primary amino groups is prepared by copolymerizing an acylaminophenoxy acrylate or methacrylate with a hydroxyalkyl ester or an amide of acrylic or methacrylic acid, and treating the resulting copolymeric material with a hydrolytic agent to convert the acylamino groups into primary amino groups. Such monomeric materials may also be copolymerized with a crosslinking agent comprising an ester or an amide containing two or more acryloyl or methacryloyl groups.