Patents by Inventor John C. Sanford
John C. Sanford has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7449449Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5? to 3? direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: GrantFiled: July 2, 2003Date of Patent: November 11, 2008Assignees: Duke University, Cornell UniversityInventors: Stephen A. Johnston, John C. Sanford
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Publication number: 20080138325Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5? to 3? direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: ApplicationFiled: November 19, 2007Publication date: June 12, 2008Inventors: Stephen A. JOHNSTON, John C. SANFORD
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Patent number: 7358234Abstract: The present invention relates to a process for inducing a protective immune response in a mammal against a virus, in which a construct including a promoter and a DNA sequence encoding a protein or peptide producing an immune response against the virus is introduced into muscle or skin of the mammal via a microprojectile. Sufficient amounts of the construct are administered so as to produce a protective immune response in the mammal.Type: GrantFiled: July 2, 2003Date of Patent: April 15, 2008Assignees: Duke University, E.I. du Pont de Nemours & CompanyInventors: Stephen A. Johnston, John C. Sanford
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Publication number: 20040170616Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5′ to 3′ direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: ApplicationFiled: March 5, 2004Publication date: September 2, 2004Applicant: Duke University and E.I. du Pont de Nemours & Company (Inc.)Inventors: Stephen A. Johnston, John C. Sanford
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Publication number: 20040097458Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5′ to 3′ direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: ApplicationFiled: July 2, 2003Publication date: May 20, 2004Applicant: DUKE UNIVERSITY AND E.I. DU PONT DE NEMOURS & COMPANY (INC.)Inventors: Stephen A. Johnston, John C. Sanford
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Publication number: 20040092019Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5′ to 3′ direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: ApplicationFiled: July 2, 2003Publication date: May 13, 2004Applicants: DUKE UNIVERSITY, E.I. DU PONT DE NEMOURS & COMPANY (INC.)Inventors: Stephen A. Johnston, John C. Sanford
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Patent number: 6570067Abstract: The invention provides a method for producing stably transformed dicotyledonous plants, via the introduction of a foreign DNA, containing an expression vector carrying a gene of interest, into dicotyledonous cells via one or more microparticle bombardments to produce transformed dicotyledonous cells; followed by the regeneration of a stably transformed fertile dicotyledonous plant from the transformed dicotyledonous cells.Type: GrantFiled: July 30, 1999Date of Patent: May 27, 2003Assignee: Pioneer Hi-Bred International, Inc.Inventors: Dwight T. Tomes, Arthur Weissinger, John C. Sanford, Theodore M. Klein
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Patent number: 6365396Abstract: A method for conferring resistance to a parasite to a host of the parasite, which comprises isolating a gene fragment from the parasite and inserting the gene fragment or a DNA or RNA segment substantially homologous to the gene fragment or to a DNA or RNA sequence functionally equivalent to the gene fragment into the host, wherein (1) transcription of the gene fragment or the DNA or RNA segment in the host occurs in an anti-sense direction, (2) the gene fragment or the DNA or RNA segment is expressed as a gene product in the host, wherein the gene product is capable of disrupting an essential activity of the parasite, or (3) the gene fragment or the DNA or RNA segment is a binding site capable of competing with a native binding site in the parasite, is disclosed along with hosts produced by this process. Particularly preferred is conferring resistance using a gene fragment from a replicase gene of an RNA virus.Type: GrantFiled: May 20, 1999Date of Patent: April 2, 2002Assignee: Cornell Research Foundation, Inc.Inventors: Stephen A. Johnston, John C. Sanford
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Publication number: 20020006637Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5′ to 3′ direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: ApplicationFiled: January 8, 2001Publication date: January 17, 2002Inventors: Stephen A. Johnston, John C. Sanford
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Patent number: 6258999Abstract: Fertile, stably transformed plants regenerated from culture, produced by one or more bombardments of target cells or tissues with microprojectiles coated with foreign DNA comprising one or more genes coding for a desired trait, and selection of transformed cells in culture, are provided.Type: GrantFiled: May 16, 1995Date of Patent: July 10, 2001Assignee: Pioneer Hi-Bred International, Inc.Inventors: Dwight T. Tomes, Arthur Weissinger, John C. Sanford, Theodore M. Klein
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Patent number: 6194389Abstract: A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5′ to 3′ direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.Type: GrantFiled: April 11, 1997Date of Patent: February 27, 2001Assignees: Duke University, E.I. du Pont de Nemours & Company, Inc, Cornell Research Foundation, Inc.Inventors: Stephen A. Johnston, R. Sanders Williams, John C. Sanford, Sandra G. McElligott
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Patent number: 5990387Abstract: The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.Type: GrantFiled: October 6, 1994Date of Patent: November 23, 1999Assignee: Pioneer Hi-Bred International, Inc.Inventors: Dwight T. Tomes, Arthur Weissinger, John C. Sanford, Theodore M. Klein
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Patent number: 5886244Abstract: Fertile, stably transformed Zea mays plants can be produced by introducing agronomic trait-encoding DNA, via microprojectile bombardment, into maize embryonic callus or suspension culture that has been broken into clumps having a maximum diameter of about 650 microns, where the bombardment is effected within six days of the formation of the clumps.Type: GrantFiled: May 15, 1998Date of Patent: March 23, 1999Assignee: Pioneer Hi-Bred International, Inc.Inventors: Dwight T. Tomes, Arthur Weissinger, John C. Sanford, Theodore M. Klein
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Patent number: 5840481Abstract: A method for conferring resistance to a parasite to a host of the parasite, which comprises isolating a gene fragment from the parasite and inserting the gene fragment or a DNA or RNA segment substantially homologous to the gene fragment or to a DNA or RNA sequence functionally equivalent to the gene fragment into the host, wherein (1) transcription of the gene fragment or the DNA or RNA segment in the host occurs in an anti-sense direction, (2) the gene fragment or the DNA or RNA segment is expressed as a gene product in the host, wherein the gene product is capable of disrupting an essential activity of the parasite, or (3) the gene fragment or the DNA or RNA segment is a binding site capable of competing with a native binding site in the parasite, is disclosed along with hosts produced by this process. Particularly preferred is conferring resistance using a gene fragment from a replicase gene of an RNA virus.Type: GrantFiled: November 29, 1996Date of Patent: November 24, 1998Assignees: Cornell Research Foundation, Inc., Stephen A. JohnstonInventors: Stephen A. Johnston, John C. Sanford
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Patent number: 5580716Abstract: A method for conferring resistance to a parasite to a host of the parasite, which comprises isolating a gene fragment from the parasite and inserting the gene fragment or a DNA or RNA segment substantially homologous to the gene fragment or to a DNA or RNA sequence functionally equivalent to the gene fragment into the host, wherein (1) transcription of the gene fragment or the DNA or RNA segment in the host occurs in an anti-sense direction, (2) the gene fragment or the DNA or RNA segment is expressed as a gene product in the host, wherein the gene product is capable of disrupting an essential activity of the parasite, or (3) the gene fragment or the DNA or RNA segment is a binding site capable of competing with a native binding site in the parasite, is disclosed along with hosts produced by this process. Particularly preferred is conferring resistance using a gene fragment from a replicase gene of an RNA virus.Type: GrantFiled: November 17, 1994Date of Patent: December 3, 1996Assignees: Stephen A. Johnston, Cornell Research Foundation, Inc.Inventors: Stephen A. Johnston, John C. Sanford
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Patent number: 5580761Abstract: In accordance with the present invention, disclosed is a method of conferring, upon a host cell, resistance to retroviral infection by interfering with one or more of the infection processes including retroviral replication and assembly into infective viral particles. The method involves introducing a vector into a host cell, wherein the vector comprises a polynucleotide which directs transcription, within the host cell, of RNA which is a) complementary or corresponding, depending on the target region, to a nucleic acid sequence within one or more regions of the genome of the retrovirus; and b) is effective in inhibiting one or more steps in the retroviral infection process by interfering with retroviral replication, reverse transcription, translation, or assembly into viral particles when the host cell is infected. Also disclosed is a method of treatment using the nucleic acid constructs, or cells upon which resistance to infection has been conferred.Type: GrantFiled: March 23, 1994Date of Patent: December 3, 1996Assignee: Greatbatch Gen-Aid Ltd.Inventors: Wilson Greatbatch, John C. Sanford
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Patent number: PP11746Abstract: A new and distinct cultivar of red raspberry plant named ‘Encore’ matures a high percentage of fruit from late July to early August in the East Coast and Great Lakes regions, thereby extending the summer harvest season and decreasing the gap between the summer and fall harvest seasons.Type: GrantFiled: October 6, 1998Date of Patent: January 23, 2001Assignee: Cornell Research Foundation, Inc.Inventors: John C. Sanford, Kevin E. Maloney, Jack E. Reich, Donald K. Ourecky
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Patent number: PP11747Abstract: A new and distinct cultivar of red raspberry plant named ‘Prelude’ is a very early maturing summer red raspberry cultivar available for production in the East Coast and Great Lakes regions, peaking in production in late June and very early July.Type: GrantFiled: October 6, 1998Date of Patent: January 23, 2001Assignee: Cornell Research Foundation, Inc.Inventors: John C. Sanford, Kevin E. Maloney, Jack E. Reich, Donald K. Ourecky
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Patent number: PP16480Abstract: This invention relates to a new and distinct June-bearing cultivar of strawberry plant named ‘L'Amour’ primarily adapted to the growing conditions of west central New York and other regions of similar climate. The new cultivar is primarily characterized by strong vigor, large flowers, longer than broad fruit, conical fruit shape, uniformity in shape between primary and secondary fruit, bright red fruit color, glossy skin, very firm fruit flesh, large reflexed calyx, and long pedicels and fruiting trusses.Type: GrantFiled: June 21, 2004Date of Patent: April 25, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Courtney A. Weber, John C. Sanford, Kevin E. Maloney
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Patent number: PP16571Abstract: This invention relates to a new and distinct June-bearing cultivar of strawberry plant named ‘Clancy’ primarily adapted to the growing conditions of west central New York and other regions of similar climate. The new cultivar is primarily characterized by strong vigor, large flowers, broader than long fruit, globose fruit shape, very large primary fruit, dark red fruit color, very firm fruit flesh, the upright attitude of pedicels and fruiting trusses, and late season fruit maturation.Type: GrantFiled: June 21, 2004Date of Patent: May 23, 2006Assignee: Cornell Research Foundation Inc.Inventors: Courtney A. Weber, John C. Sanford, Kevin E. Maloney