Abstract: A method and apparatus for light field microscopy, wherein the following method steps are performed: a) measuring an image data record of a sample using a light field arrangement; b) creating at least one partial data record from the image data record; c) reconstructing a three-dimensional object from the partial data record created in step b).

Abstract: Microscopy and a device for microscopy where with a light field arrangement comprising a multi-lens array and a camera having at least one camera sensor, image data sets which each contain at least one partial image of a sample are successively recorded. To increase a number of the image data sets recordable per unit time, a) measurement data only from a first number of pixels less than a total number of pixels of the at least one camera sensor are read out; b) measurement data only from a second number of pixels less than the total number of pixels or the first number of pixels read are processed further; and/or c) for some or all of the total number of pixels or the first number of pixels read, measurement data are processed further with a bit depth which is less than a maximum possible bit depth.

Abstract: As a result of the size of the detector elements thereof, optoelectronic detectors such as photoelectron multipliers comprising a light-entry region sealed by a protective disc can only be used with much outlay for recording an image of a diffraction-limited focus volume in a two-dimensional spatially resolved manner, even if the image is significantly magnified in relation to the focus volume. The novel detector is intended to enable the spatially resolved detection of point spread functions with little outlay and high accuracy. 2.2 For this purpose, a body made of glass or glass ceramics comprising an opening, in which one end of an optical fiber is arranged, is cemented to the cover disc in such a way that the end of the optical fiber faces the cover disc and the optical axis thereof intersects the light-entry region. Thus, the relative position of optical fiber and entry region can be provided permanently with high accuracy.

Abstract: As a result of the size of the detector elements thereof, optoelectronic detectors such as photoelectron multipliers comprising a light-entry region sealed by a protective disc can only be used with much outlay for recording an image of a diffraction-limited focus volume in a two-dimensional spatially resolved manner, even if the image is significantly magnified in relation to the focus volume. The novel detector is intended to enable the spatially resolved detection of point spread functions with little outlay and high accuracy. 2.2 For this purpose, a body made of glass or glass ceramics comprising an opening, in which one end of an optical fibre is arranged, is cemented to the cover disc in such a way that the end of the optical fibre faces the cover disc and the optical axis thereof intersects the light-entry region. Thus, the relative position of optical fibre and entry region can be provided permanently with high accuracy.

Abstract: A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.

Abstract: A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.

Abstract: An image processing device comprising an acquisition interface for acquiring recorded image data or recorded image signals and a graphics interface for a display device is constructed in such a way that a temporal sequence of recorded images can be acquired via the acquisition interface and an image data acquisition device connected to the latter and a temporal sequence of display images can be generated from the recorded image sequence, preferably with a smaller quantity of display images over the period of time in which the recorded image sequence is acquired. A display image of the display image sequence is generated from a partial sequence of at least two already acquired recorded images of the recorded image sequence, this partial sequence being associated with the display image of the display image sequence, and the display images can be sent to the display device via the graphics interface.

Abstract: A calibration device for managing a variety of performance tests and/or calibration tasks in a laser scanning microscope. The calibration device, which has focusing optics and a test structure arranged in the focal plane of the focusing optics, with structural elements detectable in reflected and/or transmitted light aligned to each other in a common mounting, can be switched into the microscope beam path in a laser scanning microscope, so that the pupil of the focusing optics coincides with the objective pupil of the laser scanning microscope or lies in a plane conjugated to it.

Abstract: In a method for correcting a control of an optical scanner (14) which has a beam deflecting element (31) for deflecting a beam of optical radiation and a drive unit (30, 30?) for moving the beam deflecting element (31), said drive unit deflecting a beam of optical radiation directed at the beam deflecting element (31) according to a predetermined target movement using at least one parameter and/or a transfer function, preferably optical, said parameter or transfer function being used to control or regulate the system. In a determination step at least one current value of a drive unit transfer function that reproduces the response of the drive unit (30, 30?) to a predetermined target movement or a change in a target movement is ascertained for at least one frequency, and in a correction step at least one parameter and/or the transfer function is corrected as a function of the current value of the drive unit transfer function.

Abstract: A diaphragm device with which individual wavelengths or ranges of wavelengths in the path of a beam of spectrally dispersed light can be suppressed. Such a diaphragm device comprises at least one array of diaphragms, wherein the individual diaphragms of the array are arranged in a definite relation to each other and may be coupled in the path of the beam and each diaphragm of the array in the coupled state is arranged in a given relation to an individual wavelength or a range of wavelengths.

Abstract: For a confocal scanning microscope (1) an optical zoom system (41) with linear scanning is provided, which not only makes a zoom function possible, in that a variable magnification of an image is possible, but rather which additionally produces a pupil image in the illuminating beam path (IB) [BS] and thereby makes a variable image length possible (distance between the original pupil (En.P) [EP] and the imaged/reproduced pupil (Ex.P) [AP]) so that axially varying objective pupil positions can thereby be compensated.

Abstract: In a method for correcting a control of an optical scanner (14) which has a beam deflecting element (31) for deflecting a beam of optical radiation and a drive unit (30, 30?) for moving the beam deflecting element (31), said drive unit deflecting a beam of optical radiation directed at the beam deflecting element (31) according to a predetermined target movement using at least one parameter and/or a transfer function, preferably optical, said parameter or transfer function being used to control or regulate the system. In a determination step at least one current value of a drive unit transfer function that reproduces the response of the drive unit (30, 30?) to a predetermined target movement or a change in a target movement is ascertained for at least one frequency, and in a correction step at least one parameter and/or the transfer function is corrected as a function of the current value of the drive unit transfer function.

Abstract: A spectral analytical unit for acting on a parallel light bundle having different wavelengths.

Abstract: A diaphragm device with which individual wavelengths or ranges of wavelengths in the path of a beam of spectrally dispersed light can be suppressed. Such a diaphragm device comprises at least one array of diaphragms, wherein the individual diaphragms of the array are arranged in a definite relation to each other and may be coupled in the path of the beam and each diaphragm of the array in the coupled state is arranged in a given relation to an individual wavelength or a range of wavelengths.

Abstract: Method for scanner control in at least one scan axis in a laser scanning microscope, the scan field being divided into partial area, a first image of at least one partial area produced by a forward scan being compared with a second image of the partial area produced by a back scan and a correction value for the scanner control determined from the deviation between the first and second image.

Abstract: A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.

Abstract: An image processing device comprising an acquisition interface for acquiring recorded image data or recorded image signals and a graphics interface for a display device is constructed in such a way that a temporal sequence of recorded images can be acquired via the acquisition interface and an image data acquisition device connected to the latter and a temporal sequence of display images can be generated from the recorded image sequence, preferably with a smaller quantity of display images over the period of time in which the recorded image sequence is acquired. A display image of the display image sequence is generated from a partial sequence of at least two already acquired recorded images of the recorded image sequence, this partial sequence being associated with the display image of the display image sequence, and the display images can be sent to the display device via the graphics interface.

Abstract: A diaphragm device with which individual wavelengths or ranges of wavelengths in the path of a beam of spectrally dispersed light can be suppressed. Such a diaphragm device comprises at least one array of diaphragms, wherein the individual diaphragms of the array are arranged in a definite relation to each other and may be coupled in the path of the beam and each diaphragm of the array in the coupled state is arranged in a given relation to an individual wavelength or a range of wavelengths.

Abstract: A correction device for an imaging optical arrangement exhibiting a light path (1), in particular for a microscope, that exhibits at least one plane-parallel transparent plate (9), which is held in a mounting plate in the image beam path (1) and is propelable around at least one axle in a tipping and/or a swiveling motion, in order in adjust a definite parallel misalignment of the beams in the image beam path (1) by a change in the tipping situation of the plate (9). A confocal microscope with such a correction device exhibits a confocal screen (4), which illustrates a specimen mark (10), whereby the plane-parallel plate (9) is placed in front of the detector unit (2) in the light path (1), in order to center the illustration of the aperture diaphragm on the detector unit.

Abstract: Method for correcting control of an optical scanner in a laser scanning microscope for imaging of a sample by scanning, the microscope guiding at least one beam path section of an illumination beam path of the microscope over the sample from an illumination device to the sample and/or an imaging beam path of the microscope from the sample to an acquisition device of the microscope in order to obtain an image of the sample, generating control signals corresponding to a predefined target movement using parameters and/or a transfer function of the scanner that are used for control and/or regulation and moving the at least one beam path section in response to the control signals, whereby an image of a reference sample having predefined structures imageable by the microscope is obtained by generating control signals corresponding to a predefined target test movement and moving the at least one beam path section in response to the control signals, thereby obtaining the image.