Abstract: A microfluidic device is for processing and aliquoting a sample liquid. The microfluidic device has a dividing chamber for receiving a starting volume of the sample liquid. The dividing chamber has a plurality of cavities for receiving sub-volumes of the sample liquid, the sub-volumes being usable for analytical reactions. The microfluidic device also has a microfluidic network for using the dividing chamber in a fluid-mechanical manner and at least one pump device for pumping fluids within the device. The at least one pump device and the microfluidic network are configured to pump the sample liquid, as a first phase, and a sealing liquid, as a second phase, through the microfluidic network and into the dividing chamber in order to seal the sub-volumes of the sample liquid in the cavities using the sealing liquid.

Abstract: A microfluidic device is for processing and aliquoting a sample liquid. The microfluidic device has a dividing chamber for receiving a starting volume of the sample liquid. The dividing chamber has a plurality of cavities for receiving sub-volumes of the sample liquid, the sub-volumes being usable for analytical reactions. The microfluidic device also has a microfluidic network for using the dividing chamber in a fluid-mechanical manner and at least one pump device for pumping fluids within the device. The at least one pump device and the microfluidic network are configured to pump the sample liquid, as a first phase, and a sealing liquid, as a second phase, through the microfluidic network and into the dividing chamber in order to seal the sub-volumes of the sample liquid in the cavities using the sealing liquid.

Abstract: A method for detecting characteristic cells in a sample includes performing a first lysis of a sample to yield a first lysate wherein nuclei of the cells of the sample remain intact. Freely available RNA in the first lysate is extracted and classified to ascertain the amounts of various types of RNA in the first lysate. A second lysis operation on the previously lysed sample is performed to yield a second lysate wherein the cell nuclei of the cells in the sample are destroyed. DNA freely available in the second lysate is extracted and a total cell count of cells in the provided sample is calculated on the basis of the amount of extracted DNA. The number of cells having individual cell markers in the provided sample is calculated using the ascertained amounts of various types of RNA and the calculated total cell count.

Abstract: A fluidic network for carrying out, in parallel, a plurality of analyses of biological samples is disclosed. The network has a flow cell array with a plurality of reaction chambers. The reaction chambers each have a first channel connection and a second channel connection. The first channel connections are connected to a first supply channel and the second channel connections are connected to a second supply channel. The first supply channel and the second channel connection are interconnected by a circulation line. At least one component is connected to the circulation line so that component test reagents can be introduced into the reaction chambers of the flow cell array.

Abstract: A microfluidic sequencing device for multiplying and separating molecular chains, and which is designed to receive biochemical material, includes at least one supply opening for supplying biochemical material into the sequencing device. The sequencing device additionally has at least one microfluidic separation unit, which has at least one separation channel with at least one amplification cavity for multiplying molecular chains supplied via the supply opening as the biochemical material and with at least one separating unit which is connected or can be connected to the amplification cavity microfluidically and includes a multi-porous material. The separating unit is designed to separate nucleic acids from other macromolecular components and/or to separate nucleic acids. Furthermore, the sequencing device has at least one discharge opening for discharging nucleic acids separated in the separation unit as biochemical material out of the sequencing device.

Abstract: A process for detecting nucleus-containing cells in a sample liquid of a patient using a microfluidic device is disclosed. The process includes (i) providing a mixing signal to a mixing device, wherein the mixing signal causes mixing of the sample liquid with a lysis buffer in a mixing chamber of the microfluidic device in order to obtain a lysate, (ii) outputting an application signal which causes application of the lysate onto a carrier substrate of the microfluidic device in order to obtain a cell sediment and cell suspension of the lysate, and (iii) identifying the nucleus-containing cells from the cell sediment.

Abstract: A microfluidic dual cartridge includes a first microfluidic analysis device for processing sample material and a second microfluidic analysis device for processing sample material. The two analysis devices being interconnected at a connection point, which is configured to bring about a defined separation of the first microfluidic analysis device and the second microfluidic analysis device under the effect of a force.

Abstract: A method for trapping at least one nucleated cell using at least one electrode for a microfluidic device is disclosed. The method includes (i) outputting an application signal that causes a sample liquid comprising the at least one nucleated cell to be applied to a carrier substrate of the microfluidic device, and (ii) providing a current signal to an interface with the at least one electrode in order to generate, at or in a microcavity of the carrier substrate, an electric field configured to trap the at least one nucleated cell as a target cell in the microcavity.

Abstract: A microfluidic cartridge has an L-shaped base. The base is based on a rectangle and a corner region of the rectangle is missing, so that the microfluidic cartridge is positionable relative to a second microfluidic cartridge having the same base, such that a respective projection of the base of the microfluidic cartridge engages in the missing corner region of the base of the second microfluidic cartridge.

Abstract: The disclosure relates to a microfluidic analysis device and to a method for operating the microfluidic analysis device. The method comprises the following steps: providing a sample containing DNA, performing a PCR pre-amplification of the sample, dividing the sample into at least two reaction compartments, and performing at least one singleplex detection in each of the at least two reaction compartments. The singleplex detection is performed in each case by means of an isothermal amplification system.

Abstract: A method of sequencing a prepared DNA strand includes providing the prepared DNA strand with nucleotides of the types dATP, dCTP, dGTP and/or dTTP, wherein at least one of the types of the nucleotides comprises a predetermined magnetic label. The method further includes placing the prepared DNA strand within a measuring range of a sensor unit comprising an operatively connected magneto-optical transducer unit and an optical sensor and optically exciting the magneto-optical transducer unit. The method continues with acquiring at least one value indicative of a fluorescence signal of the magneto-optical transducer unit, and assigning the acquired value to the at least one type of the nucleotides comprising the predetermined magnetic label.

Abstract: A microfluidic device is for processing and aliquoting a sample liquid. The microfluidic device has a dividing chamber for receiving a starting volume of the sample liquid. The dividing chamber has a plurality of cavities for receiving sub-volumes of the sample liquid, the sub-volumes being usable for analytical reactions. The microfluidic device also has a microfluidic network for using the dividing chamber in a fluid-mechanical manner and at least one pump device for pumping fluids within the device. The at least one pump device and the microfluidic network are configured to pump the sample liquid, as a first phase, and a sealing liquid, as a second phase, through the microfluidic network and into the dividing chamber in order to seal the sub-volumes of the sample liquid in the cavities using the sealing liquid.

Abstract: A fluidic network for carrying out, in parallel, a plurality of analyses of biological samples is disclosed. The network has a flow cell array with a plurality of reaction chambers. The reaction chambers each have a first channel connection and a second channel connection. The first channel connections are connected to a first supply channel and the second channel connections are connected to a second supply channel. The first supply channel and the second channel connection are interconnected by a circulation line. At least one component is connected to the circulation line so that component test reagents can be introduced into the reaction chambers of the flow cell array.

Abstract: A method for detecting characteristic cells in a sample includes performing a first lysis of a sample to yield a first lysate wherein nuclei of the cells of the sample remain intact. Freely available RNA in the first lysate is extracted and classified to ascertain the amounts of various types of RNA in the first lysate. A second lysis operation on the previously lysed sample is performed to yield a second lysate wherein the cell nuclei of the cells in the sample are destroyed. DNA freely available in the second lysate is extracted and a total cell count of cells in the provided sample is calculated on the basis of the amount of extracted DNA. The number of cells having individual cell markers in the provided sample is calculated using the ascertained amounts of various types of RNA and the calculated total cell count.

Abstract: A sequencing device has at least one sequencing channel configured to fluidically connect a first gap with a second gap. The sequencing channel is formed as a cavity in the region of the first gap and is formed as a pore in the region of the second gap. The pore has a smaller cross section than the cavity.

Abstract: A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.

Abstract: A method for carrying out a process for an amplification of nucleic acids with sample nucleic acids and reference nucleic acids being amplified in separate reaction batches. Signals of the amplification are observed in real time. A number of amplification cycles and/or a duration of the amplification process are dynamically adjusted depending on the observed signals of the amplification.

Abstract: A device to electrochemically sequence DNA that includes a redox species includes at least one edge electrode, at least one stack of insulator material, and a pair of DNA translocation electrodes including a DNA translocation working electrode and a DNA translocation counter electrode, where the thickness of the at least one edge electrode is about 0.5 nanometers, and the thickness of the at least one stack of insulator material is about 10 nanometers. Methods of electrochemically sequencing a strand of DNA are also provided.

Abstract: A microfluidic sequencing device for multiplying and separating molecular chains, and which is designed to receive biochemical material, includes at least one supply opening for supplying biochemical material into the sequencing device. The sequencing device additionally has at least one microfluidic separation unit, which has at least one separation channel with at least one amplification cavity for multiplying molecular chains supplied via the supply opening as the biochemical material and with at least one separating unit which is connected or can be connected to the amplification cavity microfluidically and includes a multi-porous material. The separating unit is designed to separate nucleic acids from other macromolecular components and/or to separate nucleic acids. Furthermore, the sequencing device has at least one discharge opening for discharging nucleic acids separated in the separation unit as biochemical material out of the sequencing device.

Abstract: A method for diluting, mixing, and/or aliquoting two liquids using a microfluidic system with at least two pump chambers first includes filling at least one of the at least two pump chambers with a first liquid. The at least two pump chambers are connected to one another by at least one microfluidic channel. The method then includes pumping the first liquid through the channel. The at least one channel is configured such that at least part of the first liquid remains in the channel after the pumping. The method then includes determining the part of the first liquid that remains in the channel. The method then includes flushing the channel with a second liquid.